miR-122/miR-20a比值(miR-122/miR-20a)是检测非酒精性脂肪肝的一种有用的微创生物标志物。

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY Genetic testing and molecular biomarkers Pub Date : 2023-08-01 DOI:10.1089/gtmb.2022.0155
Yuji Hattori, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Yoshitaka Ando, Mirai Yamazaki, Genki Mizuno, Yoshiki Tsuboi, Yuya Ishihara, Naohiro Ichino, Keiko Sugimoto, Keisuke Osakabe, Hiroaki Ishikawa, Koji Ohashi, Koji Suzuki
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引用次数: 0

摘要

背景:非酒精性脂肪性肝病(NAFLD)的日益流行已成为一个全球性的健康问题。NAFLD的初始症状很少,可能难以早期发现,因此需要微创早期检测标志物。我们假设miR-122和miR-20a水平结合,因为miR-122/miR-20a比值可能更敏感地检测NAFLD。方法:本研究涉及167名低酒精摄入量的参与者。将超声显示肝实质回声增强及肝肾对比增强者分为NAFLD组(n = 44),根据回声强度及肝血管、膈可见性进一步分为轻度组(n = 26)和重度组(n = 18)。除体重指数、糖化血红蛋白和收缩压(n = 123)值异常的参与者外,无脂肪肝的参与者被纳入正常组。通过实时聚合酶链反应测定受试者血清miR-122和miR-20a的表达水平,并计算miR-122/miR-20a。结果:NAFLD组miR-122表达明显高于正常组,miR-20a表达明显低于正常组,与既往研究一致。NAFLD组miR-122/miR-20a水平也显著升高。以miR-122/miR-20a作为NAFLD检测标记进行受试者工作特征曲线分析,miR-122/miR-20a的曲线下面积明显大于单独使用miR-122或miR-20a。结论:miR-122/miR-20a比值,结合miR-122和miR-20a水平,是检测NAFLD的一种有用的生物标志物,灵敏度高。
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The Ratio of miR-122 to miR-20a (miR-122/miR-20a) Is a Useful Minimally Invasive Biomarker for Non-Alcoholic Fatty Liver Disease Detection.

Background: The increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has become a global health problem. NAFLD has few initial symptoms and may be difficult to detect early, so there is need for a minimally invasive early detection marker. We hypothesized that miR-122 and miR-20a levels combined, as the miR-122/miR-20a ratio might detect NAFLD more sensitively. Methods: This study involved 167 participants with low alcohol intake. Those who had an increase in echogenicity of the liver parenchyma and hepato-renal contrast on ultrasonography were classified as the NAFLD group (n = 44), which was further classified into mild (n = 26) and severe (n = 18) groups based on echogenic intensity and hepatic vessel and diaphragm visualization. Participants without fatty liver were included in the normal group, except for those with an abnormal body mass index, glycated hemoglobin, and systolic blood pressure (n = 123) values. Serum miR-122 and miR-20a expression levels in participants were measured by real-time polymerase chain reaction, and the miR-122/miR-20a was calculated. Results: In the NAFLD group, miR-122 expression was significantly higher and the miR-20a was significantly lower than in the normal group, in agreement with previous studies. miR-122/miR-20a was also significantly higher in the NAFLD group. Receiver operating characteristic curve analysis was performed with miR-122/miR-20a as an NAFLD detection marker, and the area under the curve of miR-122/miR-20a was significantly larger than that of miR-122 or miR-20a alone. Conclusions: The miR-122/miR-20a ratio, combined with miR-122 and miR-20a levels, is a useful biomarker to detect NAFLD with high sensitivity.

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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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