Gao Xinxin, Tian Yu, Guoliang Chen, Wu Jiawen, Bai Zhenqing
{"title":"Construction of Plant Regeneration System of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang","authors":"Gao Xinxin, Tian Yu, Guoliang Chen, Wu Jiawen, Bai Zhenqing","doi":"10.5376/MPB.2021.12.0003","DOIUrl":null,"url":null,"abstract":"In order to establish the plant regeneration system of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium for plant regeneration. The experimental results showed that MS+2.0 mg/L NAA+2.0 mg/L 6-BA was the best medium for callus induction and plant regeneration. Among these, hypocotyls and cotyledons are the best explants for callus induction. The tissue induction rate is higher than 90%. The rapid propagation system established in this study can provide the theoretical reference and practical guidance for the breeding and production of fine Glycyrrhiza uralensis Fisch. varieties.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"6 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Molecular Breeding","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5376/MPB.2021.12.0003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In order to establish the plant regeneration system of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium for plant regeneration. The experimental results showed that MS+2.0 mg/L NAA+2.0 mg/L 6-BA was the best medium for callus induction and plant regeneration. Among these, hypocotyls and cotyledons are the best explants for callus induction. The tissue induction rate is higher than 90%. The rapid propagation system established in this study can provide the theoretical reference and practical guidance for the breeding and production of fine Glycyrrhiza uralensis Fisch. varieties.