质膜上类似SEC14的缩合物相变调节拟南芥根系的生长。

IF 7.8 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY PLoS Biology Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI:10.1371/journal.pbio.3002305
Chen Liu, Andriani Mentzelopoulou, Fotini Papagavriil, Prashanth Ramachandran, Artemis Perraki, Lucas Claus, Sebastian Barg, Peter Dörmann, Yvon Jaillais, Philipp Johnen, Eugenia Russinova, Electra Gizeli, Gabriel Schaaf, Panagiotis Nikolaou Moschou
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引用次数: 0

摘要

蛋白质的功能可以通过其材料性质的相变来调节,材料性质可以从液态到固态;然而,驱动这些转变的机制以及它们对生理学是否重要仍然未知。在模式植物拟南芥中,我们发现质膜结合的脂质结合蛋白SEC14-like的相变增强了发育的稳健性。利用成像、遗传学和体外重建实验,我们发现SEC14-like在根干细胞中经历液相分离。在干细胞生态位之外,SEC14样与胱天蛋白酶样蛋白酶分离酶结合,并在独特的极性质膜界面上保守微管马达。在这些界面中,SEC14-like通过分离酶进行处理,从而促进其从液体到固体的转变。这种转变对根系发育很重要,因为表达分离酶和相关微管马达的不可清除的SEC14样变体或突变体的系表现出相似的发育表型。此外,加工和固化但不是液体形式的SEC14-like与生长素流出载体PINFORMED2相互作用并调节其极性。这项工作表明,稳健的发展可能涉及在独特的质膜界面上通过蛋白水解介导的液体到固体的转变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis.

Protein function can be modulated by phase transitions in their material properties, which can range from liquid- to solid-like; yet, the mechanisms that drive these transitions and whether they are important for physiology are still unknown. In the model plant Arabidopsis, we show that developmental robustness is reinforced by phase transitions of the plasma membrane-bound lipid-binding protein SEC14-like. Using imaging, genetics, and in vitro reconstitution experiments, we show that SEC14-like undergoes liquid-like phase separation in the root stem cells. Outside the stem cell niche, SEC14-like associates with the caspase-like protease separase and conserved microtubule motors at unique polar plasma membrane interfaces. In these interfaces, SEC14-like undergoes processing by separase, which promotes its liquid-to-solid transition. This transition is important for root development, as lines expressing an uncleavable SEC14-like variant or mutants of separase and associated microtubule motors show similar developmental phenotypes. Furthermore, the processed and solidified but not the liquid form of SEC14-like interacts with and regulates the polarity of the auxin efflux carrier PINFORMED2. This work demonstrates that robust development can involve liquid-to-solid transitions mediated by proteolysis at unique plasma membrane interfaces.

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来源期刊
PLoS Biology
PLoS Biology 生物-生化与分子生物学
CiteScore
14.40
自引率
2.00%
发文量
359
审稿时长
3 months
期刊介绍: PLOS Biology is an open-access, peer-reviewed general biology journal published by PLOS, a nonprofit organization of scientists and physicians dedicated to making the world's scientific and medical literature freely accessible. The journal publishes new articles online weekly, with issues compiled and published monthly. ISSN Numbers: eISSN: 1545-7885 ISSN: 1544-9173
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