在体外培养8天的牛卵母细胞中，预成熟培养可促进其发育能力。

IF 1.9 4区生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Reproduction and Development Pub Date : 2023-08-11 DOI:10.1262/jrd.2023-022

Madalitso Chelenga, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano

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引用次数: 0

摘要

在这项研究中，我们评估了预成熟培养(预ivm)对牛卵母细胞发育能力的影响，这些卵母细胞来自8天的体外生长(IVG)培养系统。IVG卵母细胞在体外成熟前进行5h的预ivm，然后进行体外受精(IVF)。预ivm组和未预ivm组的卵母细胞进入生发囊泡破裂期的比例相似。尽管体外受精后中期卵母细胞和卵裂率与体外受精前后无明显差异，但体外受精组的囊胚率(22.5%)显著高于未体外受精组(11.0%，P < 0.05)。综上所述，体外培养可提高体外培养8天的牛卵母细胞的发育能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system.

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.

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来源期刊

Journal of Reproduction and Development 生物-奶制品与动物科学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

2 months

期刊介绍： Journal of Reproduction and Development (JRD) is the official journal of the Society for Reproduction and Development, published bimonthly, and welcomes original articles. JRD provides free full-text access of all the published articles on the web. The functions of the journal are managed by Editorial Board Members, such as the Editor-in-Chief, Co-Editor-inChief, Managing Editors and Editors. All manuscripts are peer-reviewed critically by two or more reviewers. Acceptance is based on scientific content and presentation of the materials. The Editors select reviewers and correspond with authors. Final decisions about acceptance or rejection of manuscripts are made by the Editor-in-Chief and Co-Editor-in-Chief.