ChREBP 基因甲基化与 2 型糖尿病病理特征的相关性

IF 3.1 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2023-08-24 DOI:10.1007/s12010-023-04714-4
Xin Wang, Weizhong Huangfu, Feng Zhao
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引用次数: 0

摘要

本研究旨在探讨碳水化合物反应元件结合蛋白(ChREBP)基因在2型糖尿病(T2DM)中的表达及其与病理特征的相关性。为获取并探究患者的病理特征,选取2019年1月至2019年6月期间在我院就诊的60名T2DM患者(研究组)和30名健康对照者(对照组)作为研究对象。从外周血单核细胞(PBMCs)中提取DNA并用亚硫酸氢盐修饰靶基因甲基化后,验证甲基化差异,探讨ChREBP甲基化水平与T2DM病理特征及单核苷酸多态性(SNP)分型的相关性。根据 UCSC Genome Browser Home 的预测结果,ChREBP 基因启动子区有两个 CpG 岛,研究选择了第一个外显子。与对照组相比,研究组的 ChREBP 甲基化率在统计学上更高(P 0.05)。外周血 ChREBP 甲基化在 T2DM 患者中升高,且与年龄、血糖和血脂水平密切相关,有望成为未来 T2DM 诊断和治疗的新方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus
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来源期刊
Applied Biochemistry and Biotechnology
Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学
CiteScore
5.70
自引率
6.70%
发文量
460
审稿时长
5.3 months
期刊介绍: This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.
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