José David Flores-Félix , Fernando Sánchez-Juanes , Juan Araujo , César Antonio Díaz-Alcántara , Encarna Velázquez , Fernando González-Andrés
{"title":"远明慢生根瘤菌的两个新的共生体,美洲和加勒比,厚脊慢生根瘤杆菌的共生体tropici和卡氏慢生根瘤虫的共生体cajani是多米尼加共和国豆科植物Cajanus cajan的微共生体。","authors":"José David Flores-Félix , Fernando Sánchez-Juanes , Juan Araujo , César Antonio Díaz-Alcántara , Encarna Velázquez , Fernando González-Andrés","doi":"10.1016/j.syapm.2023.126454","DOIUrl":null,"url":null,"abstract":"<div><p><span><em>Cajanus</em><em> cajan</em></span> L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop<em>.</em> Here we identified through MALDI-TOF MS several rhizobial strains nodulating <em>C. cajan</em> in two Dominican locations as <span><em>Bradyrhizobium</em><em> yuanmingense</em></span><span>. The phylogenetic analysis of </span><em>recA</em> and <em>glnII</em><span> housekeeping genes showed that these strains belong to a wide cluster together with the type strain of </span><em>B. yuanmingense</em> and other <em>C. cajan</em><span> nodulating strains previously isolated in Dominican Republic. The comparison of genomes from strains representative of different lineages within this cluster support the existence of several genospecies within </span><em>B. yuanmingense</em><span>, which is the major microsymbiont of </span><em>C. cajan</em> in Dominican Republic where it is also nodulated by <em>Bradyrhizobium cajani</em> and <em>Bradyrhizobium pachyrhizi</em>. The analysis of the symbiotic <em>nodC</em> gene showed that the <em>C. cajan</em> nodulating strains from the <em>B. yuanmingense</em> complex belong to two clusters with less than 90% similarity between them. The strains from these two clusters showed <em>nodC</em> gene similarity values lower than 90% with respect to the remaining <em>Bradyrhizobium</em> symbiovars and then they correspond to two new symbiovars for which we propose the names americaense and caribense. The results of the <em>nodC</em> gene analysis also showed that <em>C. cajan</em> is nodulated by the symbiovar tropici, which has been found by first time in this work within the species <em>Bradyrhizobium pachyrhizi</em>. These results confirmed the high promiscuity degree of <em>C. cajan</em>, which is also nodulated by the symbiovar cajani of <em>Bradyrhizobium cajani</em> in Dominican Republic.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 5","pages":"Article 126454"},"PeriodicalIF":3.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Two novel symbiovars of Bradyrhizobium yuanmingense, americaense and caribense, the symbiovar tropici of Bradyrhizobium pachyrhizi and the symbiovar cajani of Bradyrhizobium cajani are microsymbionts of the legume Cajanus cajan in Dominican Republic\",\"authors\":\"José David Flores-Félix , Fernando Sánchez-Juanes , Juan Araujo , César Antonio Díaz-Alcántara , Encarna Velázquez , Fernando González-Andrés\",\"doi\":\"10.1016/j.syapm.2023.126454\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><em>Cajanus</em><em> cajan</em></span> L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop<em>.</em> Here we identified through MALDI-TOF MS several rhizobial strains nodulating <em>C. cajan</em> in two Dominican locations as <span><em>Bradyrhizobium</em><em> yuanmingense</em></span><span>. The phylogenetic analysis of </span><em>recA</em> and <em>glnII</em><span> housekeeping genes showed that these strains belong to a wide cluster together with the type strain of </span><em>B. yuanmingense</em> and other <em>C. cajan</em><span> nodulating strains previously isolated in Dominican Republic. The comparison of genomes from strains representative of different lineages within this cluster support the existence of several genospecies within </span><em>B. yuanmingense</em><span>, which is the major microsymbiont of </span><em>C. cajan</em> in Dominican Republic where it is also nodulated by <em>Bradyrhizobium cajani</em> and <em>Bradyrhizobium pachyrhizi</em>. The analysis of the symbiotic <em>nodC</em> gene showed that the <em>C. cajan</em> nodulating strains from the <em>B. yuanmingense</em> complex belong to two clusters with less than 90% similarity between them. The strains from these two clusters showed <em>nodC</em> gene similarity values lower than 90% with respect to the remaining <em>Bradyrhizobium</em> symbiovars and then they correspond to two new symbiovars for which we propose the names americaense and caribense. The results of the <em>nodC</em> gene analysis also showed that <em>C. cajan</em> is nodulated by the symbiovar tropici, which has been found by first time in this work within the species <em>Bradyrhizobium pachyrhizi</em>. These results confirmed the high promiscuity degree of <em>C. cajan</em>, which is also nodulated by the symbiovar cajani of <em>Bradyrhizobium cajani</em> in Dominican Republic.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"46 5\",\"pages\":\"Article 126454\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000632\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000632","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Two novel symbiovars of Bradyrhizobium yuanmingense, americaense and caribense, the symbiovar tropici of Bradyrhizobium pachyrhizi and the symbiovar cajani of Bradyrhizobium cajani are microsymbionts of the legume Cajanus cajan in Dominican Republic
Cajanus cajan L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop. Here we identified through MALDI-TOF MS several rhizobial strains nodulating C. cajan in two Dominican locations as Bradyrhizobium yuanmingense. The phylogenetic analysis of recA and glnII housekeeping genes showed that these strains belong to a wide cluster together with the type strain of B. yuanmingense and other C. cajan nodulating strains previously isolated in Dominican Republic. The comparison of genomes from strains representative of different lineages within this cluster support the existence of several genospecies within B. yuanmingense, which is the major microsymbiont of C. cajan in Dominican Republic where it is also nodulated by Bradyrhizobium cajani and Bradyrhizobium pachyrhizi. The analysis of the symbiotic nodC gene showed that the C. cajan nodulating strains from the B. yuanmingense complex belong to two clusters with less than 90% similarity between them. The strains from these two clusters showed nodC gene similarity values lower than 90% with respect to the remaining Bradyrhizobium symbiovars and then they correspond to two new symbiovars for which we propose the names americaense and caribense. The results of the nodC gene analysis also showed that C. cajan is nodulated by the symbiovar tropici, which has been found by first time in this work within the species Bradyrhizobium pachyrhizi. These results confirmed the high promiscuity degree of C. cajan, which is also nodulated by the symbiovar cajani of Bradyrhizobium cajani in Dominican Republic.
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: