日常健康检查中的噪音、振动和光干扰对小鼠繁殖性能、筑巢和皮质酮的影响。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-07-05 Epub Date: 2023-06-20 DOI:10.30802/AALAS-JAALAS-23-000002
Bridget M Clancy, Betty R Theriault, Rebecca Turcios, George P Langan, Kerith R Luchins
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引用次数: 0

摘要

在我们的机构,技术人员用于健康检查的技术对单独通风的笼(IVC)架上的笼子中的老鼠有所不同。如果不能充分看到老鼠,一些技术人员会部分打开笼子,而另一些则使用LED手电筒。这些行为无疑会改变笼子的微环境,尤其是噪音、振动和光线,这些已知会影响小鼠的多种福利和研究相关参数。本研究的主要目的是评估在日常健康检查期间部分拆笼和使用LED手电筒对C57BL/6J小鼠的繁殖力、筑巢分数和毛发皮质酮浓度的影响,以确定进行这些健康检查的干扰最小的方法。此外,我们使用一个加速度计、一个麦克风和一个测光计来测量每个条件下的室内噪音、振动和光线。繁殖对(n = 100对)随机分为3个健康检查组:部分脱舱组、LED手电筒组和对照组(在不操纵笼子的情况下观察小鼠)。我们假设,在日常健康检查中暴露在手电筒或打开笼子的老鼠会比对照组的老鼠有更少的幼崽,更差的筑巢分数和更高的毛皮质酮水平。我们发现实验组与对照组相比,在繁殖力、筑巢得分或毛发皮质酮水平上没有统计学上的显著差异。然而,毛发皮质酮水平受到架子上笼子高度和研究时间的显著影响。这些结果表明,在C57BL/6J小鼠的巢得分和毛发皮质酮水平测量中,在日常健康检查期间,短时间、每天一次暴露于部分打开笼子或LED手电筒下,不会影响繁殖性能或健康。
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The Effect of Noise, Vibration, and Light Disturbances from Daily Health Checks on Breeding Performance, Nest Building, and Corticosterone in Mice.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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