Hadis Moradi, Ahmad Motaghi, A. Aminzadeh, Alireza Sadighi, Mohammad Hossien Shafazand
CD4 T lymphocytes play a central role in allergic reactions. Thus the present study aimed to, immunohistochemically, evaluate the presence of these lymphocytes in rabbit gingival tissues after the replacement of Cenobone. This experimental one way blinded study was performed on 20 gingival tissues gathered from disease-free rabbits with or without bone powder, respectively groups A and B. Immunohistochemical envision method was performed for mapping CD4 lymphocytes. The number and intensity of staining were compared between groups in 5 consequent HPF without overlap with the light microscope in connective tissue. Data were analyzed by Fisher exact test, Wilcoxon, and chi-square statistically in SPSS20 software. The number of CD4 T cells was higher in group A compared to group B.(P=0.02) Pattern of distribution in connective tissue did not show a difference between the two groups. (P=0.41). Results of the present study might confirm the role of CD4 T in an allergic reaction to bone powder material and suggest this cell as a useful factor for the prediction of allergic reactions in the first weeks of surgery. Further studies in this field are required.
{"title":"Immunohistochemical Evaluation of Gingival CD4+ lymphocytes in Response to Allograft Bone Powder (Ceno Bone) - Animal Study","authors":"Hadis Moradi, Ahmad Motaghi, A. Aminzadeh, Alireza Sadighi, Mohammad Hossien Shafazand","doi":"10.12944/edj.03.01.05","DOIUrl":"https://doi.org/10.12944/edj.03.01.05","url":null,"abstract":"CD4 T lymphocytes play a central role in allergic reactions. Thus the present study aimed to, immunohistochemically, evaluate the presence of these lymphocytes in rabbit gingival tissues after the replacement of Cenobone. This experimental one way blinded study was performed on 20 gingival tissues gathered from disease-free rabbits with or without bone powder, respectively groups A and B. Immunohistochemical envision method was performed for mapping CD4 lymphocytes. The number and intensity of staining were compared between groups in 5 consequent HPF without overlap with the light microscope in connective tissue. Data were analyzed by Fisher exact test, Wilcoxon, and chi-square statistically in SPSS20 software. The number of CD4 T cells was higher in group A compared to group B.(P=0.02) Pattern of distribution in connective tissue did not show a difference between the two groups. (P=0.41). Results of the present study might confirm the role of CD4 T in an allergic reaction to bone powder material and suggest this cell as a useful factor for the prediction of allergic reactions in the first weeks of surgery. Further studies in this field are required.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87743046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To estimate the effectiveness of sodium perborate with carbamide-peroxide (urea peroxide) to remove the staining of non-vital discolored teeth. Comparative cross sectional study. Dental department of operative dentistry, LUMHS, Jamshoro Sindh, Pakistan, from December 15-12-2014 to June 15-6-2015. A total of 160 single rooted teeth with intact clinical crowns have been collected. Using human blood, the teeth were discolored. With the vita shade guide, shades of discolored teeth were taken. Each tooth was handled with the root canal. 2 mm of root-canal-filling material cleaned under the junction of cement-enamel. In addition, it was then coated with glass ionomer cement 1 mm thickness. Teeth were divided randomly into two groups based on bleaching content used in the pulp chamber; group-A (C.P): 16% Carbamide-peroxide. Group-B (S.P): tetra-hydrate sodium-perborate mixed with distilled water. After 21days, the results were checked for efficacy. The analysis included a total of 160 teeth extracted (80 teeth extracted in each group). In group A, 78 (51.7%) found effectiveness compared to 73 (48.3%) in group B. The experimental groups showed statistically similar bleaching results at the end of twenty one days and p value found to be not significantly different (p=0.078). With a significant result, intracoronal bleaching of non-vital colored teeth is less invasive, comparatively safe and effective to remove the staining of non-vital discolored teeth.
{"title":"Efficacy of Carbamide Peroxide and Sodium Perborate as Bleaching Agent in Non-Vital Discolored Teeth","authors":"S. Memon, F. Kalhoro","doi":"10.12944/edj.02.01.06","DOIUrl":"https://doi.org/10.12944/edj.02.01.06","url":null,"abstract":"To estimate the effectiveness of sodium perborate with carbamide-peroxide (urea peroxide) to remove the staining of non-vital discolored teeth. Comparative cross sectional study. Dental department of operative dentistry, LUMHS, Jamshoro Sindh, Pakistan, from December 15-12-2014 to June 15-6-2015. A total of 160 single rooted teeth with intact clinical crowns have been collected. Using human blood, the teeth were discolored. With the vita shade guide, shades of discolored teeth were taken. Each tooth was handled with the root canal. 2 mm of root-canal-filling material cleaned under the junction of cement-enamel. In addition, it was then coated with glass ionomer cement 1 mm thickness. Teeth were divided randomly into two groups based on bleaching content used in the pulp chamber; group-A (C.P): 16% Carbamide-peroxide. Group-B (S.P): tetra-hydrate sodium-perborate mixed with distilled water. After 21days, the results were checked for efficacy. The analysis included a total of 160 teeth extracted (80 teeth extracted in each group). In group A, 78 (51.7%) found effectiveness compared to 73 (48.3%) in group B. The experimental groups showed statistically similar bleaching results at the end of twenty one days and p value found to be not significantly different (p=0.078). With a significant result, intracoronal bleaching of non-vital colored teeth is less invasive, comparatively safe and effective to remove the staining of non-vital discolored teeth.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85366553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As per the published literature till September 2020, the only oral manifestations of the Covid infection are loss of smell and taste sensation. These could be due to the direct effects of tropism of virus to tongue and oral mucous membrane.2, 3 Lateron there were emergence of secondary infections and various oral manifestations varying from ulcers to devastating fungal infections like mucormycosis.
{"title":"An Epidemic during Covid 19 Pandemic - Are We Prepared?","authors":"Dr. Ashok Lingappa","doi":"10.12944/edj.03.01.01","DOIUrl":"https://doi.org/10.12944/edj.03.01.01","url":null,"abstract":"As per the published literature till September 2020, the only oral manifestations of the Covid infection are loss of smell and taste sensation. These could be due to the direct effects of tropism of virus to tongue and oral mucous membrane.2, 3 Lateron there were emergence of secondary infections and various oral manifestations varying from ulcers to devastating fungal infections like mucormycosis.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77405954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Masoudi Rad, H. Asli, M. Rabiei, M. Falahchai, F. Dds
Edentulism is one of the most important oral health challenges, which results in adverse social and psychological consequences. A conventional complete denture is utmost usually used treatment modality for these patients. A few studies assessed the quality of life of patients before as well as one month after complete denture therapy. This study aimed to assess the consequence of complete denture therapy on oral health-related quality of life of edentulous patients. This study was conducted on 43 edentulous patients in 2018-2019 who met the inclusion criteria. After obtaining their written informed consent and ethical approval from the university’s medical ethics committee, patients were requested to fill out the standardized Persian version of the oral health impact profile-14 (OHIP-14) questionnaire before as well as one month after the delivery of complete denture via an interview. Data were analyzed using SPSS version 21 via the Wilcoxon and MannWhitney U tests. The total score of quality of life (OHIP-14) of patients considerably improved after treatment (P<0.001). The quality of life of patients significantly improved in all domains (P<0.001) except for functional limitation (P=0.122) and physical pain (P=0.009) domains, which had a more favourable status before the denture delivery. The difference in OHIP-14 total score of patients was significant regarding sex and age before (P=0.004 and P=0.006, respectively) and after (P=0.022 and P=0.007, respectively) denture delivery. Edentulous patients showed an improvement in oral health related quality of life at one month after denture delivery.
蛀牙症是最重要的口腔健康挑战之一，它会导致不良的社会和心理后果。传统的全口义齿是这些患者最常用的治疗方式。一些研究评估了患者在全口义齿治疗前和治疗后一个月的生活质量。本研究旨在评估全口义齿治疗对无牙患者口腔健康相关生活质量的影响。本研究纳入2018-2019年符合纳入标准的43例无牙患者。在获得大学医学伦理委员会的书面知情同意和伦理批准后，患者通过访谈填写标准化波斯语版口腔健康影响概况-14 (OHIP-14)问卷，并在交付全口义齿之前和之后一个月填写。数据分析采用SPSS version 21，采用Wilcoxon和MannWhitney U检验。治疗后患者生活质量总分(OHIP-14)明显改善(P<0.001)。除功能受限(P=0.122)和躯体疼痛(P=0.009)外，患者的生活质量在义齿植入前均有显著改善(P<0.001)。患者在义齿植入前(P=0.004、P=0.006)和植入后(P=0.022、P=0.007) OHIP-14总分在性别、年龄上差异均有统计学意义。无牙患者在假牙放置后一个月的口腔健康相关生活质量有所改善。
{"title":"Effect of Complete Denture Therapy on Oral Health-Related Quality of Life of Edentulous Patient","authors":"H. Masoudi Rad, H. Asli, M. Rabiei, M. Falahchai, F. Dds","doi":"10.12944/edj.02.01.05","DOIUrl":"https://doi.org/10.12944/edj.02.01.05","url":null,"abstract":"Edentulism is one of the most important oral health challenges, which results in adverse social and psychological consequences. A conventional complete denture is utmost usually used treatment modality for these patients. A few studies assessed the quality of life of patients before as well as one month after complete denture therapy. This study aimed to assess the consequence of complete denture therapy on oral health-related quality of life of edentulous patients. This study was conducted on 43 edentulous patients in 2018-2019 who met the inclusion criteria. After obtaining their written informed consent and ethical approval from the university’s medical ethics committee, patients were requested to fill out the standardized Persian version of the oral health impact profile-14 (OHIP-14) questionnaire before as well as one month after the delivery of complete denture via an interview. Data were analyzed using SPSS version 21 via the Wilcoxon and MannWhitney U tests. The total score of quality of life (OHIP-14) of patients considerably improved after treatment (P<0.001). The quality of life of patients significantly improved in all domains (P<0.001) except for functional limitation (P=0.122) and physical pain (P=0.009) domains, which had a more favourable status before the denture delivery. The difference in OHIP-14 total score of patients was significant regarding sex and age before (P=0.004 and P=0.006, respectively) and after (P=0.022 and P=0.007, respectively) denture delivery. Edentulous patients showed an improvement in oral health related quality of life at one month after denture delivery.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75656838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Aoun, Ralph Zarazir, Rasha Hajj, Abillama Fadi, Esther Frei
Local and locoregional anesthesia (LRA) are extensively used for performing painless intraoral procedures. In rare cases, ophthalmic complications such as diplopia, blepharoptosis, mydriasis, etc., may occur. In general, symptoms disappear hours after the injection and rarely persist for a prolonged duration. The aim of this paper is to emphasize the ophthalmic complications that may occur after intraoral anesthesia injection in dentistry.Symptoms, management and prevention of the most common ophthalmic complications related to intraoral local and/or LRA anesthesia are reviewed. Despite being uncommon, ophthalmic complications may happen and lead to stressful situations. Dentists should be aware of their causes in order to prevent them.
{"title":"Ophthalmic Complications of Locoregional Anesthesia in Dentistry","authors":"G. Aoun, Ralph Zarazir, Rasha Hajj, Abillama Fadi, Esther Frei","doi":"10.12944/edj.02.01.02","DOIUrl":"https://doi.org/10.12944/edj.02.01.02","url":null,"abstract":"Local and locoregional anesthesia (LRA) are extensively used for performing painless intraoral procedures. In rare cases, ophthalmic complications such as diplopia, blepharoptosis, mydriasis, etc., may occur. In general, symptoms disappear hours after the injection and rarely persist for a prolonged duration. The aim of this paper is to emphasize the ophthalmic complications that may occur after intraoral anesthesia injection in dentistry.Symptoms, management and prevention of the most common ophthalmic complications related to intraoral local and/or LRA anesthesia are reviewed. Despite being uncommon, ophthalmic complications may happen and lead to stressful situations. Dentists should be aware of their causes in order to prevent them.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74430984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aminzadeh, Keivan Shirneshan, Nooshnaz Sabour, Mohammadreza Korang Beheshti
Adenomatoid hyperplasia is a rare lesion of the minor salivary glands recognized by localized swelling that mimics a neoplasm. This so-called pseudotumor usually occurring in adults 4th to 6th decade of life mostly, in 80%, occurs as a unilateral swelling without ulcer on the hard and soft palate. The pathogenesis of this condition is not well known but local trauma might have a role. In this case report three cases of adenomatoid hyperplasia have been reported to bring attention to this lesion as it is clinically similar to many soft tissue lesions of oral mucosa so should be considered while making a clinical diagnosis. Also, Pathologists should investigate a proper sample size under a microscope not to confuse a normal salivary gland adjacent to salivary gland tumours with Adenomatoid hyperplasia.
{"title":"Adenomatoid Hyperplasia: Case Report of a Rare Lesion in Uncommon Location","authors":"A. Aminzadeh, Keivan Shirneshan, Nooshnaz Sabour, Mohammadreza Korang Beheshti","doi":"10.12944/edj.02.01.08","DOIUrl":"https://doi.org/10.12944/edj.02.01.08","url":null,"abstract":"Adenomatoid hyperplasia is a rare lesion of the minor salivary glands recognized by localized swelling that mimics a neoplasm. This so-called pseudotumor usually occurring in adults 4th to 6th decade of life mostly, in 80%, occurs as a unilateral swelling without ulcer on the hard and soft palate. The pathogenesis of this condition is not well known but local trauma might have a role. In this case report three cases of adenomatoid hyperplasia have been reported to bring attention to this lesion as it is clinically similar to many soft tissue lesions of oral mucosa so should be considered while making a clinical diagnosis. Also, Pathologists should investigate a proper sample size under a microscope not to confuse a normal salivary gland adjacent to salivary gland tumours with Adenomatoid hyperplasia.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81822034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are many challenges and many barriers to the maxillofacial profession for female surgeons, particularly the beginners of their career. The reported analysis data revealed that this is not concentrated in developing countries, but it is unexpectedly found in many developed and high-income countries. The continued progress of women in academic surgery depends on addressing such issues and trying to solve these challenging problems.
{"title":"Maxillofacial Female Surgeons: Perspectives and Obstacles","authors":"Shadia A. Elsayed","doi":"10.12944/edj.02.01.07","DOIUrl":"https://doi.org/10.12944/edj.02.01.07","url":null,"abstract":"There are many challenges and many barriers to the maxillofacial profession for female surgeons, particularly the beginners of their career. The reported analysis data revealed that this is not concentrated in developing countries, but it is unexpectedly found in many developed and high-income countries. The continued progress of women in academic surgery depends on addressing such issues and trying to solve these challenging problems.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74520349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nagarajan, V. Rani, A. Narayanasamy, Mahaboob Kadar Masthan
This article aimed at discussing the effectiveness of developing a new antioxidant and anti-inflammatory product from the natural source namely the extract of Leucas aspera plant that can be used to treat various oral mucosal conditions. The study was a descriptive experimental study. The dried powder extract of the whole LA plant was subjected to various procedures such as phytochemical analysis, antioxidant assay (DPPH) and assessment of anti-inflammatory effect (Cytokine activity in RAW 264.7 macrophage cells) and the results were tabulated. Significance of anti-inflammatory activity was tested by T-test. Our analysis has confirmed that 100µg of plant extract showed high amount of antioxidant activity (78%). The cytokine inhibitory percentage of our plant extract showed consistent increase when tested from 250 ug/ml to 1000ug/ml to be from 24% to 39% thus demonstrating significantly high level of anti-inflammatory potential (P > 0.01). Our study suggests that LA plant can be safely utilized for testing and developing products based on its extract.
{"title":"Leucas aspera to Treat Pan Oral Disorders - An Experimental Study","authors":"S. Nagarajan, V. Rani, A. Narayanasamy, Mahaboob Kadar Masthan","doi":"10.12944/edj.01.01.05","DOIUrl":"https://doi.org/10.12944/edj.01.01.05","url":null,"abstract":"This article aimed at discussing the effectiveness of developing a new antioxidant and anti-inflammatory product from the natural source namely the extract of Leucas aspera plant that can be used to treat various oral mucosal conditions. The study was a descriptive experimental study. The dried powder extract of the whole LA plant was subjected to various procedures such as phytochemical analysis, antioxidant assay (DPPH) and assessment of anti-inflammatory effect (Cytokine activity in RAW 264.7 macrophage cells) and the results were tabulated. Significance of anti-inflammatory activity was tested by T-test. Our analysis has confirmed that 100µg of plant extract showed high amount of antioxidant activity (78%). The cytokine inhibitory percentage of our plant extract showed consistent increase when tested from 250 ug/ml to 1000ug/ml to be from 24% to 39% thus demonstrating significantly high level of anti-inflammatory potential (P > 0.01). Our study suggests that LA plant can be safely utilized for testing and developing products based on its extract.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83804285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Calcifying odontogenic cyst (COC) or calcifying cystic odontogenic tumor was first introduced in 1962 by Gorlin et al., as a possible oral counterpart of calcifying epitheliomas of Malherbe in skin. This lesion is a rare odontogenic lesion with variable clinico-histological characteristics. Three different histologic subtypes has been reported for COC. In this study we presented a female patient diagnosed with ameloblastomatous COC a very rare variant of this lesion and challenges regarding microscopic diagnosis and treatment of it is discussed.
{"title":"Ameloblastomatous Calcifying Odontogenic Cyst: Case Report","authors":"A. Aminzadeh, A. Sadighi","doi":"10.12944/cdrj.01.o1.02","DOIUrl":"https://doi.org/10.12944/cdrj.01.o1.02","url":null,"abstract":"Calcifying odontogenic cyst (COC) or calcifying cystic odontogenic tumor was first introduced in 1962 by Gorlin et al., as a possible oral counterpart of calcifying epitheliomas of Malherbe in skin. This lesion is a rare odontogenic lesion with variable clinico-histological characteristics. Three different histologic subtypes has been reported for COC. In this study we presented a female patient diagnosed with ameloblastomatous COC a very rare variant of this lesion and challenges regarding microscopic diagnosis and treatment of it is discussed.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76809895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The oral microbiome may be affected by patient medications, disease conditions and systemic disorders. Selenomonas noxia is an anaerobic, motile, non spore-forming, gram-negative rod that has been repeatedly associated with periodontal disease and other disorders, including obesity. Based upon the paucity of evidence regarding oral prevalence, the objective of was to evaluate S. noxia prevalence by sampling saliva from the oral cavity to screen for this pathogen. Using an existing saliva repository, DNA was isolated and screened using quantitative real-time polymerase chain reaction (qPCR). Demographic analysis of study samples and qPCR results was also performed. Approximately half of the samples (n=96) were derived from females (51%) and the majority were from Hispanic patients (62.5%). Following DNA isolation and qPCR screening 37.5% (n=35) were found to harbor S. noxia DNA, which was more prevalent among the samples derived from adults (n=22 or 22.9%) than pediatric patients (n=13 or 13.5%). This study provides novel information regarding the oral prevalence of S. noxia among both pediatric and adult populations from a dental school population. These data are an important part of the overall epidemiologic analysis of this organism and may provide some initial information regarding the risk for periodontal or other health issues related to the presence among these populations.
{"title":"Salivary screening for Selenomonas noxia in the oral cavity of pediatric patients","authors":"R. Davidowitz, K. Howard, K. Kingsley","doi":"10.12944/cdrj.01.o1.01","DOIUrl":"https://doi.org/10.12944/cdrj.01.o1.01","url":null,"abstract":"The oral microbiome may be affected by patient medications, disease conditions and systemic disorders. Selenomonas noxia is an anaerobic, motile, non spore-forming, gram-negative rod that has been repeatedly associated with periodontal disease and other disorders, including obesity. Based upon the paucity of evidence regarding oral prevalence, the objective of was to evaluate S. noxia prevalence by sampling saliva from the oral cavity to screen for this pathogen. Using an existing saliva repository, DNA was isolated and screened using quantitative real-time polymerase chain reaction (qPCR). Demographic analysis of study samples and qPCR results was also performed. Approximately half of the samples (n=96) were derived from females (51%) and the majority were from Hispanic patients (62.5%). Following DNA isolation and qPCR screening 37.5% (n=35) were found to harbor S. noxia DNA, which was more prevalent among the samples derived from adults (n=22 or 22.9%) than pediatric patients (n=13 or 13.5%). This study provides novel information regarding the oral prevalence of S. noxia among both pediatric and adult populations from a dental school population. These data are an important part of the overall epidemiologic analysis of this organism and may provide some initial information regarding the risk for periodontal or other health issues related to the presence among these populations.","PeriodicalId":11858,"journal":{"name":"ENVIRO Dental Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83901019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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