A. Atikana, K. Fujita, Alex Prima, Yopi Yopi, H. Kawasaki, Ken-ichiro Suzuki, P. Lisdiyanti
{"title":"Characterization of EPS7-like Enterobacteria phage Isolated from Indonesia","authors":"A. Atikana, K. Fujita, Alex Prima, Yopi Yopi, H. Kawasaki, Ken-ichiro Suzuki, P. Lisdiyanti","doi":"10.5454/mi.15.1.2","DOIUrl":null,"url":null,"abstract":"Bacteriophages are the most abundant entities in earth. The order is the largest and most Caudovirales widespread group among bacterial viruses. The purpose of this study was to characterize bacteriophages from Indonesian waters. During this experiment, we collected sample from Kuningan (West Java) and Buleleng (Bali), Indonesia. We used an overlay agar method with three strains of as a host (NBRC 13965, NBRC 12713 and E. coli NBRC 13168) combined with digestion profiling using three restriction enzymes (PvuII, EcoRV and HincII) and transmission electron microscope (TEM) to characterize the morphology of the phage from Indonesia. Our results showed that phage LIPI13-Bp006 is in a group of and highly similar to Enterobacteria phage EPS7.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"125 22","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Indonesia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5454/mi.15.1.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Bacteriophages are the most abundant entities in earth. The order is the largest and most Caudovirales widespread group among bacterial viruses. The purpose of this study was to characterize bacteriophages from Indonesian waters. During this experiment, we collected sample from Kuningan (West Java) and Buleleng (Bali), Indonesia. We used an overlay agar method with three strains of as a host (NBRC 13965, NBRC 12713 and E. coli NBRC 13168) combined with digestion profiling using three restriction enzymes (PvuII, EcoRV and HincII) and transmission electron microscope (TEM) to characterize the morphology of the phage from Indonesia. Our results showed that phage LIPI13-Bp006 is in a group of and highly similar to Enterobacteria phage EPS7.
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