Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie最新文献

In vitro measurements of spermatozoa migration in different media showed a 75% increase in the number of spermatozoa migrating into preovulatory human follicular fluid versus Menezo's fluid. However after 4 h storage in either medium, total and progressive motility of the spermatozoa was lower in follicular fluid than in Menezo's fluid. A positive correlation was evident between the spermatozoa migration in follicular fluid and the concentration or motility of spermatozoa in fresh sperm.

Heating of Guinea-Pig nasal mucosa fragments during 30 min. at 43 degrees inhibits the degranulation of mast cells and the liberation of histamine. This accounts for the success of the thermotherapy of persistent allergic rhinitis.

We have compared the effects of a single intrathecal injection of capsaicin or substance P (SP) on the motor responses to radiant heat and electrical nociceptive stimulations in the Rat. With nociceptive electrical stimulation, an immediate hyperalgesia followed capsaicin injection in all Rats. On the other hand, some Rats displayed hyperalgesia after SP while others showed hypoalgesia. Both capsaicin and SP, induced hypoalgesia to radiant heat in 17 rats. In 5 rats no response was obtained.

Four different continuous cell lines of T lymphocytes are described, three of them were obtained by cloning. Their activity is restricted in three cases by a histocompatibility antigen undistinguishable from HLA-DR. The last one is restricted by an autologous unidentified structure. The first three lines are specific for a viral antigen corresponding to a subtype of the H3 hemagglutinin. The fourth line seems to react with an hitherto undescribed antigen shared by type A and type B influenza viruses.

In order to study the antitumour activity of taxol (a diterpene of the taxane type) isolated from Yew: Taxus baccata L. (Taxacae), human tumours implanted as xenografts in athymic Mice were used. Swiss nude mice were treated subcutaneously (12.5 mg/kg/injection/day for 5 consecutive days out of 7, over a period of 3 weeks). Treatment by taxol of a liver metastasis of a breast tumour, a tongue primary tumour and a skin metastasis of bronchial carcinoma gave statistically significant results (0,01 greater than P greater than 0,001 and P less than 0,001). However, taxol showed a very moderate antitumour activity against a transplanted primary tumour of the colon.

In vivo binding of a benzodiazepine (flunitrazepam-C11) and a benzodiazepine antagonist (Ro 15-1788-C11) were studied with positron emission tomography. Advantages and disadvantages of each drug for studying specific in vivo binding sites are presented. The results obtained indicate that Ro 15-1788-C11 is a better in vivo radiocoordinat than flunitrazepam-C11.

We have detected a 1.6 kb RNA species in the polysomes of cells infected with the m1 strain of Moloney Murine Sarcoma Virus. This RNA contains the cellular transforming sequences (mos) as well as sequences located at both ends of the viral genome and is devoid of other internal viral sequences. This RNA appears to be spliced and might function as the messenger for the transforming protein of the Moloney Murine Sarcoma Virus.

Oxolinic acid inhibits the appearance of transconjugants between Escherichia coli harbouring RP4/R6K plasmids and recipient strains. This effect is detected from 1 microgram/ml, (3, 8 microM) without loss of viability, and is not due to a curing process.

A system exchanging extracellular Na+ against intracellular Na+ in a one to one ratio has been characterized in human erythrocyte. The function of this system is not yet shown, however its activity is altered in some hypertensive or manic depressives. Its activity can be determined by measuring the rate of Na+ flux in the presence in the extracellular medium, of lithium, ouabain and bumetanide. It is inhibited by phloretin. In this work, a similar flux has been demonstrated in Rat erythrocyte.

Two fractions, characterized by biochemical criteria [quantity and density of particulate material, localization of myelin specific protein--Po, P1 and Pr--and of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) and of 5'-nucleotidase], were isolated from sciatic nerve homogenate of normal, Trembler and quaking, young and adult Mice, on a continuous sucrose gradient. The fraction, at 0.58-0.6 M, the richest in membrane particles contains the myelin. The fraction at 0.7-0.75 M should contain the plasma membrane of the Schwann cell which synthesizes myelin.