VIM, NDM, IMP, GES, SPM, GIM, SIM Metallobetalactamases in Carbapenem-Resistant Pseudomonas Aeruginosa Isolates from a Turkish University Hospital

Abstract

Background: Pseudomonas aeruginosa is an important opportunistic pathogen, and carbapenem resistance is an emerging problem. The determination of resistance genes is vital for epidemiological purposes, and the early determination of carbapenemase production methods is also recommended. Objectives: The present study aimed to investigate the presence of VIM, NDM, IMP, GES, SPM, GIM, and SIM genes and study the results of the blue carba test as a phenotypic carbapenamase detection method in P. aeruginosa. Methods: In this study, 200 carbapenem-resistant P. aeruginosa isolates were included. The DNA extraction of the carbapenem-resistant isolates was performed using the boiling method. Following the DNA extraction, optimization was conducted using the original primers. After optimization, the VIM, NDM, IMP, GES, SPM, GIM, and SIM genes were examined using the polymerase chain reaction (PCR) method. Results: The isolates were mainly identified from the tracheal aspirate cultures (34.5%). The PCR method revealed the presence of VIM in one of the P. aeruginosa isolates, and the NDM gene in one isolate using. None of the isolates was positive in terms of the IMP, GES, SPM, GIM, and SIM genes. Conclusions: In our study, two carbapenemase genes (VIM and NDM) were detected in the P. aeruginosa isolates.