Phylogenetic Analysis of Streptomyces sp. H2AK isolated from soil in Kuşadası

Abstract

Streptomyces is a genus of Gram-positive bacteria that has a filamentous form similar to fungi. Streptomyces produces bioactive secondary metabolites, such as antifungals, antivirals, antitumorals and various antibiotics. The aim of this study is to carry out phylogenetic analysis of Streptomyces sp. H2AK isolated from soil sample taken from the Kuşadası located near Aydın province. Streptomyces sp. H2AK, was picked after 14 days of incubation at 28 ̊C on Humic acid-vitamin agar containing Cycloheximide (50 μg/ml) ve Nalidixic acid (10 μg/ml). Genomic DNA isolation was performed according to DNA isolation method [1]. The 16S rRNA gene was amplified by PCR using universal primers 27f and 1525r. Phylogenetic analyses were performed by using three different algorithms with MEGA 7 software. H2AK showed the highest 16S rRNA gene sequence similarity with Streptomyces marokkonensis Ap1T (98.90 %). When the polyphasic taxonomic analyses were completed, Streptomyces sp. H2AK isolate may be introduced into the literature as a new species of the genus Streptomyces. This study was supported by Hitit University (ODMYO19003.18.001).