紫锥菊水醇提取物电喷涂纳米颗粒Moench通过增加雄性Wistar大鼠的炎症因子刺激免疫系统。

IF 3.1 Q2 PHARMACOLOGY & PHARMACY Advanced pharmaceutical bulletin Pub Date : 2023-03-01 DOI:10.34172/apb.2023.031
Fatemeh Mehdizadeh, Ramin Mohammadzadeh, Hossein Nazemiyeh, Mehran Mesgari-Abbasi, Mohammad Barzegar-Jalali, Morteza Eskandani, Khosro Adibkia
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引用次数: 0

摘要

目的:紫锥菊(L.)牡丹是一种菊科植物,传统上主要是由于其免疫刺激特性而被使用。各种化合物包括烷基酰胺和菊苣酸被报道为紫荆的有效成分。本实验以紫荆草RS100 (EP-Eudragit RS100 NPs)为材料,制备含有紫荆草水醇提取物的电喷涂纳米颗粒(NPs),以提高紫荆草水醇提取物的免疫调节作用。方法:采用电喷雾技术制备不同萃取物与聚合物比例和溶液浓度的EP-Eudragit RS100 NPs。采用动态光散射(DLS)和场发射扫描电镜(FE-SEM)对NPs的大小和形貌进行了评价。为了评价免疫应答,雄性Wistar大鼠分别以30或100 mg/kg的终剂量给药制备的EP-Eudragit RS100 NPs和纯提取物。采集动物血液,检测炎症因子和全血细胞计数(CBC)。结果:体内研究表明,与对照组相比,平提物和EP-Eudragit RS100 NPs (100 mg/kg)显著提高了血清肿瘤坏死因子-α (TNF-α)和白细胞介素1-β (il - 1-β)水平,EP-Eudragit RS100 NPs (30 mg/kg)显著增加了白细胞(wbc)数量。与对照组相比,各组大鼠淋巴细胞计数均显著升高(p)。结论:电喷雾技术制备的EP-Eudragit RS100 NPs可显著增强紫荆提取物的免疫刺激作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Electrosprayed Nanoparticles Containing Hydroalcoholic Extract of Echinacea purpurea (L.) Moench Stimulates Immune System by Increasing Inflammatory Factors in Male Wistar Rats.

Purpose: Echinacea purpurea (L.) Moench is a member of the Asteraceae family and is traditionally used mainly due to its immunostimulatory properties. Various compounds including alkylamides and chicoric acid were reported as active ingredients of E. purpurea. Here, we aimed to prepare electrosprayed nanoparticles (NPs) containing hydroalcoholic extract of E. purpurea using Eudragit RS100 (EP-Eudragit RS100 NPs) to improve the immunomodulatory effects of the extract. Methods: The EP-Eudragit RS100 NPs with the different extract:polymer ratios and solution concentrations were prepared using the electrospray technique. The size and morphology of the NPs were evaluated using dynamic light scattering (DLS) and field emission-scanning electron microscopy (FE-SEM). To evaluate the immune responses, male Wistar rats were administrated with the prepared EP-Eudragit RS100 NPs and plain extract in the final dose of 30 or 100 mg/kg. The blood samples of the animals were collected and the inflammatory factors and complete blood count (CBC) were investigated. Results: In vivo studies indicated that the plain extract and EP-Eudragit RS100 NPs (100 mg/kg) significantly increased the serum level of tumor necrosis factor-α (TNF-α) and interleukin 1-β (IL1-β) whereas the EP-Eudragit RS100 NPs (30 mg/kg) significantly increased the number of white blood cells (WBCs) compared to the control group. Lymphocytes' count in all groups was increased significantly compared to the control group (P<0.05) whereas other CBC parameters remained unchanged. Conclusion: The prepared EP-Eudragit RS100 NPs by electrospray technique caused significant reinforcement in the immunostimulatory effects of the extract of E. purpurea.

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来源期刊
Advanced pharmaceutical bulletin
Advanced pharmaceutical bulletin PHARMACOLOGY & PHARMACY-
CiteScore
6.80
自引率
2.80%
发文量
51
审稿时长
12 weeks
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