Effect of methanolic extract of Corchorus trilocularis Linn. (Tiliaceae) in diabetic nephropathy with special reference to antioxidant activity

Abstract

Aim: Effect of methanolic extract of Corchorus trilocularis Linn. in diabetic nephropathy (DN) with special reference to anti-oxidant activity. Materials and Methods: C. trilocularis Linn. were collected from local medicinal garden of college campus and authentified by botanist. Dried leaves of selected plant were successively extracted using petroleum ether, dichloromethane, ethyl acetate, methanol, and finally water. In the previous study, methanolic extract showed potent activity so we have further selected methanolic extract for the other model. Animals were fasted for overnight then a single intra peritoneal injection of freshly prepared streptozotocin (50 mg/kg dissolved in citrate buffer pH 4.5) was injected and Nephropathy was induced by Ligation of the left renal artery. Blood was collected on 0, 14, and 28 day by retro-orbital on 28 days from eye for the estimation of biochemical parameters. The withdrawn blood was centrifuged at 5000 rpm for 10 min by using centrifuge and plasma was collected and different biochemical parameters were determined by auto analyzer. Some antioxidant parameters, that is, Malondialdehyde, glutathione, Catalase, and superoxide dismutase were also determined. Results: Here body weight of rat is decreased in DN group in comparison to normal control group while increased significantly in extract treated group at 28th day in comparison to DN group. Here blood glucose level of DN group was increased in comparison to normal control group while decreased significantly in extract treated group at 14th, 21st, and 28th days in comparison to DN group. Conclusion: The overall results of this study have clearly indicated that extract treated in a dose of 200 and 400 mg/kg ameliorate the renal ischemia reperfusion induced nephropathy in diabetic rats. The action of methanolic extract contributed by renoprotective, anti-diabetic, and antioxidant properties of C. trilocularis Linn.