更好，更快，更便宜:冷冻电子显微镜的最新进展。

IF 12.1 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Annual review of biochemistry Pub Date : 2022-06-21 DOI:10.1146/annurev-biochem-032620-110705

Eugene Y D Chua, Joshua H Mendez, Micah Rapp, Serban L Ilca, Yong Zi Tan, Kashyap Maruthi, Huihui Kuang, Christina M Zimanyi, Anchi Cheng, Edward T Eng, Alex J Noble, Clinton S Potter, Bridget Carragher

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引用次数: 19

摘要

低温电子显微镜(cryo-EM)作为一种可视化生物物体的方法继续显着增长，这是由整个管道的进步所驱动的。单粒子分析和原位断层扫描技术的发展使得更多的结构能够以更高的分辨率、更快的速度进行成像和确定，更多的科学家也有了更好的途径。本综述重点介绍了低温电镜技术各个阶段的最新进展，并举例说明了这些技术如何用于研究现实世界的问题，包括在最近的COVID-19大流行期间针对SARS-CoV-2高峰的抗体开发。

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Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

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来源期刊

Annual review of biochemistry 生物-生化与分子生物学

CiteScore

33.90

自引率

0.00%

发文量

期刊介绍： The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.

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