S. M. Ali, Y. Tan, Jegadeesh Raman, Hariprasath Lakshmanan, T. Ling, C. Phan, V. Sabaratnam
{"title":"Do Culinary Mushrooms Have Fibrinolytic Activities","authors":"S. M. Ali, Y. Tan, Jegadeesh Raman, Hariprasath Lakshmanan, T. Ling, C. Phan, V. Sabaratnam","doi":"10.14748/BMR.V28.4454","DOIUrl":null,"url":null,"abstract":"Recently, many culinary mushrooms have become attractive sources of biologically but non-pharmacological activities including immune modulating activities. In this study, the fibrinolytic activities of freeze-dried basidiocarps of ten edible mushrooms were determined using a qualitative fibrin plate assay and a quantitative enzyme assay using spectrophotometric method. Further, the crude extracts of all the ten mushrooms were subjected to an aqueous two-phase system (ATPS) to recover the fibrinolytic enzymes. The crude extracts and ATPS extracts of Lentinula edodes showed the highest fibrinolytic activity of 48.06 U/mg and 54.28 U/mg, respectively assessed via the Folin-spectrophotometric method. The recovery of fibrinolytic enzymes from L. edodes was the highest and the fibrinolytic enzymes were further analysed through gel electrophoresis study. A 50 kDa sized fibrinolytic enzyme from L. edodes was revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biomed Rev 2017; 28:91-99. Keywords: edible mushroom, fibrinolytic enzyme, Lentinula edodes, aqueous two-phase system","PeriodicalId":8906,"journal":{"name":"Biomedical Reviews","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Reviews","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14748/BMR.V28.4454","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Recently, many culinary mushrooms have become attractive sources of biologically but non-pharmacological activities including immune modulating activities. In this study, the fibrinolytic activities of freeze-dried basidiocarps of ten edible mushrooms were determined using a qualitative fibrin plate assay and a quantitative enzyme assay using spectrophotometric method. Further, the crude extracts of all the ten mushrooms were subjected to an aqueous two-phase system (ATPS) to recover the fibrinolytic enzymes. The crude extracts and ATPS extracts of Lentinula edodes showed the highest fibrinolytic activity of 48.06 U/mg and 54.28 U/mg, respectively assessed via the Folin-spectrophotometric method. The recovery of fibrinolytic enzymes from L. edodes was the highest and the fibrinolytic enzymes were further analysed through gel electrophoresis study. A 50 kDa sized fibrinolytic enzyme from L. edodes was revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biomed Rev 2017; 28:91-99. Keywords: edible mushroom, fibrinolytic enzyme, Lentinula edodes, aqueous two-phase system