Evaluation of bioactive constituents in hydroalcoholic extract of Nlumbo nucifera by FTIR, HPTLC and GC-MS analysis

P. Manimekalai, C. Raj, R. Dhanalakshmi
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Abstract

The present study was aimed to determine the secondary metabolites which is present in methanolic fraction of hydro alcoholic extract of Nelumbo nucifera(G) flowers Gas Chromatography Massspectroscopy (GC-MS) method of analysis. Preliminary phytochemical screening of N. nucifera was performed for the identification of the bioactive constituents. Methanolic fraction of HPTLC finger print shows three numbers of peaks. Among the observed peaks, Peak No. 2 is having Rf value 0.88 with area 3542.4.Active constituents of Methanolic fraction of hydro alcoholic extract of Nelumbo nucifera analysed by GC-MS. Chromatogram-2 shows 19 numbers of peaks among the observed peaks, 5 peaks have greater area with quinoline structure. P.Manimekalai et al /International Journal of ChemTech Research, 2019,12(3): 103-108 DOI= http://dx.doi.org/10.20902/IJCTR.2019.120316 ***** International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.12 No.03, pp 103-108, 2019
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用FTIR、HPTLC和GC-MS分析评价牛蒡水醇提取物的活性成分
采用气相色谱-质谱(GC-MS)分析方法,对莲花醇提物甲醇组分中的次生代谢产物进行了测定。通过初步的植物化学筛选,鉴定了其生物活性成分。甲醇组分HPTLC指纹图谱有3个峰。观测峰中2号峰的Rf值为0.88,面积为3542.4。气相色谱-质谱联用分析荷叶莲醇提物甲醇组分的有效成分。在色谱图2中,有19个峰,其中有5个峰与喹啉结构的面积较大。P.Manimekalai等/International Journal of ChemTech Research, 2019,12(3): 103-108 DOI= http://dx.doi.org/10.20902/IJCTR.2019.120316 ***** International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.12 No.03, pp 103-108, 2019
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