Soudabe Behrooj, Moazameh Mohammadi Soleimani, Hossein Farshidi, Abdullah Gharibzadeh, D. Hooshyar
Background: The COVID-19 pandemic has raised a serious challenge for health care systems, a challenge which requires taking effective and intensive measures to provide patient care. COVID-19 can cause damages to various organs, including heart, through causing various changes in the inflammatory and coagulation systems. Some cases of cardiac injury can display mistakable signs of myocardial infarction (MI). Cardiac injury can mimic acute conditions such as MI. Case Presentation: In this study, a case of a 33-year-old man with an initial diagnosis of MI by ST-elevation was investigated. He later developed pulmonary thromboembolism (PTE) after being treated with fibrinolytic and anticoagulants; after further investigations, however, he was found not afflicted with primary MI. Our findings may have proven useful in demonstrating the unexpected effects of anticoagulants on COVID-19. Conclusion: Miss-diagnosing these cases as well as administrating effective treatment for COVID-19 patients may have posed real risks to the patients and made it difficult to manage them due to the high risk of death and the lack of differential diagnosis of the given patients.
{"title":"Fountain of Youth or the Trojan Horse: A Case Report of Pulmonary Thromboembolism After Anticoagulant Administration in a COVID-19 Patient","authors":"Soudabe Behrooj, Moazameh Mohammadi Soleimani, Hossein Farshidi, Abdullah Gharibzadeh, D. Hooshyar","doi":"10.34172/ddj.2023.378","DOIUrl":"https://doi.org/10.34172/ddj.2023.378","url":null,"abstract":"Background: The COVID-19 pandemic has raised a serious challenge for health care systems, a challenge which requires taking effective and intensive measures to provide patient care. COVID-19 can cause damages to various organs, including heart, through causing various changes in the inflammatory and coagulation systems. Some cases of cardiac injury can display mistakable signs of myocardial infarction (MI). Cardiac injury can mimic acute conditions such as MI. Case Presentation: In this study, a case of a 33-year-old man with an initial diagnosis of MI by ST-elevation was investigated. He later developed pulmonary thromboembolism (PTE) after being treated with fibrinolytic and anticoagulants; after further investigations, however, he was found not afflicted with primary MI. Our findings may have proven useful in demonstrating the unexpected effects of anticoagulants on COVID-19. Conclusion: Miss-diagnosing these cases as well as administrating effective treatment for COVID-19 patients may have posed real risks to the patients and made it difficult to manage them due to the high risk of death and the lack of differential diagnosis of the given patients.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86324962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Although today, transfusion therapy has turned from a hazardous condition to a relatively safe method with the improvement of technology, the highest risk for the patient is a complication of blood transfusions. This study aimed to evaluate the prevalence and pattern of transfusion-related adverse events. Materials and Methods: This study is a retrospective review which examined data of all those who received blood transfusions at Kashani and Imam Khomeini hospitals of the south of Kerman province from 2014 to 2018 years. Results: Of the total 16081 blood transfusions, 148 (0.92%) cases of blood complications were observed. Most cases occurred in males aged 31-40 and over 50 years, O blood group, injection packed red blood cell (PRBC) product, and within 20-60 minutes. Most of the transfusion-related complications were related to the surgery ward and in patients without underlying disease, and most complications included fever and chills. Conclusion: The results of our study showed that the highest transfusion-related complications were fever and chills. Therefore, fever and chills in the early hours of transfusion should be considered as the most common transfusion-related complications.
{"title":"Noninfectious Complications of Blood Transfusion in the South of Kerman Province: A 4-Year Retrospective Study","authors":"Nosaibe Seirfar, Javad Afsharmanesh, Amin Sadeghi dousari, Ali Behzadi, Roohollah Mirzaee Khalilabad","doi":"10.34172/ddj.2022.21","DOIUrl":"https://doi.org/10.34172/ddj.2022.21","url":null,"abstract":"Background: Although today, transfusion therapy has turned from a hazardous condition to a relatively safe method with the improvement of technology, the highest risk for the patient is a complication of blood transfusions. This study aimed to evaluate the prevalence and pattern of transfusion-related adverse events. Materials and Methods: This study is a retrospective review which examined data of all those who received blood transfusions at Kashani and Imam Khomeini hospitals of the south of Kerman province from 2014 to 2018 years. Results: Of the total 16081 blood transfusions, 148 (0.92%) cases of blood complications were observed. Most cases occurred in males aged 31-40 and over 50 years, O blood group, injection packed red blood cell (PRBC) product, and within 20-60 minutes. Most of the transfusion-related complications were related to the surgery ward and in patients without underlying disease, and most complications included fever and chills. Conclusion: The results of our study showed that the highest transfusion-related complications were fever and chills. Therefore, fever and chills in the early hours of transfusion should be considered as the most common transfusion-related complications.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"441 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78172289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Pneumonia is one of the leading causes of diseases in children that remains a serious threat. This study aimed to investigate the laboratory and inflammatory markers in children with pneumonia and their relationship with the severity of the disease. Materials and Methods: This retrospective descriptive-cross-sectional study included 400 children with pneumonia patients, aged 1 month to 12 years, who were referred to Bandar Abbass Children’s Hospital. In this study, the severity of the disease was compared with variables such as age, gender, white blood cell (WBC) count, neutrophil percentage, erythrocyte sedimentation rate (ESR), and community-acquired pneumonia (CAP). Results: Of the 400 people who participated in the survey, 57.5% and 42.5% of cases were males and females, respectively. The mean age of the studied patients was 16.53±21.57 months. There was no significant relationship between the severity of pneumonia and leukocyte count, neutrophil count, and inflammatory markers (P>0.05). Conclusion: Based on the findings, the severity of the disease was mild to moderate in 90% of patients. ESR, C-reactive protein (CRP), leukocyte count, and neutrophil count were not significantly associated with the severity of pneumonia in children (P>0.05).
{"title":"The Relationship of Leukocyte and Neutrophil Count, C-Reactive Protein, and Erythrocyte Sedimentation Rate With the Severity of Childhood Pneumonia Without Conjugated Pneumococcal Vaccine Use","authors":"M. Rahmati","doi":"10.34172/ddj.2022.17","DOIUrl":"https://doi.org/10.34172/ddj.2022.17","url":null,"abstract":"Background: Pneumonia is one of the leading causes of diseases in children that remains a serious threat. This study aimed to investigate the laboratory and inflammatory markers in children with pneumonia and their relationship with the severity of the disease. Materials and Methods: This retrospective descriptive-cross-sectional study included 400 children with pneumonia patients, aged 1 month to 12 years, who were referred to Bandar Abbass Children’s Hospital. In this study, the severity of the disease was compared with variables such as age, gender, white blood cell (WBC) count, neutrophil percentage, erythrocyte sedimentation rate (ESR), and community-acquired pneumonia (CAP). Results: Of the 400 people who participated in the survey, 57.5% and 42.5% of cases were males and females, respectively. The mean age of the studied patients was 16.53±21.57 months. There was no significant relationship between the severity of pneumonia and leukocyte count, neutrophil count, and inflammatory markers (P>0.05). Conclusion: Based on the findings, the severity of the disease was mild to moderate in 90% of patients. ESR, C-reactive protein (CRP), leukocyte count, and neutrophil count were not significantly associated with the severity of pneumonia in children (P>0.05).","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84455438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Dyslipidemia is one of the main risk factors for coronary artery disease, stroke, and peripheral vascular disease. This study aimed to evaluate the effect of eight weeks of incremental pump body training on some atherogenic factors in obese women with dyslipidemia. Materials and Methods: In this quasi-experimental study, 40 obese female subjects in the age range of 30-50 years suffering from dyslipidemia were randomly divided into two groups of body pump training (n=20) and control (n=20). The body pump training program consisted of eight weeks with a frequency of 3 sessions per week for 45 minutes with overload and 48 hours of rest. Data were analyzed using independent samples t test and paired samples t test. Results: The results showed that eight weeks of body pump training significantly decreased triglyceride, cholesterol, low-density lipoprotein (LDL), and atherogenic index (AI) in obese women with dyslipidemia, while there was a significant increase in high-density lipoprotein (HDL) (P˂0.001). Conclusion: The results of this study show that body pump training can properly prevent the progression of dyslipidemia in people suffering this complication.
{"title":"The Effect of Eight Weeks of Incremental Pump Body Training on Some Atherogenic Factors in Obese Women With Dyslipidemia","authors":"Atefeh Lailaei, Morvarid Vafaei, B. Abedi","doi":"10.34172/ddj.2022.22","DOIUrl":"https://doi.org/10.34172/ddj.2022.22","url":null,"abstract":"Background: Dyslipidemia is one of the main risk factors for coronary artery disease, stroke, and peripheral vascular disease. This study aimed to evaluate the effect of eight weeks of incremental pump body training on some atherogenic factors in obese women with dyslipidemia. Materials and Methods: In this quasi-experimental study, 40 obese female subjects in the age range of 30-50 years suffering from dyslipidemia were randomly divided into two groups of body pump training (n=20) and control (n=20). The body pump training program consisted of eight weeks with a frequency of 3 sessions per week for 45 minutes with overload and 48 hours of rest. Data were analyzed using independent samples t test and paired samples t test. Results: The results showed that eight weeks of body pump training significantly decreased triglyceride, cholesterol, low-density lipoprotein (LDL), and atherogenic index (AI) in obese women with dyslipidemia, while there was a significant increase in high-density lipoprotein (HDL) (P˂0.001). Conclusion: The results of this study show that body pump training can properly prevent the progression of dyslipidemia in people suffering this complication.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79975237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maryam Esteghamati, A. Moayedi, Somayeh Jalilzadeh, Ghazal Zoghi
Background: Alterations in hematological and renal parameters have been reported with antiepileptic drugs (AEDs). This study aimed to evaluate the effects of lamotrigine (LTG) and levetiracetam (LEV) on these parameters in children with epilepsy. Materials and Methods: This randomized clinical trial included children with a first-time diagnosis of epilepsy referred to Bandar Abbas Children’s Hospital, Bandar Abbas, Iran, from 2017 to 2018. Participants’ age, gender, and family history of epilepsy were recorded at the time of admission. Patients in the LTG group received 0.6 mg/kg of oral LTG in two divided doses for two weeks which continued with 1.2 mg/kg for another two weeks and then with a maintenance dose of 5-15 mg/kg daily. Patients in the LEV group received 10 mg/kg of oral LEV twice a day. When necessary, the dosage was increased to a maximum of 30 mg/kg twice a day. Treatment continued until seizures were controlled. Hematological and renal parameters were measured at baseline and 3 months after treatment. The total duration of treatment with each drug was noted as well. Results: Of the 66 children evaluated in this study with a mean age of 8.51±2.11 years, 31 (47%) were males. Age, gender, family history of epilepsy, treatment duration, and baseline hematological and renal parameters did not differ between the LTG (n=26) and LEV (n=40) groups. Patients in both groups were comparable regarding all the parameters after treatment. Finally, no significant change was observed after treatment compared to baseline in either group. Conclusion: Overall, LTG and LEV appear to have no significant effect on the hematological and renal parameters of children with epilepsy.
{"title":"Hematological and Renal Effects of Levetiracetam Versus Lamotrigine in Children With Epilepsy: A Randomized Clinical Trial","authors":"Maryam Esteghamati, A. Moayedi, Somayeh Jalilzadeh, Ghazal Zoghi","doi":"10.34172/ddj.2022.20","DOIUrl":"https://doi.org/10.34172/ddj.2022.20","url":null,"abstract":"Background: Alterations in hematological and renal parameters have been reported with antiepileptic drugs (AEDs). This study aimed to evaluate the effects of lamotrigine (LTG) and levetiracetam (LEV) on these parameters in children with epilepsy. Materials and Methods: This randomized clinical trial included children with a first-time diagnosis of epilepsy referred to Bandar Abbas Children’s Hospital, Bandar Abbas, Iran, from 2017 to 2018. Participants’ age, gender, and family history of epilepsy were recorded at the time of admission. Patients in the LTG group received 0.6 mg/kg of oral LTG in two divided doses for two weeks which continued with 1.2 mg/kg for another two weeks and then with a maintenance dose of 5-15 mg/kg daily. Patients in the LEV group received 10 mg/kg of oral LEV twice a day. When necessary, the dosage was increased to a maximum of 30 mg/kg twice a day. Treatment continued until seizures were controlled. Hematological and renal parameters were measured at baseline and 3 months after treatment. The total duration of treatment with each drug was noted as well. Results: Of the 66 children evaluated in this study with a mean age of 8.51±2.11 years, 31 (47%) were males. Age, gender, family history of epilepsy, treatment duration, and baseline hematological and renal parameters did not differ between the LTG (n=26) and LEV (n=40) groups. Patients in both groups were comparable regarding all the parameters after treatment. Finally, no significant change was observed after treatment compared to baseline in either group. Conclusion: Overall, LTG and LEV appear to have no significant effect on the hematological and renal parameters of children with epilepsy.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"30 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82966753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Salimi Asl, F. Malekzadeh, A. Nikpoor, Shaghayegh Kermaninezhad, Sobhan Montazerghaem
The new coronavirus was first identified in China in December 2019 and then, it created a widespread pandemic around the world. There is currently no definitive cure for it and it will not be possible to make a vaccine soon. Pathogenic mechanism of the virus includes virus entry, binding to angiotensin-converting enzyme 2 (ACE2) receptors on the surface of host cells, proliferation, membrane fusion, assembly, subsequent infection, cytokine secretion, and the host immune response to the virus. By identifying at what stage the patient is, possible effective drugs can be used to control each stage. Therefore, identifying the mechanism of action of the virus in different stages of the disease makes it possible to use the appropriate drug for each stage of the disease and control the disease.
{"title":"COVID-19 Treatments Based on Pathogenicity","authors":"Ali Salimi Asl, F. Malekzadeh, A. Nikpoor, Shaghayegh Kermaninezhad, Sobhan Montazerghaem","doi":"10.34172/ddj.2022.23","DOIUrl":"https://doi.org/10.34172/ddj.2022.23","url":null,"abstract":"The new coronavirus was first identified in China in December 2019 and then, it created a widespread pandemic around the world. There is currently no definitive cure for it and it will not be possible to make a vaccine soon. Pathogenic mechanism of the virus includes virus entry, binding to angiotensin-converting enzyme 2 (ACE2) receptors on the surface of host cells, proliferation, membrane fusion, assembly, subsequent infection, cytokine secretion, and the host immune response to the virus. By identifying at what stage the patient is, possible effective drugs can be used to control each stage. Therefore, identifying the mechanism of action of the virus in different stages of the disease makes it possible to use the appropriate drug for each stage of the disease and control the disease.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77749178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Biofilms are a major challenge in treating infections. Clinically, biofilms are often associated with chronic infections, so rapid and accurate methods of investigating the antibiotic susceptibility of biofilm bacteria are very important for faster diagnosis and treatment. In this study, a new modified microplate method was proposed to evaluate the antibiotic susceptibility of alive bacteria in the biofilm. Materials and Methods: The study was conducted on clinical isolates of Pseudomonas aeruginosa and standard strain over a period of one year. The susceptibility test was conducted according to a modified version of the Calgary biofilm device method. In the last step, to study the living bacteria in the biofilm, oxidative-fermentative (OF) medium was used to measure the acid production following the use of glucose by the bacteria. The biofilm-growing bacteria was determined by observing the color changing and also measuring optical density (OD) at 427 nm of OF medium. Results: The method in this study could evaluate the effect of antibiotics on biofilm bacteria based on glucose metabolism. The results indicated that this method can quickly and easily identify alive bacteria in biofilm at a low cost and without the need for any special devices. Conclusion: Although biofilms are involved in most incurable clinical cases, there is currently no guideline to assist physicians in treating biofilm-related infections. Therefore, it is necessary to establish a specific sensitivity test for biofilm or to approve a new method for routine use in diagnostic laboratories.
{"title":"An Easy New Modified Method for Detection of Antibacterial Susceptibility in Biofilm-Growing Bacteria","authors":"Foroogh Faridi, N. Bahador, S. Shoja, S. Abbasi","doi":"10.34172/ddj.2022.18","DOIUrl":"https://doi.org/10.34172/ddj.2022.18","url":null,"abstract":"Background: Biofilms are a major challenge in treating infections. Clinically, biofilms are often associated with chronic infections, so rapid and accurate methods of investigating the antibiotic susceptibility of biofilm bacteria are very important for faster diagnosis and treatment. In this study, a new modified microplate method was proposed to evaluate the antibiotic susceptibility of alive bacteria in the biofilm. Materials and Methods: The study was conducted on clinical isolates of Pseudomonas aeruginosa and standard strain over a period of one year. The susceptibility test was conducted according to a modified version of the Calgary biofilm device method. In the last step, to study the living bacteria in the biofilm, oxidative-fermentative (OF) medium was used to measure the acid production following the use of glucose by the bacteria. The biofilm-growing bacteria was determined by observing the color changing and also measuring optical density (OD) at 427 nm of OF medium. Results: The method in this study could evaluate the effect of antibiotics on biofilm bacteria based on glucose metabolism. The results indicated that this method can quickly and easily identify alive bacteria in biofilm at a low cost and without the need for any special devices. Conclusion: Although biofilms are involved in most incurable clinical cases, there is currently no guideline to assist physicians in treating biofilm-related infections. Therefore, it is necessary to establish a specific sensitivity test for biofilm or to approve a new method for routine use in diagnostic laboratories.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89513378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proliferating trichilemmal tumor (PTT) is a scarce tumor of exterior root sheath that appears to grow within the wall of a pre-existing pilar cyst in most cases. This report presents a case of malignant PTT in the posterior sacrum of a 33-year-old man presenting with limited mobility and a history of paraplegia.
{"title":"Proliferating Trichilemmal Tumor: A Case Study","authors":"N. Masoudi, M. Pashaei, F. Hosseinzadeh","doi":"10.34172/ddj.2022.24","DOIUrl":"https://doi.org/10.34172/ddj.2022.24","url":null,"abstract":"Proliferating trichilemmal tumor (PTT) is a scarce tumor of exterior root sheath that appears to grow within the wall of a pre-existing pilar cyst in most cases. This report presents a case of malignant PTT in the posterior sacrum of a 33-year-old man presenting with limited mobility and a history of paraplegia.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86526172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Epigenetics has been playing an increasingly important role in the study of the origin and development of bladder cancer (BC). This study aimed to investigate the correlation between promoter hypomethylation of the heparanase (HPSE) gene and clinicopathologic characteristics of bladder cancer (BC). Materials and Methods: The promoter hypomethylation profile was evaluated by methylation-specific polymerase chain reaction (PCR) using 27 BC tissue specimens and 15 normal control specimens. The aim was to help decipher the underlying relationship between the clinicopathologic characteristics and the hypomethylation status. Results: Experimental results showed that 16 (59.26%) BC specimens demonstrated the promoter hypomethylation of HPSE, including 2 cases with complete demethylation. For normal control groups, only 3 specimens (20%) indicated hypomethylation (P<0.05). In addition, the occurrence of hypomethylation increased with the metastasis of positive lymph nodes (P<0.05). Importantly, no significant correlation was found between the hypomethylation of HPSE and the profile of patients including gender, age, tumor size, cancer stage, or histologic grade (P>0.05). Conclusion: The promoter hypomethylation of HPSE gene is a common epigenetic event occurring in BC and is positively correlated with a poor prognosis. This study suggested that the promoter hypomethylation could be used as a potential biological marker for the early screening of BC.
{"title":"Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer","authors":"Bai-sheng Xu, Yan-ying Jiang, Caizhi Liao, M. Yan","doi":"10.34172/ddj.2022.19","DOIUrl":"https://doi.org/10.34172/ddj.2022.19","url":null,"abstract":"Background: Epigenetics has been playing an increasingly important role in the study of the origin and development of bladder cancer (BC). This study aimed to investigate the correlation between promoter hypomethylation of the heparanase (HPSE) gene and clinicopathologic characteristics of bladder cancer (BC). Materials and Methods: The promoter hypomethylation profile was evaluated by methylation-specific polymerase chain reaction (PCR) using 27 BC tissue specimens and 15 normal control specimens. The aim was to help decipher the underlying relationship between the clinicopathologic characteristics and the hypomethylation status. Results: Experimental results showed that 16 (59.26%) BC specimens demonstrated the promoter hypomethylation of HPSE, including 2 cases with complete demethylation. For normal control groups, only 3 specimens (20%) indicated hypomethylation (P<0.05). In addition, the occurrence of hypomethylation increased with the metastasis of positive lymph nodes (P<0.05). Importantly, no significant correlation was found between the hypomethylation of HPSE and the profile of patients including gender, age, tumor size, cancer stage, or histologic grade (P>0.05). Conclusion: The promoter hypomethylation of HPSE gene is a common epigenetic event occurring in BC and is positively correlated with a poor prognosis. This study suggested that the promoter hypomethylation could be used as a potential biological marker for the early screening of BC.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"27 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83679239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ordooei, Zahra Niknafs, A. Mehrabbeik, N. Namiranian
Background: Chronic diseases such as diabetes have significant effects on the mental and social health of patients. The aim of this study was to investigate the mental and social health status of children with type 1 diabetes mellitus (T1DM). Materials and Methods: This cross-sectional study was conducted on 52 T1 diabetic patients aged 12-18 years who referred to Yazd Diabetes Center in 2020. The patients were selected by simple random sampling. The data collection tool was the Pediatric Symptom Checklist (PSC) questionnaire, which contained 35 three-choice psychological questions about mental health. Data were analyzed using SPSS 22. Results: Totally, 31 (56.9%) and 21 (40.4%) of patients were males and females, respectively. The mean age of the subjects was 15.23 (±2) and the mean duration of diabetes was 6.19 (±3.56) years. Moreover, 48.1% of patients had a positive family history of diabetes. The mean of hemoglobin A1c (HbA1c) was 8.05 (±1.62) and the mean of HbA1c in patients with psychosocial disorders was 8.74 (±0.08), which was significantly higher than that of subjects without psychosocial disorders (P=0.032). Conclusion: T1DM patients, whose blood sugar was not controlled, reported more mental health problems, thus it is essential to apply psychosocial health promotion strategies to these adolescents and their families.
{"title":"Mental and Social Health Status and its Association With Glycosylated Hemoglobin Level in Adolescents Aged 12- 18 Years With Type 1 Diabetes","authors":"M. Ordooei, Zahra Niknafs, A. Mehrabbeik, N. Namiranian","doi":"10.34172/ddj.2022.11","DOIUrl":"https://doi.org/10.34172/ddj.2022.11","url":null,"abstract":"Background: Chronic diseases such as diabetes have significant effects on the mental and social health of patients. The aim of this study was to investigate the mental and social health status of children with type 1 diabetes mellitus (T1DM). Materials and Methods: This cross-sectional study was conducted on 52 T1 diabetic patients aged 12-18 years who referred to Yazd Diabetes Center in 2020. The patients were selected by simple random sampling. The data collection tool was the Pediatric Symptom Checklist (PSC) questionnaire, which contained 35 three-choice psychological questions about mental health. Data were analyzed using SPSS 22. Results: Totally, 31 (56.9%) and 21 (40.4%) of patients were males and females, respectively. The mean age of the subjects was 15.23 (±2) and the mean duration of diabetes was 6.19 (±3.56) years. Moreover, 48.1% of patients had a positive family history of diabetes. The mean of hemoglobin A1c (HbA1c) was 8.05 (±1.62) and the mean of HbA1c in patients with psychosocial disorders was 8.74 (±0.08), which was significantly higher than that of subjects without psychosocial disorders (P=0.032). Conclusion: T1DM patients, whose blood sugar was not controlled, reported more mental health problems, thus it is essential to apply psychosocial health promotion strategies to these adolescents and their families.","PeriodicalId":11143,"journal":{"name":"Disease and Diagnosis","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78724074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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