K I Mosalev, I D Ivanov, S M Miroshnichenko, M V Tenditnik, N P Bgatova, E E Shults, V A Vavilin
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引用次数: 0
摘要
以呋喃香豆素(BABCF)为基础的间醛酸化合物的免疫调节活性研究了2-叠氮,9- n -甲基哌嗪甲基oreozelone)。以3月龄雄性C57BL/6小鼠(经细胞抑制剂环磷酰胺(cyclophosphamide, CP)处理)和完整个体为实验模型。在骨髓(IL-12、IL-10、IL-1β、TNF-α、TGF-β、M-CSF、GM-CSF)或腹膜细胞悬液(IL-12、IL-10;作为注射部位)。采用抗体流式细胞术检测静脉血单核细胞(MNCs)部分t淋巴细胞表面标志物(CD3、CD4和CD8)。进行组织学和形态计量学研究以评估CP和BABCF对胸腺的影响。BABCF导致GM-KSF基因的相对表达显著增加(约3倍)。BABCF引起cp处理小鼠腹腔细胞中IL-12表达的局部升高,恢复了血液中t淋巴细胞的相对含量,主要影响CD3 + CD4 +淋巴细胞。该物质降低了cp处理小鼠胸腺皮质和胸腺髓质的组织密度。因此,本研究结果表明,BABCF对免疫的细胞环节具有刺激作用,并且由于t淋巴细胞从免疫系统的中心器官迁移,促进血液中t淋巴细胞数量的维持。
The immunomodulatory activity of the betulonic acid based compound.
The immunomodulatory activity of a betulonic acid-based compound with furocoumarin (BABCF; 2-azido, 9-N-methylpiperazinomethyl oreozelone) has been investigated. Male C57BL/6 mice (aged 3 months) treated with the cytostatic agent cyclophosphamide (CP) and intact individuals served as experimental models. The expression of genes was studied in bone marrow (IL-12, IL-10, IL-1β, TNF-α, TGF-β, M-CSF, GM-CSF) or in the suspension of peritoneal cells (IL-12, IL-10; as the injection site). The surface markers of T-lymphocytes (CD3, CD4, and CD8) in fractions of venous blood mononuclear cells (MNCs) were determined by means of flow cytometry using antibodies. Histological and morphometric studies were performed to assess the impact of CP and BABCF on the thymus. BABCF caused a pronounced (about 3-fold) increase in relative expression of the GM-KSF gene. BABCF caused a local increase in the expression of IL-12 in the peritoneal cavity cells and restored the relative content of T-lymphocytes in the blood of CP-treated mice treated affecting mainly CD3⁺CD4⁺ lymphocytes. This substance reduced the tissue density of the thymic cortex and thymic medulla in CP-treated mice. Thus, results of this study suggest that BABCF exhibits a stimulating effect on the cellular link of immunity and promotes maintenance of the number of T-lymphocytes in the blood due to their migration from the central organs of the immune system.
Biomeditsinskaya khimiyaBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍:
The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).