Zhonghua zheng xing shao shang wai ke za zhi = Zhonghua zheng xing shao shang waikf [i.e. waike] zazhi = Chinese journal of plastic surgery and burns最新文献

Objective: The experiment was to investigate the role of the pedicle of the subdermal vascular network skin flap.

Methods: Ten healthy white pigs, weighting from 20 to 25 kg were used. The subdermal vascular network skin flap and the traditional skin flap were designed and created on each side of the animal's flank. The traditional skin flap on the contralateral side of the flank was used as control. Flap survival was studied by gross observation and ECT examination of isotope distribution.

Results: There were significant differences between the two groups in mean survival length and area (P < 0.05). In both groups the radioactive isotope gathering is restricted proximally with a ratio of length to width of (1-1.5):1.

Conclusion: The presence of the pedicle of the subdermal vascular network skin flap makes no difference compared the traditional skin flap.

Effects of local use of sulfadiazine (SD) zinc, SD silver in the wound and skin transplantation on the combined injury of 6 Gy radiation and 15% TBSA burn were investigated in the present study. In the three groups of SD zinc or SD silver plus skin grafting at 15 d after combined injury and SD zinc alone, the 15-day survival rates were 63%, 69% and 75% respectively, and significantly higher than that of control group (42%). The repair time of burn wound was shorter and the number of white blood cells was lower than that of control. Animals in the group of local use of SD silver followed by skin grafting on the 7th day all died, but all of the animals survived when the skin grafting was performed after 15 days. These results suggested that SD zinc and SD silver showed some therapeutic effects on the combined radiation-burn injury. In critical phase of the combined injury, surgical operation should be prohibited. However, in the recovery phase, skin transplantation could be performed.

The study was designed to examine the amount and the direction of craniofacial growth changes following bilateral coronal sutures (BCS) closure in young rabbits. At 2 weeks of age, the BCS were fused by the application of enamel adhesive. These animals and a control group also received amalgam implants on the frontonasal, coronal, and lambdoid sutures to monitor suture growth. At 4 or 8 weeks after suture fusion, separate groups of animals underwent release of fused sutures. Immobilization of BCS resulted in growth cessation of the coronal suture. The normal sutures underwent very rapid growth from 2 to 8 weeks of age. BCS release at 8 weeks after fusion resulted in growth restriction. Significant compensatory growth was also noted in the frontal and the lambdoid sutures from 2 to 8 weeks. Nasal bone length was increased significantly. Vault length and craniofacial length were reduced. The results of the present study give experimental documentation to emphasize that surgical correction of craniosynostosis should be performed at a young age to achieve better functional and cosmetic results.

This study was to investigate the expressions of CD11a/CD18 and CD11b/CD18 on burn patient's polymorphonuclear neutrophil (PMN) in early postburn stage and effects of CD11/CD18 mAb on PMN-EC adhesion and endothelial cell (EC) damage induced by PMN. Adhesive rate between PMN and EC, the filtration coefficient (kf) and monolayer volume flux (Jv) were determined after incubating burn patient's PMN with human umbilical vein endothelial cell (HUVEC) for 24 hours. CD11a/CD18 monoclonal antibodies (mAb) and CD11b/CD18 mAb were used to pretreat burn patient's PMNs in order to demonstrate the role of CD11/CD18 in PMN-EC adhesion and EC damage. Expressions of CD11a/CD18 and CD11b/CD18 were determined by flow cytometry 1, 3, 5, 7 d postburn. The results indicated that expressions of CD11a/CD18 and CD11b/CD18 on burn patient's PMNs increased quickly, reaching the peak on day 1 postburn and remained in high level till 7 days postburn. The values of Kf and Jv were increased markedly after burn patient's PMNs was incubated with HUVECs, which were cultured on micropore filter membrane. When burn patient's PMNs were pretreated with CD11a/CD18 mAb and CD11b/CD18 mAb, PMN-HUVEC adhesive rate was inhibited by 70%-80% and the values of Jv and kf were decreased significantly. It suggested that there were increased expressions of CD11a/CD18 and CD11b/CD18 on PMNs after burn injury. Burn patient's PMNs could damage EC and increased EC monolayer permeability (kf, Jv). CD11/CD18 mAb inhibited PMN-EC adhesion and attenuated EC damage induced by PMNs.

Based on the character of degeneration and change of the aging skin, reconstruction and repair of the skin structure are performed with a method of subdermal incision in order to increase the content of collagen protein and replace the degenerates collagen in the dermis. The animal experiment shows that the treated skin contracts by nearly thirty percent (P < 0.01) at 15 days after surgery. The epidermis becomes thicker and the dermis contains more fiberoblasts and collagen than that of preoperation. Seven patients were treated with the method. Afterwards their crow's feet deformity and the wrinkles of the nasolabial groove almost disappeared.

The segmented-fibula osteoseptocutaneous flap has been used to repair the bone and soft tissue defects of the forearm. During the operation, the fibula is divided subperiosteally at proper site according to the bony defects. The two segments of the fibula were then inserted to the radial and ulnar defects before vascular anastomosis is performed. Experience shows that anastomosis of only one group of vessels can ensure survival of both the segmented fibula and the cutaneous flap. Care should be taken to protect the muscle cuff and periosteum of the fibula, which is the source of blood supply to one segment. Four patients with bone and soft tissue defects of the forearm have been treated using this method. All the flaps survived with good function. The success of this technique contributes to the application of the free fibular flap in repair of forearm injury or osteomyelitis.

Investigating spatial and temporal expression and distribution of endogenous TGF alpha, and clarifying its role in burn wound healing. Immunohistochemical and mRNA dot blot hybridization approaches were adopted. Thermal injury can induce TGF alpha gene expression. The expression peaks at the time when repair cells migrate and proliferate most actively, and declines on near completion of epithelialization. Otherwise, the mRNA expression of EGF receptor manifests the same change as TGF alpha's. Immunohistochemical investigation indicates that TGF alpha is mainly distributed in the survived hair follicles and reepithelialized cell layers, and the immunoreactive staining is proportional to epidermal cell growth and migration. Thermal injury can induce endogenous TGF alpha and EGF receptor expression, which is well controlled and correlated with the migration and proliferation of keratinocytes. Endogenous TGF alpha plays a positive role in regulation of wound healing.

The purpose of this experiment was to observe the effect of PGI2 on the survival of the random skin flap. White rats used in the experiment were divided into preoperation (prevention) group, postoperation (treatment) group and the control group. A super-long random skin flap was created on the back of the animal. Postoperatively, the treatment group received intravenous injection of PGI2 while the control group received intravenous injection of normal saline. The blood supply, metabolism and hemorheology of the flap were analyzed. Results showed that the relative blood flow on the distal part of the flap was 41.3% in the treatment group and 22.4% in the control group. The survival rate of the flap of the treatment group was higher than that of the control. There were no differences between the prevention and the control groups. It is concluded that early postoperative injection of PGI2 could improve blood supply and rheology of the flap and increase its survival rate.

In our previous study, a haptoglobin-like substance possessing immunosuppressive activity was found in the sera of burned patients. However, its properties have not yet been clarified. In the present study, we mainly investigated the properties of the haptoglobin-like substance. The results showed that the abnormal protein bands identified by SDS-PAGE were the different subunits of haptoglobin, i.e., beta, alpha 2 and alpha 1 chains. The haptoglobin in burned sera was identical to that in normal sera in molecular weight, isoelectric point, hemoglobin-binding capacity and immunochemical properties as analyzed by SDS-PAGE, isoelectric focusing, immunochemical and biochemical methods. According to our results, it is suggested that the haptoglobin-like substance reported in the past is probably the haptoglobin, and that there may be no alteration in the properties of haptoglobin following burns.