一种新的策略,以实现高抗菌活性:绿色合成银纳米颗粒配方与银镓和蜡菊。

IF 1.7 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology Pub Date : 2023-06-01 DOI:10.2478/aiht-2023-74-3684
Cilem Ozdemir, Merve Gencer, Irem Coksu, Tulin Ozbek, Serap Derman
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引用次数: 0

摘要

银纳米颗粒(AgNPs)也可以通过绿色合成来生产,近年来由于其抗菌活性而受到关注。本研究的目的是:(i)利用微波辅助的肝素镓(G-AgNPs)和蜡菊(H-AgNPs)的水提取物来表征绿色合成的AgNPs, (ii)研究不同比例的G-和H-AgNPs的联合抗菌效果。用紫外-可见光谱法测定纳米颗粒的形成和反应。G-AgNPs的尺寸为52.0±10.9 nm, PDI为0.285±0.034,zeta电位为-17.9±0.9 mV。对于H-AgNPs,这些特性分别为23.9±1.0 nm, 0.280±0.032和-21.3±2.7 mV。原子力显微镜(AFM)和扫描电镜(SEM)证实颗粒呈单分散球形。傅里叶变换红外光谱(FT-IR)结果表明,还原剂的存在稳定了AgNPs。将这两种合成的纳米颗粒以不同的比例组合,制备了三种不同的纳米制剂(NF-1、NF-2和NF-3),并测试了它们对大肠杆菌、金黄色葡萄球菌、白色念珠菌和黄芽孢杆菌的抗菌活性。我们的研究首次表明,结合来自两种不同生物来源的AgNPs可以产生有效的纳米配方,提高对大肠杆菌和金黄色葡萄球菌的抗菌活性。这些纳米制剂对大肠杆菌具有较低的最低抑制浓度(31.25µg/mL);对金黄色葡萄球菌,NF-1和NF-2/3分别为62.5µg/mL和125µg/mL),比单独使用g - agnps(对大肠杆菌为62.5µg/mL)或H-AgNPs(对金黄色葡萄球菌为125µg/mL)效果好。它们对大肠杆菌(NF-1-3)具有协同抑制作用,对金黄色葡萄球菌(NF-2和NF-3)具有潜在的相加抑制作用。考虑到这些有希望的结果,我们相信我们的研究为抗菌治疗的新研究和新策略提供了一些方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A new strategy to achieve high antimicrobial activity: green synthesised silver nanoparticle formulations with Galium aparine and Helichrysum arenarium.

Silver nanoparticles (AgNPs), which have recently gained attention due to their antimicrobial activity, can also be produced by green synthesis. The aims of this study were to (i) characterise green synthesized AgNPs using microwave-assisted aqueous extracts of Galium aparine (G-AgNPs) and Helichrysum arenarium (H-AgNPs) and (ii) investigate the combined antimicrobial effects of the G- and H-AgNPs in different ratios. Nanoparticle formation and reactions were determined with UV-Vis spectroscopy. The G-AgNPs were 52.0±10.9 nm in size, with a 0.285±0.034 polydispersity index (PDI), and a -17.9±0.9 mV zeta potential. For H-AgNPs these characteristics were 23.9±1.0 nm, 0.280±0.032, and -21.3±2.7 mV, respectively. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) confirmed that the particles were monodisperse and spherical. The Fourier transform-infrared spectroscopy (FT-IR) results showed the presence of reducing agents that stabilised the AgNPs. Three different nanoformulations (NF-1, NF-2, and NF-3) were prepared by combining these two synthesised nanoparticles in different ratios and their antimicrobial activity was tested against E. coli, S. aureus, C. albicans, and A. flavus. Our study is the first to show that combining AgNPs from two different biological sources can produce effective nanoformulations with improved antibacterial activity against E. coli and S. aureus. These nanoformulations showed lower minimum inhibitory concentrations (31.25 µg/mL against E. coli with all NFs; 62.5 µg/mL for NF-1 and 125 µg/mL for NF-2/3 against S. aureus) than G-AgNPs (62.5 µg/mL for E. coli) or H-AgNPs (125 µg/mL for S. aureus) alone. Their high combined inhibitory effect against E. coli (NF-1-3) was synergistic and against S. aureus (NF-2 and NF-3) potentially additive. Considering such promising results, we believe our study provides some direction for new research and strategies in antimicrobial therapeutics.

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来源期刊
Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-TOXICOLOGY
CiteScore
3.50
自引率
4.80%
发文量
26
审稿时长
6-12 weeks
期刊介绍: Archives of Industrial Hygiene and Toxicology (abbr. Arh Hig Rada Toksikol) is a peer-reviewed biomedical scientific quarterly that publishes contributions relevant to all aspects of environmental and occupational health and toxicology.
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