载氧微泡介导的超声灌注和氧合对缺血性脑卒中再灌注后神经保护的作用。

IF 11.3 1区 医学 Q1 Medicine Biomaterials Research Pub Date : 2023-07-06 DOI:10.1186/s40824-023-00400-y
Yi-Ju Ho, Hsiang-Lung Cheng, Lun-De Liao, Yu-Chun Lin, Hong-Chieh Tsai, Chih-Kuang Yeh
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引用次数: 1

摘要

背景:缺血性脑卒中再灌注(S/R)损伤是溶栓后脑功能保护的关键问题。超声刺激微泡空化引起的血管舒张已被应用于超声灌注减轻S/R损伤。本研究使用载氧微泡(OMBs)与US刺激提供超声灌注和局部氧治疗,以减少脑梗死面积和S/R后的神经保护。方法:采用光动力血栓和脑前动脉远端支溶栓法建立小鼠S/R模型。检测动物体内血流量、氧分压(pO2)和脑梗死染色,分析动物模型和OMB治疗结果的有效性。用动物行为学和脑梗死面积测量来评估脑功能的长期恢复。结果:脑卒中60 min、再灌注20 min、OMB治疗10 min后血流量百分比分别为45±3%、70±3%、86±2%,表现为超声灌注,相应的pO2水平分别为60±1%、76±2%、79±4%,表现为再氧合。治疗14天后,S/R小鼠脑梗死减少87±3%,肢体协调能力恢复。抑制NF-κB、HIF-1α、IL-1β和MMP-9的表达,增强eNOS、BDNF、Bcl2和IL-10的表达,提示其具有激活抗炎、抗凋亡反应和神经保护作用。我们的研究表明,OMB治疗结合了超声灌注和局部氧疗的有益作用,可以减少脑梗死,激活神经保护,防止S/R损伤。
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Oxygen-loaded microbubble-mediated sonoperfusion and oxygenation for neuroprotection after ischemic stroke reperfusion.

Background: Ischemic stroke-reperfusion (S/R) injury is a crucial issue in the protection of brain function after thrombolysis. The vasodilation induced by ultrasound (US)-stimulated microbubble cavitation has been applied to reduce S/R injury through sonoperfusion. The present study uses oxygen-loaded microbubbles (OMBs) with US stimulation to provide sonoperfusion and local oxygen therapy for the reduction of brain infarct size and neuroprotection after S/R.

Methods: The murine S/R model was established by photodynamic thrombosis and thrombolysis at the remote branch of the anterior cerebral artery. In vivo blood flow, partial oxygen pressure (pO2), and brain infarct staining were examined to analyze the validity of the animal model and OMB treatment results. The animal behaviors and measurement of the brain infarct area were used to evaluate long-term recovery of brain function.

Results: The percentage of blood flow was 45 ± 3%, 70 ± 3%, and 86 ± 2% after 60 min stroke, 20 min reperfusion, and 10 min OMB treatment, respectively, demonstrating sonoperfusion, and the corresponding pO2 level was 60 ± 1%, 76 ± 2%, and 79 ± 4%, showing reoxygenation. After 14 days of treatment, a 87 ± 3% reduction in brain infarction and recovery of limb coordination were observed in S/R mice. The expression of NF-κB, HIF-1α, IL-1β, and MMP-9 was inhibited and that of eNOS, BDNF, Bcl2, and IL-10 was enhanced, indicating activation of anti-inflammatory and anti-apoptosis responses and neuroprotection. Our study demonstrated that OMB treatment combines the beneficial effects of sonoperfusion and local oxygen therapy to reduce brain infarction and activate neuroprotection to prevent S/R injury.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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