Mast Cells (MCs) have diverse roles in health and disease. These immune cells migrate in a tissue-specific manner to target sites where they can alert or amplify immune responses through exocytosis of their bioactive granules. Granule exocytosis can be triggered by both external immunological and non-immunological signals. Depending on the local milieu, MCs are highly heterogeneous cells with significant phenotypic plasticity and functional diversity. The aim of this study was to investigate differences in MC populations in the tongue and liver to advance the understanding of how organ-specific location may affect MC phenotypic variance and functions. MCs were investigated using light and electron microscopy in tissues obtained from rats housed in identical conditions. Connective tissue MCs were identified in both the tongue and liver and showed phenotypic differences in secretory activity and granule characteristics. These findings indicate that phenotypic variance depends on tissue location. This knowledge may help develop strategies for preventing or treating diseases.
肥大细胞（MC）在健康和疾病中发挥着多种作用。这些免疫细胞以组织特异性的方式迁移到目标部位，通过其生物活性颗粒的外渗出来提醒或扩大免疫反应。颗粒外渗可由外部免疫和非免疫信号触发。根据当地环境的不同，MCs 是高度异质的细胞，具有显著的表型可塑性和功能多样性。本研究旨在调查舌头和肝脏中 MC 群体的差异，从而进一步了解器官特异性位置如何影响 MC 的表型差异和功能。研究人员使用光镜和电子显微镜对在相同条件下饲养的大鼠组织中的 MCs 进行了研究。在舌头和肝脏中都发现了结缔组织 MCs，它们在分泌活性和颗粒特征方面表现出表型差异。这些发现表明，表型差异取决于组织位置。这些知识可能有助于制定预防或治疗疾病的策略。
{"title":"MAST CELLS IN THE MILIEU: A COMPARATIVE STUDY IN THE TONGUE AND LIVER","authors":"Y. Makeyeva, Steven T. Leach, D. Ryugo","doi":"10.25040/ntsh2023.02.10","DOIUrl":"https://doi.org/10.25040/ntsh2023.02.10","url":null,"abstract":"Mast Cells (MCs) have diverse roles in health and disease. These immune cells migrate in a tissue-specific manner to target sites where they can alert or amplify immune responses through exocytosis of their bioactive granules. Granule exocytosis can be triggered by both external immunological and non-immunological signals. Depending on the local milieu, MCs are highly heterogeneous cells with significant phenotypic plasticity and functional diversity. The aim of this study was to investigate differences in MC populations in the tongue and liver to advance the understanding of how organ-specific location may affect MC phenotypic variance and functions. MCs were investigated using light and electron microscopy in tissues obtained from rats housed in identical conditions. Connective tissue MCs were identified in both the tongue and liver and showed phenotypic differences in secretory activity and granule characteristics. These findings indicate that phenotypic variance depends on tissue location. This knowledge may help develop strategies for preventing or treating diseases.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"28 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139166177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dear readers! We will celebrate the Taras Shevchenko Scientific Society’s 150th anniversary in December. This is the first global Ukrainian union of scientists, the multidisciplinary Academy of Sciences of the Ukrainian people, which laid the foundation for shaping and developing Ukrainian science in the world, serving Ukraine and preserving national identity. In addition, February 15, 2023, marked the 125th anniversary of the creation of the Medical Commission and the publication of the “Medical Collection”, which published contributions from medical specialists in Europe and all of Ukraine. This was the first professional organization of physicians and the first edition of their scientific works that disseminated the achievements of Ukrainian medical scientists and practicing physicians globally and advocated for international recognition of Ukrainian medical science. The Ukrainian Medical Society, created in Lviv in 1910, played a significant role in developing a national union of physicians and public health professionals. These two organizations together created the foundations for international recognition of the Ukrainian nation in the field of medicine and its entry into global scientific discourse. Over centuries, Ukraine’s medical scientists and professionals actively represented and continue to advocate for their country worldwide, especially in recent years, which have been a great test for our nation and state. For nine years, Ukraine has endured the conditions of an imperialistic war and full-scale invasion of our land brought on by the Russian Federation. Thanks to the falsehoods and insidious propaganda of the Russian Federation, from 2014 until early 2022, the world interpreted events in Ukraine in muted colors. Ukraine, at all levels of society and various professional bodies, including medical facilities, warned of future military consequences. During this period, the Moscow-Ukraine war impacted the activities of medical and public health organizations in Ukraine. At all international events, Ukrainian medical scientists and doctors showcased their national professional organizations, set up appropriate displays, actively used Ukrainian symbols, and presented reports and scientific developments specifically focused on the consequences of war, such as military injuries and psychological problems that affect the health of citizens, especially children, and clearly laid the blame at the feet of the enemy – the Russian Federation, which occupied parts of the territory of Ukraine and destroyed peaceful lives of our citizens. Many conferences devoted to military medicine were held in Ukraine. But the world did not react and remained silent, including medical and scientific organizations. After February 24, 2022, civilized nations of this world realized that the destruction of an entire country and its people by the Russian Empire in the center of Europe is a moral challenge to democratic principles, and such a war can threaten the
{"title":"UKRAINE AND THE WORLD IN CONDITIONS OF WAR: THE ROLE OF SCIENTIFIC, PROFESSION AND PUBLIC ORGANIZATIONS","authors":"V. Chopyak, Wolodymyr P. Maksymowych","doi":"10.25040/ntsh2023.01.01","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.01","url":null,"abstract":"Dear readers! \u0000We will celebrate the Taras Shevchenko Scientific Society’s 150th anniversary in December. This is the first global Ukrainian union of scientists, the multidisciplinary Academy of Sciences of the Ukrainian people, which laid the foundation for shaping and developing Ukrainian science in the world, serving Ukraine and preserving national identity. In addition, February 15, 2023, marked the 125th anniversary of the creation of the Medical Commission and the publication of the “Medical Collection”, which published contributions from medical specialists in Europe and all of Ukraine. This was the first professional organization of physicians and the first edition of their scientific works that disseminated the achievements of Ukrainian medical scientists and practicing physicians globally and advocated for international recognition of Ukrainian medical science. The Ukrainian Medical Society, created in Lviv in 1910, played a significant role in developing a national union of physicians and public health professionals. These two organizations together created the foundations for international recognition of the Ukrainian nation in the field of medicine and its entry into global scientific discourse. Over centuries, Ukraine’s medical scientists and professionals actively represented and continue to advocate for their country worldwide, especially in recent years, which have been a great test for our nation and state. \u0000For nine years, Ukraine has endured the conditions of an imperialistic war and full-scale invasion of our land brought on by the Russian Federation. Thanks to the falsehoods and insidious propaganda of the Russian Federation, from 2014 until early 2022, the world interpreted events in Ukraine in muted colors. Ukraine, at all levels of society and various professional bodies, including medical facilities, warned of future military consequences. During this period, the Moscow-Ukraine war impacted the activities of medical and public health organizations in Ukraine. At all international events, Ukrainian medical scientists and doctors showcased their national professional organizations, set up appropriate displays, actively used Ukrainian symbols, and presented reports and scientific developments specifically focused on the consequences of war, such as military injuries and psychological problems that affect the health of citizens, especially children, and clearly laid the blame at the feet of the enemy – the Russian Federation, which occupied parts of the territory of Ukraine and destroyed peaceful lives of our citizens. \u0000Many conferences devoted to military medicine were held in Ukraine. But the world did not react and remained silent, including medical and scientific organizations. After February 24, 2022, civilized nations of this world realized that the destruction of an entire country and its people by the Russian Empire in the center of Europe is a moral challenge to democratic principles, and such a war can threaten the","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116735965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by fibrosis and vascular abnormalities. Despite extensive research, there is currently no effective treatment for SSc. This study aimed to investigate the effects of α-tocopherol acetate and vitamin D3 on the levels of surfactant protein D (SP-D), interleukin-13 (IL-13), and vascular cell adhesion molecule-1 (VCAM-1) in a preclinical model of SSc. The study included an intact group (IG) (15 animals) with no interventions, control group (CG) (20 animals) injected with isotonic solution, an experimental group #1 (EG#1) (25 animals) that were induced with SSc by injecting them subcutaneously with 0.5 ml of 5% (NaClO) three times a week for six consecutive weeks; and experimental group #2 (EG#2) (25 animals) with correction provided by injections of vitamin D (1000 IU / 100 g) and α-tocopherol acetate (10 mg / 100 g ) intramuscularly for 3 weeks. The serum concentrations of IL-13, SP-D, and VCAM-1 were significantly higher in the EG#1 compared to the control group (109.35 (93,23-199.05) vs 8.50 (5.60-14.20), p=0.004; 490.20 (156.20-605.70) vs 78.10 (40.80-100.40), p=0.004; 91.25 (85.00 -264.98) vs 19.50 (13.53-22.20), p=0.004 respectively). The administration of vitamin D3 and α-tocopherol acetate was found to have a positive effect on all three parameters investigated. The SP-D level in the EG#2 was significantly lower than that in the EG#1 (490.20 (156.20-605.70) vs 123.75 (108.80-145.03), p=0.004). The concentration of IL-13 and VCAM-1 were also lower in the EG#2. In conclusion, this study provides evidence of the beneficial effects of vitamin D3 and α-tocopherol acetate in reducing the levels of SP-D, IL-13, and VCAM-1 in a preclinical model of systemic sclerosis.
系统性硬化症(SSc)是一种以纤维化和血管异常为特征的慢性自身免疫性疾病。尽管进行了广泛的研究，但目前尚无有效的治疗SSc的方法。本研究旨在探讨α-生育酚醋酸酯和维生素D3对SSc临床前模型中表面活性蛋白D (SP-D)、白细胞介素-13 (IL-13)和血管细胞粘附分子-1 (VCAM-1)水平的影响。研究包括完整组(IG)(15只动物)不进行干预，对照组(CG)(20只动物)注射等渗溶液，实验组1 (EG#1)(25只动物)通过每周3次皮下注射0.5 ml 5% (NaClO)诱导SSc，连续6周;实验组2 (EG#2)(25只动物)通过肌肉注射维生素D (1000 IU / 100 g)和α-生育酚醋酸酯(10 mg / 100 g)进行校正，持续3周。EG#1患者血清IL-13、SP-D和VCAM-1浓度显著高于对照组(109.35 (93,23-199.05)vs 8.50 (5.60-14.20)， p=0.004;490.20 (156.20-605.70) vs 78.10 (40.80-100.40)， p=0.004;91.25 (85.00 -264.98) vs 19.50 (13.53-22.20)， p=0.004。维生素D3和α-生育酚醋酸酯对上述三个指标均有积极影响。EG#2的SP-D水平显著低于EG#1 (490.20 (156.20-605.70) vs 123.75 (108.80-145.03)， p=0.004)。EG#2中IL-13和VCAM-1的浓度也较低。总之，本研究提供了维生素D3和α-生育酚醋酸酯在降低系统性硬化症临床前模型中SP-D、IL-13和VCAM-1水平的有益作用的证据。
{"title":"VITAMIN D3 AND Α-TOCOPHEROL ACETATE AMELIORATE INFLAMMATORY AND FIBROTIC PROCESSES IN SYSTEMIC SCLEROSIS: PRECLINICAL EVIDENCE","authors":"B. Doskaliuk, L. Zaiats, Latika Gupta","doi":"10.25040/ntsh2023.01.09","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.09","url":null,"abstract":"Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by fibrosis and vascular abnormalities. Despite extensive research, there is currently no effective treatment for SSc. This study aimed to investigate the effects of α-tocopherol acetate and vitamin D3 on the levels of surfactant protein D (SP-D), interleukin-13 (IL-13), and vascular cell adhesion molecule-1 (VCAM-1) in a preclinical model of SSc. \u0000The study included an intact group (IG) (15 animals) with no interventions, control group (CG) (20 animals) injected with isotonic solution, an experimental group #1 (EG#1) (25 animals) that were induced with SSc by injecting them subcutaneously with 0.5 ml of 5% (NaClO) three times a week for six consecutive weeks; and experimental group #2 (EG#2) (25 animals) with correction provided by injections of vitamin D (1000 IU / 100 g) and α-tocopherol acetate (10 mg / 100 g ) intramuscularly for 3 weeks. \u0000The serum concentrations of IL-13, SP-D, and VCAM-1 were significantly higher in the EG#1 compared to the control group (109.35 (93,23-199.05) vs 8.50 (5.60-14.20), p=0.004; 490.20 (156.20-605.70) vs 78.10 (40.80-100.40), p=0.004; 91.25 (85.00 -264.98) vs 19.50 (13.53-22.20), p=0.004 respectively). The administration of vitamin D3 and α-tocopherol acetate was found to have a positive effect on all three parameters investigated. The SP-D level in the EG#2 was significantly lower than that in the EG#1 (490.20 (156.20-605.70) vs 123.75 (108.80-145.03), p=0.004). The concentration of IL-13 and VCAM-1 were also lower in the EG#2. \u0000In conclusion, this study provides evidence of the beneficial effects of vitamin D3 and α-tocopherol acetate in reducing the levels of SP-D, IL-13, and VCAM-1 in a preclinical model of systemic sclerosis.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123002669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Zubchenko, I. Kril, Olena Nadizhko, Volodymyr Gayevsky, I. Hayduchok, L. Mogylnytska
Background. The spread of post-traumatic stress disorder (PTSD) and overcoming its consequences, including immune-related disorders, is one of the critical issues requiring extensive study and resolution in practical medicine, particularly under present conditions in Ukraine. Materials and methods. The study group consisted of 79 (27.5%) patients with verified PTSD: 46 (58.2%) female and 33 (41.8%) male, with an average age of 38.7±7.2 years; a control group of 20 apparently healthy people was used. The National Institute of Mental Health (NIMH) American National Center for PTSD (2013) questionnaire was used to verify PTSD. In addition, history taking, clinical examination, general and biochemical laboratory tests, and statistical analysis were performed. Results. All patients with PTSD experienced clinical disorders and changes in laboratory indicators, with a probable increase in absolute and relative values of neutrophils and mononuclear cells, an increase in the levels of acute phase proteins, and activation of transaminases. In addition, these patients were characterized as immunocompromised patients with the potential to study immunological disorders. Conclusions. The results of the review of the scientific literature and the clinical and paraclinical manifestations that we found in patients with PTSD indicate the role of immune mechanisms in the development of this syndrome and necessitate expanding diagnostic measures among such patients with the different pathogenetic approach of their management.
{"title":"POST-TRAUMATIC STRESS DISORDER: СLINICAL AND LABORATORY CHANGES AND POTENTIAL FOR IMMUNE DISORDERS","authors":"S. Zubchenko, I. Kril, Olena Nadizhko, Volodymyr Gayevsky, I. Hayduchok, L. Mogylnytska","doi":"10.25040/ntsh2023.01.11","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.11","url":null,"abstract":"Background. The spread of post-traumatic stress disorder (PTSD) and overcoming its consequences, including immune-related disorders, is one of the critical issues requiring extensive study and resolution in practical medicine, particularly under present conditions in Ukraine. \u0000Materials and methods. The study group consisted of 79 (27.5%) patients with verified PTSD: 46 (58.2%) female and 33 (41.8%) male, with an average age of 38.7±7.2 years; a control group of 20 apparently healthy people was used. The National Institute of Mental Health (NIMH) American National Center for PTSD (2013) questionnaire was used to verify PTSD. In addition, history taking, clinical examination, general and biochemical laboratory tests, and statistical analysis were performed. \u0000Results. All patients with PTSD experienced clinical disorders and changes in laboratory indicators, with a probable increase in absolute and relative values of neutrophils and mononuclear cells, an increase in the levels of acute phase proteins, and activation of transaminases. In addition, these patients were characterized as immunocompromised patients with the potential to study immunological disorders. \u0000Conclusions. The results of the review of the scientific literature and the clinical and paraclinical manifestations that we found in patients with PTSD indicate the role of immune mechanisms in the development of this syndrome and necessitate expanding diagnostic measures among such patients with the different pathogenetic approach of their management.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"194-199 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130675211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The “Nautilus” publishing house was founded in 1997, initially for the publication of the Ukrainian edition of “Scientific American” under the name “World of Science.” Each issue consisted of ten articles of immeasurable value from various fields of science and discoveries. The journal had a successful publishing run of seven years. A large circle of translators and scientific editors gathered at this publishing house and expanded their activities, motivated by the mission statement: “First – translate the most famous world textbooks into Ukrainian, then – elevate our national authors to this level.” The first medical textbook to be translated and published was “Langman’s Medical Embryology.” As a result of a lucky encounter with Dr. Paul Dzul, an otolaryngologist from Detroit, with support from the American-Ukrainian Medical Foundation (AUMF), “Nautilus” published the two-volume “Dorland’s Illustrated Medical Dictionary,” first English-Ukrainian, then Ukrainian-English edition. The goal of AUMF was also to translate well-known medical English textbooks into Ukrainian. It was and is essential to present Western medical standards to Ukrainian medical workers and to compile and develop Ukrainian medical terminology. Next came the translation of Frank Netter’s “Atlas of Human Anatomy” (Ukrainian-Latin), “Molecular Cell Biology” by Alberts, “Physics for Universities” by Young and Friedman, and the “Illustrated Medical-Psychological Dictionary” in 3 volumes. Additionally, “Nautilus” established the “Media Analytical Group (MAG)” for the purpose of translating websites and resources of the Verkhovna Rada of Ukraine (Ukrainian Parliament) into English. The 4-volume edition of Taras Shevchenko’s “Kobzar” was an absolute gem of “Nautilus.” These and other exciting moments of publishing are described in this article.
{"title":"FANTASY STORIES OF \"NAUTILUS\" BOOKS OR WHERE DREAMS LEAD","authors":"A. Zavadka, V. Lonchyna","doi":"10.25040/ntsh2023.01.16","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.16","url":null,"abstract":"The “Nautilus” publishing house was founded in 1997, initially for the publication of the Ukrainian edition of “Scientific American” under the name “World of Science.” Each issue consisted of ten articles of immeasurable value from various fields of science and discoveries. The journal had a successful publishing run of seven years. A large circle of translators and scientific editors gathered at this publishing house and expanded their activities, motivated by the mission statement: “First – translate the most famous world textbooks into Ukrainian, then – elevate our national authors to this level.” \u0000The first medical textbook to be translated and published was “Langman’s Medical Embryology.” As a result of a lucky encounter with Dr. Paul Dzul, an otolaryngologist from Detroit, with support from the American-Ukrainian Medical Foundation (AUMF), “Nautilus” published the two-volume “Dorland’s Illustrated Medical Dictionary,” first English-Ukrainian, then Ukrainian-English edition. The goal of AUMF was also to translate well-known medical English textbooks into Ukrainian. It was and is essential to present Western medical standards to Ukrainian medical workers and to compile and develop Ukrainian medical terminology. \u0000Next came the translation of Frank Netter’s “Atlas of Human Anatomy” (Ukrainian-Latin), “Molecular Cell Biology” by Alberts, “Physics for Universities” by Young and Friedman, and the “Illustrated Medical-Psychological Dictionary” in 3 volumes. Additionally, “Nautilus” established the “Media Analytical Group (MAG)” for the purpose of translating websites and resources of the Verkhovna Rada of Ukraine (Ukrainian Parliament) into English. \u0000The 4-volume edition of Taras Shevchenko’s “Kobzar” was an absolute gem of “Nautilus.” These and other exciting moments of publishing are described in this article.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130703843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Decamethoxine (DCM®) is a substance from a group of quaternary ammonium compounds. According to literary sources, the antimicrobial effect is due to a mechanism that combines damage to the cell membrane of bacteria and lysis of their protoplasts, as well as changes the permeability of the microbial cell membrane, causing its destruction. DCM® demonstrates activity against gram-positive and gram-negative bacteria (staphylococci, streptococci, diphtheria and Escherichia coli, salmonella, proteus, klebsiella, shigella, pseudomonads, clostridia), some fungi (yeast fungi, mold fungi). There are also reports of the antiviral activity of this compound. The purpose of this study was to highlight the results of the current work of Ukrainian scientists and analyze the properties and broad aspects of DCM® antiseptic, as well as demonstrate their clinical case for its effectiveness in periodontal practice. The analysis of the results of various publications allowed us to evaluate the full range of DCM® properties in terms of its therapeutic potential in modern preclinical and clinical studies, particularly in periodontal practice.
{"title":"EFFECTIVENESS OF USING DOMESTIC QUATERNARY AMMONIUM ANTISEPTIC IN GENERAL MEDICINE AND DENTISTRY (MODERN VIEW AND CLINICAL CASE)","authors":"Yu. A. Bezhuk, Olesia Martovlos (Hodovana)","doi":"10.25040/ntsh2023.01.07","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.07","url":null,"abstract":"Decamethoxine (DCM®) is a substance from a group of quaternary ammonium compounds. According to literary sources, the antimicrobial effect is due to a mechanism that combines damage to the cell membrane of bacteria and lysis of their protoplasts, as well as changes the permeability of the microbial cell membrane, causing its destruction. DCM® demonstrates activity against gram-positive and gram-negative bacteria (staphylococci, streptococci, diphtheria and Escherichia coli, salmonella, proteus, klebsiella, shigella, pseudomonads, clostridia), some fungi (yeast fungi, mold fungi). There are also reports of the antiviral activity of this compound. The purpose of this study was to highlight the results of the current work of Ukrainian scientists and analyze the properties and broad aspects of DCM® antiseptic, as well as demonstrate their clinical case for its effectiveness in periodontal practice. The analysis of the results of various publications allowed us to evaluate the full range of DCM® properties in terms of its therapeutic potential in modern preclinical and clinical studies, particularly in periodontal practice.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116855432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lidiya Maryenko, T. Litovchenko, T. Nehrych, Vartanush Florikyan
The literature data of the last three decades on the problem of comorbidity of multiple sclerosis (MS) and epilepsy have been analyzed, such as issues of pathogenesis, clinical course, prognosis, and treatment of this dual pathology. Epileptic seizures occur in 2–3% to 5.9% of patients with MS, which is 3–6 times more common than in the general population. The incidence of epilepsy raises with increasing duration and severity of MS, with its progressive course, and also depends on the effect of drugs for the treatment of MS. There is no unanimity in the literature on the age and gender characteristics of the occurrence of epileptic seizures in MS. Probable mechanisms of MS comorbidity and epilepsy are analyzed. Data on certain common pathophysiology of MS and epilepsy and the concept according to which the model of epilepsy in MS is considered as a network disease are presented. Data on clinical manifestations and diagnosis of comorbid MS with epilepsy are presented. Epileptic seizures can occur at any stage of MS: before the clinical manifestations, at the onset of the disease, in the late stages, or can indicate exacerbation of MS. Types of epileptic seizures with a dual diagnosis (MS + epilepsy) are diverse. The majority of patients (up to 87.5%) have focal seizures (aware or unaware) or focal seizures to bilateral tonic-clonic, and a small share of patients have seizures of unknown origin. Most researchers believe that patients with MS and epilepsy have a more severe MS course and a worse long-term prognosis. The main directions of MS treatment and the impact of such treatment on the development of epileptic seizures are highlighted. Data on the effect of some disease-modifying drugs for the treatment of MS on the course of epilepsy and, on the other hand, on the impact of some antiseizure medications on the course of MS are presented. It is concluded that patients with MS have individual profiles and inter-individual variability of epileptogenicity. The principles of treatment of epileptic seizures/epilepsy in patients with MS are proposed.
{"title":"COMORBIDITY OF MULTIPLE SCLEROSIS AND EPILEPSY: MORE QUESTIONS OR ANSWERS?","authors":"Lidiya Maryenko, T. Litovchenko, T. Nehrych, Vartanush Florikyan","doi":"10.25040/ntsh2023.01.05","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.05","url":null,"abstract":"The literature data of the last three decades on the problem of comorbidity of multiple sclerosis (MS) and epilepsy have been analyzed, such as issues of pathogenesis, clinical course, prognosis, and treatment of this dual pathology. Epileptic seizures occur in 2–3% to 5.9% of patients with MS, which is 3–6 times more common than in the general population. The incidence of epilepsy raises with increasing duration and severity of MS, with its progressive course, and also depends on the effect of drugs for the treatment of MS. There is no unanimity in the literature on the age and gender characteristics of the occurrence of epileptic seizures in MS. Probable mechanisms of MS comorbidity and epilepsy are analyzed. Data on certain common pathophysiology of MS and epilepsy and the concept according to which the model of epilepsy in MS is considered as a network disease are presented. Data on clinical manifestations and diagnosis of comorbid MS with epilepsy are presented. Epileptic seizures can occur at any stage of MS: before the clinical manifestations, at the onset of the disease, in the late stages, or can indicate exacerbation of MS. Types of epileptic seizures with a dual diagnosis (MS + epilepsy) are diverse. The majority of patients (up to 87.5%) have focal seizures (aware or unaware) or focal seizures to bilateral tonic-clonic, and a small share of patients have seizures of unknown origin. Most researchers believe that patients with MS and epilepsy have a more severe MS course and a worse long-term prognosis. The main directions of MS treatment and the impact of such treatment on the development of epileptic seizures are highlighted. Data on the effect of some disease-modifying drugs for the treatment of MS on the course of epilepsy and, on the other hand, on the impact of some antiseizure medications on the course of MS are presented. It is concluded that patients with MS have individual profiles and inter-individual variability of epileptogenicity. The principles of treatment of epileptic seizures/epilepsy in patients with MS are proposed.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"157 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134104437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Hodovanyi, N. Chukhray, O. Martovlos, O. Klyuchivska, I. Ivasechko, R. Stoika
Introduction. A search continues for effective means which may reduce the overload of harmful factors, eliminate the inflammatory process, and reduce stress on the periodontal tissues during the active period of orthodontic treatment. We developed and patented the gel composition (GC) Benzidaflaziverdine prepared based on Proteflazid® (flavonoids) and benzydamine hydrochloride (BH) T-Sept® for the local treatment of the periodontal tissues in the form of a periodontal dressing in the orthodontic patients. The aim of this study was to evaluate the cytocompatibility of different combinations of components in gel composition based on flavonoid complex and benzydamine hydrochloride (Benzidaflaziverdine) used for the treatment of periodontal diseases in orthodontic patients. For this, mechanisms of their cytopathic and cytoprotective effects will be explored using cultured human and mouse cells. Methods. We studied the effect of different supplements used in GC Benzidaflaziverdine on the viability of pseudonormal human keratinocytes of the HaCaT line and mouse fibroblasts of the BALB-3T3 line, and mouse macrophages of the J774.2 line. Various methods of cell survival assessment were used: MTT-assay, staining of cells with fluorescent dyes Hoechst 33342 and Propidium iodide (PI), as well as a test for the genotoxic effects on cells (DNA comet assay). The antioxidant properties of the developed GC variants were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl), Merck (Dam-stadt, Germany), and DCFDA-H2 (2’,7’-dichlorodihydrofluorescein diacetate). Results. We demonstrated that the Sample containing gel base and BH in the form of a solution (Tantum Verde®) possessed weak prooxidant properties. While the Sample contained gel base, powdered BH (T-Sept®) and Sample containing gel base and powdered BH (T-Sept® and Proteflazid®) possessed pronounced antioxidant properties. Conclusions. Tests with DPPH and DCFDA dyes were used to confirm the hypothesis regarding the cytoprotective effect of the patented gel composition Benzidaflaziverdine for local application in the form of a periodontal bandage due to the antioxidant activity of the flavonoid complex, which reaches the maximum level at the 2nd hour of exposure. This gel composition can be recommended for use in clinical periodontology for medical support of orthodontic patients before and during the active phase of orthodontic treatment.
{"title":"STUDY OF CYTOCOMPATIBILITY OF DIFFERENT SUPPLEMENTS IN GEL COMPOSITIONS USED FOR THE TREATMENT OF PERIODONTAL DISEASES IN ORTHODONTIC PATIENTS VIA EXPLORATION OF MECHANISMS OF THEIR CYTOPATHIC AND CYTOPROTECTIVE EFFECTS IN CULTURED HUMAN AND MOUSE CELLS","authors":"O. Hodovanyi, N. Chukhray, O. Martovlos, O. Klyuchivska, I. Ivasechko, R. Stoika","doi":"10.25040/ntsh2023.01.08","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.08","url":null,"abstract":"Introduction. A search continues for effective means which may reduce the overload of harmful factors, eliminate the inflammatory process, and reduce stress on the periodontal tissues during the active period of orthodontic treatment. We developed and patented the gel composition (GC) Benzidaflaziverdine prepared based on Proteflazid® (flavonoids) and benzydamine hydrochloride (BH) T-Sept® for the local treatment of the periodontal tissues in the form of a periodontal dressing in the orthodontic patients. \u0000The aim of this study was to evaluate the cytocompatibility of different combinations of components in gel composition based on flavonoid complex and benzydamine hydrochloride (Benzidaflaziverdine) used for the treatment of periodontal diseases in orthodontic patients. For this, mechanisms of their cytopathic and cytoprotective effects will be explored using cultured human and mouse cells. \u0000Methods. We studied the effect of different supplements used in GC Benzidaflaziverdine on the viability of pseudonormal human keratinocytes of the HaCaT line and mouse fibroblasts of the BALB-3T3 line, and mouse macrophages of the J774.2 line. Various methods of cell survival assessment were used: MTT-assay, staining of cells with fluorescent dyes Hoechst 33342 and Propidium iodide (PI), as well as a test for the genotoxic effects on cells (DNA comet assay). The antioxidant properties of the developed GC variants were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl), Merck (Dam-stadt, Germany), and DCFDA-H2 (2’,7’-dichlorodihydrofluorescein diacetate). \u0000Results. We demonstrated that the Sample containing gel base and BH in the form of a solution (Tantum Verde®) possessed weak prooxidant properties. While the Sample contained gel base, powdered BH (T-Sept®) and Sample containing gel base and powdered BH (T-Sept® and Proteflazid®) possessed pronounced antioxidant properties. \u0000Conclusions. Tests with DPPH and DCFDA dyes were used to confirm the hypothesis regarding the cytoprotective effect of the patented gel composition Benzidaflaziverdine for local application in the form of a periodontal bandage due to the antioxidant activity of the flavonoid complex, which reaches the maximum level at the 2nd hour of exposure. This gel composition can be recommended for use in clinical periodontology for medical support of orthodontic patients before and during the active phase of orthodontic treatment.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133282344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anastasiia Bondarenko, V. Chopyak, Yu.S. Stepanovskyi, K. Lishchuk-Yakymovych, A. Hilfanova
On December 15–17, 2022, the Winter Specialized Immunology School, “Inborn Errors of Immunity,” was held in Uzhgorod of Zakarpattia Region with the support of Danylo Halytsky Lviv National Medical University, the International Interdisciplinary Association of Medical Professionals, the All-Ukrainian Association of Pediatric Immunology NGO, and the International European University. This school aims to analyze new approaches to the early diagnosis of primary/congenital disorders of the immune response in the context of the multidisciplinary approach, as well as to discuss the strategy of therapeutic tactics of such patients. The scientific event was held in the case marathon and direct communication formats. At the beginning of the event, a round table was held with the participation of regional experts in pediatric and adult immunology from different parts of Ukraine. More active cooperation with European colleagues and the creation of a national registry of immunodeficient patients with impaired antibody synthesis were noted. More than 20 speeches by clinical immunologists/pediatric immunologists were presented during two days of intensive work. During this school, results of Biopharma activities, a domestic producer of immunoglobulins, and plans for the future were presented in the field of domestic immunology development and diagnosis of patients with inborn errors of immunity. Discussions during the Winter School revealed the need to actively protect persons with congenital disorders of the immune system against infections by means of timely systematic seasonal vaccination, replacement immunoglobulin therapy, prophylactic antimicrobial therapy, and coordinated activities of specialists from various fields to provide immunological aid to patients in war conditions. At the end of the “Inborn Errors of Immunity” Winter School, organizers expressed confidence that most efforts and ideas highlighted during the professional meeting will be successfully implemented.
{"title":"WINTER SPECIALIZED IMMUNOLOGY SCHOOL, “INBORN ERRORS OF IMMUNITY”","authors":"Anastasiia Bondarenko, V. Chopyak, Yu.S. Stepanovskyi, K. Lishchuk-Yakymovych, A. Hilfanova","doi":"10.25040/ntsh2023.01.02","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.02","url":null,"abstract":"On December 15–17, 2022, the Winter Specialized Immunology School, “Inborn Errors of Immunity,” was held in Uzhgorod of Zakarpattia Region with the support of Danylo Halytsky Lviv National Medical University, the International Interdisciplinary Association of Medical Professionals, the All-Ukrainian Association of Pediatric Immunology NGO, and the International European University. This school aims to analyze new approaches to the early diagnosis of primary/congenital disorders of the immune response in the context of the multidisciplinary approach, as well as to discuss the strategy of therapeutic tactics of such patients. The scientific event was held in the case marathon and direct communication formats. At the beginning of the event, a round table was held with the participation of regional experts in pediatric and adult immunology from different parts of Ukraine. More active cooperation with European colleagues and the creation of a national registry of immunodeficient patients with impaired antibody synthesis were noted. More than 20 speeches by clinical immunologists/pediatric immunologists were presented during two days of intensive work. During this school, results of Biopharma activities, a domestic producer of immunoglobulins, and plans for the future were presented in the field of domestic immunology development and diagnosis of patients with inborn errors of immunity. Discussions during the Winter School revealed the need to actively protect persons with congenital disorders of the immune system against infections by means of timely systematic seasonal vaccination, replacement immunoglobulin therapy, prophylactic antimicrobial therapy, and coordinated activities of specialists from various fields to provide immunological aid to patients in war conditions. At the end of the “Inborn Errors of Immunity” Winter School, organizers expressed confidence that most efforts and ideas highlighted during the professional meeting will be successfully implemented.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122489704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Cherkas, Veleka Allen, S. Bascle, E. Kasireddy, Ren Chen Min, D. Pushkarna, M. Pourrahmat, Aymeric Mahieu
OBJECTIVES: To describe the humanistic and economic burden of informal caregivers of children, adolescents, and young adults newly diagnosed with T1D. METHODS: An SLR was conducted in MEDLINE®, Embase®, and PsycInfo® (search date: December 7, 2021) to identify clinical and observational studies assessing the humanistic burden experienced by informal caregivers (i.e., persons who provide unpaid care) of children, adolescents, and young adults aged 6 to 21 who were diagnosed with T1D within three months. RESULTS: Four prospective cohort studies and one randomized controlled trial (RCT) were included. Caregiver sample size ranged from 59 to 191. Duration of T1D was 2 to 6 weeks. Among parents of children aged ≤11 years, mothers had a moderate posttraumatic stress disorder symptom severity (mean score: 11.9; standard deviation [SD]: 7.4), while fathers experienced mild severity (8.4; SD: 6.2; p < 0.001; Posttraumatic Diagnostic Scale). Mothers experienced clinically significant symptoms (defined as score >5) of anxiety (mean score: 7.5), social dysfunction (7.5), and somatic symptoms (5.1; General Health Questionnaire [GHQ]-28). Fathers showed clinically significant anxiety (5.5) and social dysfunction (7.7; GHQ-28) only. Results from the RCT indicated a mean hypoglycemia fear score of 46.6 out of 108 at diagnosis and 34.9 after 14 weeks, with a higher score indicating greater fear (Hypoglycemia Fear Survey-Parents). Parents of children aged >11 years experienced clinically significant social dysfunction (mean score for mothers/fathers: 7.6/7.1) and anxiety (mothers: 5.4; GHQ-28). Parents experienced moderate to extreme diabetes-specific stress (defined as score ≥5; mean score: 6.3; SD: 1.9) at diagnosis, and no to moderate stress (4.5; SD: 2.1) at 3 months. None of the included studies reported on economic burden. CONCLUSIONS: This SLR highlights parents of children and young adults with newly diagnosed T1D experience caregiver burden, with a greater burden among mothers than fathers. Due to the paucity of evidence, further studies are warranted to better understand the humanistic burden among this group of informal caregivers.
{"title":"HUMANISTIC BURDEN OF INFORMAL CAREGIVERS OF CHILDREN AND YOUNG ADULTS WITH NEWLY DIAGNOSED TYPE 1 DIABETES (T1D): A SYSTEMATIC LITERATURE REVIEW (SLR)","authors":"A. Cherkas, Veleka Allen, S. Bascle, E. Kasireddy, Ren Chen Min, D. Pushkarna, M. Pourrahmat, Aymeric Mahieu","doi":"10.25040/ntsh2023.01.04","DOIUrl":"https://doi.org/10.25040/ntsh2023.01.04","url":null,"abstract":"OBJECTIVES: To describe the humanistic and economic burden of informal caregivers of children, adolescents, and young adults newly diagnosed with T1D. \u0000METHODS: An SLR was conducted in MEDLINE®, Embase®, and PsycInfo® (search date: December 7, 2021) to identify clinical and observational studies assessing the humanistic burden experienced by informal caregivers (i.e., persons who provide unpaid care) of children, adolescents, and young adults aged 6 to 21 who were diagnosed with T1D within three months. \u0000RESULTS: Four prospective cohort studies and one randomized controlled trial (RCT) were included. Caregiver sample size ranged from 59 to 191. Duration of T1D was 2 to 6 weeks. Among parents of children aged ≤11 years, mothers had a moderate posttraumatic stress disorder symptom severity (mean score: 11.9; standard deviation [SD]: 7.4), while fathers experienced mild severity (8.4; SD: 6.2; p < 0.001; Posttraumatic Diagnostic Scale). Mothers experienced clinically significant symptoms (defined as score >5) of anxiety (mean score: 7.5), social dysfunction (7.5), and somatic symptoms (5.1; General Health Questionnaire [GHQ]-28). Fathers showed clinically significant anxiety (5.5) and social dysfunction (7.7; GHQ-28) only. Results from the RCT indicated a mean hypoglycemia fear score of 46.6 out of 108 at diagnosis and 34.9 after 14 weeks, with a higher score indicating greater fear (Hypoglycemia Fear Survey-Parents). Parents of children aged >11 years experienced clinically significant social dysfunction (mean score for mothers/fathers: 7.6/7.1) and anxiety (mothers: 5.4; GHQ-28). Parents experienced moderate to extreme diabetes-specific stress (defined as score ≥5; mean score: 6.3; SD: 1.9) at diagnosis, and no to moderate stress (4.5; SD: 2.1) at 3 months. None of the included studies reported on economic burden. \u0000CONCLUSIONS: This SLR highlights parents of children and young adults with newly diagnosed T1D experience caregiver burden, with a greater burden among mothers than fathers. Due to the paucity of evidence, further studies are warranted to better understand the humanistic burden among this group of informal caregivers.","PeriodicalId":345961,"journal":{"name":"Proceeding of the Shevchenko Scientific Society. Medical Sciences","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125799776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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