Detection of Ehrlichia spp. in ticks collected from stray dogs in Central and Southeastern Iran

Abstract

Ehrlichia is an etiologic agent of ehrlichiosis in humans and some animals. Rhipicephalus sanguineus is the main vector of the Ehrlichia canis and dogs, red foxes and yellow jackals are reservoirs of the bacterium. This tick has a worldwide distribution and is regarded as one of the commonest species of ticks in Iran. This research aimed to detect Ehrlichia spp. in R. sanguineus isolated from stray dogs in Central and Southeast Iran (Isfahan and Zabol), by using polymerase chain reaction (PCR) and to evaluate the prevalence of the microorganism in these two areas. Tick samples were collected from stray dogs in Isfahan and Zabol between April and June of 2018. The DNA extraction was performed with commercial kits. PCR was done to determine the 336 bp fragment related to Ehrlichia spp. Overall, 15.21% of pools in both areas were positive for Ehrlichia, 21.42% and 10% of pools were from Isfahan and Zabol respectively. The results confirmed the presence of Ehrlichia spp. in R. sanguineus in stray dogs revealing that dogs and their ticks may have a significant role in the epidemiology of the disease.