Papagudi Sriram, Nicolas Kalogerakis , Leo A. Behie
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引用次数: 1
Abstract
On the basis of the theory of affinity it is shown that enzyme-linked immunosorbent assay titres cannot increase as a result of proteolysis when all the antibody proteolytic fragments have approximately the same association constants as the intact antibody for the reaction with the antigen and with the enzyme-conjugated antibody. This finding was experimentally confirmed by proteolysis experiments carried out with a monoclonal IgM antibody against Lewisb antigen using trypsin. The experimental results with this particular antibody confirmed that proteolysis cannot enhance ELISA titres in hybridoma cultures with serum-free medium or with serum-supplemented medium.
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