Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-05-24 DOI:10.1186/s43141-023-00514-9
Moh Egy Rahman Firdaus, Apon Zaenal Mustopa, Nurlaili Ekawati, Sheila Chairunnisa, Rosyida Khusniatul Arifah, Ai Hertati, Shasmita Irawan, Anika Prastyowati, Arizah Kusumawati, Maritsa Nurfatwa
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引用次数: 2

Abstract

Background: Vaccination is the one of the agendas of many countries to reduce cervical cancer caused by the Human papillomavirus. Currently, VLP-based vaccine is the most potent vaccine against HPV, which could be produced by a variety of expression systems. Our study focuses on a comparison of recombinant protein expression L1 HPV52 using two common yeasts, Pichia pastoris and Hansenula polymorpha that have been used for vaccine production on an industrial scale. We also applied bioinformatics approach using reverse vaccinology to design alternative multi-epitope vaccines in recombinant protein and mRNA types.

Results: Our study found that P. pastoris relatively provided higher level of L1 protein expression and production efficiency compared to H. polymorpha in a batch system. However, both hosts showed self-assembly VLP formation and stable integration during protein induction. The vaccine we have designed exhibited high immune activation and safe in computational prediction. It is also potentially suitable for production in a variety of expression systems.

Conclusion: By monitoring the overall optimization parameter assessment, this study can be used as the basis reference for large-scale production of the HPV52 vaccine.

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酵母衣壳蛋白L1自组装VLP的优化、表征、比较及抗人乳头瘤病毒52型的反向疫苗学设计
背景:疫苗接种是许多国家减少人乳头瘤病毒引起的宫颈癌的议程之一。目前,基于vlp的疫苗是最有效的HPV疫苗,可以通过多种表达系统生产。我们的研究重点是比较重组蛋白L1 HPV52在两种常见酵母(毕氏酵母和多态汉氏酵母)中的表达,这两种酵母已用于工业规模的疫苗生产。我们还应用生物信息学方法,利用反向疫苗学设计了重组蛋白和mRNA类型的替代多表位疫苗。结果:我们的研究发现,在批处理系统中,与多形镰刀菌相比,pastoris提供了更高的L1蛋白表达水平和生产效率。但在诱导过程中,两种宿主均表现出自组装VLP的形成和稳定的整合。我们所设计的疫苗在计算预测中表现出高的免疫激活性和安全性。它也可能适用于各种表达系统的生产。结论:通过监测整体优化参数评价,本研究可为HPV52疫苗的规模化生产提供依据参考。
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