ЦИТОКИНОВЫЙ ПРОФИЛЬ ДЕНДРИТНЫХ КЛЕТОК МЫШЕЙ ПОД ВОЗДЕЙСТВИЕМ БЕЛКОВ PSEUDOMONAS AERUGINOSA OPRF И ATOX

Abstract

Aim. To study the effect of OprF and aTox proteins of Pseudomonas aeruginosa on the cytokine profile of mice dendritic cells. Materials and methods. Dendritic cells (DC) were obtained from bone marrow cells of BALB/c mice when cultured with 20 ng/ml of recombinant GM-CSF and IL-4 (Biosource, USA). OprF and aTox of P. aeruginosa were used as the inducer of maturation of DC. The level of cytokines was determined in supernatants of DC using the Bio-Plex Pro™ Mouse Cytokine 23-plex Assay (BioRad, USA). Results. Evaluation of the profile and level of cytokines produced by dendritic cells of mice demonstrates the high activity of mature DC. Under the influence of recombinant proteins OprF+aTox, both large amounts of Th-1 cytokines were synthesized: IL-1a, IL-1P, IL-6, TNF-a, Th-2 cytokines: IL- 4, IL-10, IL-13, regulatory cytokines: IL-12, IFN-y, IL-17A and chemokines: KC (CXCL1), MIP-1a (CCL3), MIP-1e (CCL4), RANTES (CCL5). In our studies, we demonstrated the possibility of obtaining mature dendritic cells from the bone marrow of mice under the influence of a complex of P. aeruginosa antigens. Conclusion. The candidate Pseudomonas aeruginosa vaccine based on its recombinant proteins OprF and aTox induces the production of chemokines and Th-1, Th-2, Th-17 cytokines by mice dendritic cells.