Do Culinary Mushrooms Have Fibrinolytic Activities

S. M. Ali, Y. Tan, Jegadeesh Raman, Hariprasath Lakshmanan, T. Ling, C. Phan, V. Sabaratnam
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引用次数: 4

Abstract

Recently, many culinary mushrooms have become attractive sources of biologically but non-pharmacological activities including immune modulating activities. In this study, the fibrinolytic activities of freeze-dried basidiocarps of ten edible mushrooms were determined using a qualitative fibrin plate assay and a quantitative enzyme assay using spectrophotometric method. Further, the crude extracts of all the ten mushrooms were subjected to an aqueous two-phase system (ATPS) to recover the fibrinolytic enzymes. The crude extracts and ATPS extracts of Lentinula edodes showed the highest fibrinolytic activity of 48.06 U/mg and 54.28 U/mg, respectively assessed via the Folin-spectrophotometric method. The recovery of fibrinolytic enzymes from L. edodes was the highest and the fibrinolytic enzymes were further analysed through gel electrophoresis study. A 50 kDa sized fibrinolytic enzyme from L. edodes was revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biomed Rev 2017; 28:91-99. Keywords: edible mushroom, fibrinolytic enzyme, Lentinula edodes, aqueous two-phase system
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食用蘑菇有纤溶活性吗
最近,许多烹饪蘑菇已成为有吸引力的生物来源,但非药物活性,包括免疫调节活性。本研究采用定性纤维蛋白平板法和分光光度法测定10种食用菌冷冻干燥担子果的纤溶活性。进一步,采用两水相系统(ATPS)回收10种蘑菇粗提物中的纤溶酶。福林分光光度法测定香菇粗提物和ATPS提物的纤溶活性最高,分别为48.06 U/mg和54.28 U/mg。纤维蛋白溶解酶的回收率最高,并通过凝胶电泳进一步分析纤维蛋白溶解酶。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE),发现了一个50 kDa大小的纤维蛋白溶解酶。Biomed Rev 2017;28:91 - 99。关键词:食用菌;纤溶酶;香菇
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