ASSESMENT OF PLATELET ANTIAGGREGANT ACTIVITY OF A FRACTION FROM AN ETHANOLIC EXTRACT OF THE BARK OF Nectandra amazonum Nees

Jenaro Antonio Espitia Corredor, L. E. C. Suárez, Mario Francisco Guerrero Pabón
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引用次数: 1

Abstract

This study shows the antiplatelet effect of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees. [N.V. “laurel amarillo”, Lauraceae] applying the Born turbidimetric method. The screening test compared the fraction of N. amazonum (0.1 mg/mL), acetylsalicylic acid (ASA 0.5 mM, as reference standard) and the DMSO (0.1%, as control) in human platelets stimulated with adenosine diphosphate (ADP 2 µM), epinephrine (EPI 2 µM), collagen (COL 1 µg/mL) and arachidonic acid (AA 0.2 mg/mL). Subsequently, the study focused to determine the antiaggregant potency of the fraction throw concentration - response curves (from 1 µg/mL to 0.4 mg/mL), obtaining the pIC50 (-log IC50) values against these platelet agonists. The results showed that whereas the control platelets attained higher percentage values of aggregation (96% AA, 89% EPI, 85% COLL, 77% ADP). N. amazonum fraction significantly reduced these effects (6% AA, 45% EPI, 10% COLL, 21% ADP), near to that obtained with ASA (17% AA, 21% EPI, 10% COLL, 20% ADP). According to concentration - response curves, the pIC50 values of the fraction gave the following order of potency: AA, 4.90 > ADP, 4.51 > COLL, 4.33 > EPI, 3.85. These results suggest that N. amazonum Nees. ethanolic fraction elicit antiplatelet effects specially related to the inhibition of arachidonic acid pathway.
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猕猴桃树皮乙醇提取物部分抗血小板聚集活性的研究
本研究显示了木荆树皮乙醇组分的抗血小板作用。(喷嘴速度“月桂”,月桂科]应用玻恩浊度法。筛选试验比较了二磷酸腺苷(ADP 2µM)、肾上腺素(EPI 2µM)、胶原蛋白(COL 1µg/mL)和花生四烯酸(AA 0.2 mg/mL)刺激人血小板后,amazon N. um (0.1 mg/mL)、乙酰水杨酸(ASA 0.5 mM,参比标准)和DMSO(0.1%,对照)的组分。随后,研究重点确定了分数浓度-响应曲线(从1µg/mL到0.4 mg/mL)的抗聚集效力,获得了对这些血小板激动剂的pIC50 (-log IC50)值。结果表明,对照组的血小板具有较高的聚集率(96% AA, 89% EPI, 85% COLL, 77% ADP)。amazon N. amazonum馏分(6% AA, 45% EPI, 10% COLL, 21% ADP)显著降低了这些效应,接近ASA (17% AA, 21% EPI, 10% COLL, 20% ADP)的效果。根据浓度-响应曲线,各组分的pIC50值效价顺序为:AA、4.90 > ADP、4.51 > COLL、4.33 > EPI、3.85。这些结果表明,亚马孙栎属植物。乙醇部分具有抗血小板作用,特别是与抑制花生四烯酸途径有关。
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