What is the best laboratory method for diagnosis of Herpes Simplex Virus in genital infections

Hamed Sadegh Mazji, Z. Meshkat, M. Rezayi, S. Rezaee, E. Aryan, Hamed Gouklani, M. Fani, A. Jalili
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引用次数: 1

Abstract

Genital infection caused by Herpes simplex virus (HSV) is one of the most common health problems, worldwide. Several methods such as cell culture, serological and molecular methods have been used to detect this virus. Currently, Real-Time Polymerase Chain Reaction (Real time-PCR) technique is widely used due to its high sensitivity and specificity. Besides, Real time-PCR can be employed in the follow-up of therapeutic effects in HSV-infected person who is being treated with antiretroviral drugs. We conducted a review on traditional and current diagnostic methods with a focus on their limitations in the diagnosis of HSV infection.
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诊断生殖器感染中单纯疱疹病毒的最佳实验室方法是什么
由单纯疱疹病毒(HSV)引起的生殖器感染是全世界最常见的健康问题之一。细胞培养、血清学和分子学等方法已被用于检测该病毒。实时聚合酶链反应(Real - time-PCR)技术因其高灵敏度和特异性而被广泛应用。此外,Real - time-PCR可用于对正在接受抗逆转录病毒药物治疗的单纯疱疹病毒感染者的治疗效果的随访。我们对传统和当前的诊断方法进行了综述,重点介绍了它们在诊断HSV感染方面的局限性。
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