A Biochemical Study on the Effects of Fullerene C60 and Fruit Extract of Balanites aegyptica Plant in Ameliorating the Toxicity Induced by Doxorubicin in Streptozotocin Model of Diabetic Male Albino Rats

Abstract

There were relationship between hyperglycaemic and pharmakinetic of toxicity of doxorubicin that lead to increase oxidative stress on cells. This study was aimed to detect the curative effect of some antioxidants drugs (fullerene C60 and mesocarp fruit extract of Balanites aegyptaica) on the haematological and biochemical of kidney function parameters after induced by diabetes and toxicity with doxorubicin on the rats. Nine groups of adult male albino rats were established (n=8). The first group (Group 1) was served as normal group. Group 2 was injected intraperitoneal (i.p) with by streptozotocin at single dose (45 mg/kg body weight). Diabetic rats (Group 3) were injected intraperitoneal (i.p) with doxorubicin at dose (5 mg/kg body weight) for three days. Diabetic rats (Group 4) were given orally fullerene C60 daily at dose (4 mg/kg body weight). Diabetic rats (Group 5) treated orally by mesocarp fruit extract of Balanites aegyptaica at dose (1.5 ml/kg body weight). (Group 6) treated orally with fullerene C60 plus mesocarp fruit extract of Balanites aegyptaica. Diabetic rats (Group 7) intoxicated DOX treated orally with fullerene C60. Diabetic rats (Group 8) intoxicated DOX treated orally with mesocarp fruit extract of Balanites aegyptaica. Diabetic rats (Group 9) diabetic rats treated orally with fullerene C60 plus mesocarp fruit extract of Balanites aegyptaica. Whole blood and serum were collected for haematological and biochemical of kidney functions examinations, respectively. Diabetes induce, hematotoxicity was determined by a highly significant decrease in Red Blood Cells (RBCs), White Blood Cells (WBCs), Blood Platelets (PLTs), Haemoglobin (Hb) and PCV value, as well as nephropathy was determined by a highly significant increase in creatinne, urea and uric acid. Diabetic rats intoxicated DOX showed reduction in haematological parameters, also, there were highly significant increases in kidney function parameters. Fullerene C60 and mesocarp fruit extract of Balanites aegyptaica ameliorated haematological and kidney functions indices. It could be concluded that Fullerene C60 and Balanites aegyptiaca, clarified a modulatory role against the cellular damage produced by oxidative stress.