氯喹通过抑制自噬使食管癌EC109细胞对紫杉醇增敏。

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2023046722
Zichun Yuan, Jiajing Cai, Qin Du, Qiang Ma, Lei Xu, Yan Cai, Xiaowu Zhong, Xiaolan Guo
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引用次数: 0

摘要

作为一种自噬抑制剂,氯喹(chloroquine, CQ)对多种类型的肿瘤具有抗肿瘤作用,紫杉醇(paclitaxel, PTX)广泛应用于食管癌患者的治疗,但由于紫杉醇具有细胞保护性自噬作用,化疗耐药一直是制约其应用的主要障碍。因此,本研究旨在探讨CQ是否能提高PTX对食管癌细胞系EC109的抗肿瘤作用,并探讨其可能的分子机制。通过MTT、划痕试验、transwell和软琼脂试验证实了PTX对EC109的抑制作用。Western Blot检测Akt/mTOR通路的关键蛋白,以及自噬标志物LC3和p62。此外,将GFP-LC3质粒转染到EC109细胞中,监测CQ和PTX处理后的自噬体。最后,我们观察了shRNA敲低mTOR后EC109的增殖和集落形成能力的变化。我们证实PTX可以抑制EC109的增殖、迁移和集落形成能力(均P < 0.05), CQ可以通过Akt/mTOR途径抑制自噬,从而增强PTX的抑制作用。抑制Akt/mTOR通路启动自噬,增强EC109对CQ和PTX的敏感性。综上所述,我们建议CQ可以作为PTX治疗食管癌的潜在化学增敏剂。
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Chloroquine Sensitizes Esophageal Carcinoma EC109 Cells to Paclitaxel by Inhibiting Autophagy.

As an autophagy inhibitor, chloroquine (CQ) showed anti-tumor effect on several types of cancer and paclitaxel (PTX) is widely used in the treatment of esophageal carcinoma patients, but chemoresistance remains a major hurdle for PTX application due to the cytoprotective autophagy. Therefore, the aim of this study was to investigate whether CQ could elevate the anti-tumor effect of PTX on esophageal carcinoma cell line EC109 and explore the potential molecular mechanisms. We confirmed the suppressive effect of PTX on EC109 by MTT, scratch test, transwell and soft agar assay. And, we detected the key proteins in Akt/mTOR pathway, as well as the autophagy marker LC3 and p62 through Western Blot. In addition, GFP-LC3 plasmid was transfected into EC109 cells to monitor the autophagosome after CQ and PTX treatment. Ultimately, we observed the alterations in the proliferation and colony formation abilities of EC109 after knocking down mTOR by shRNA. We confirmed PTX could suppress the proliferation, migration and colony formation (all P < 0.05) abilities of EC109, and CQ could sensitize the inhibition effect of PTX by inhibiting autophagy through Akt/mTOR pathway. Furthermore, inhibiting Akt/mTOR pathway initiated autophagy and enhanced the sensitivity of EC109 to CQ and PTX. In summary, we suggest CQ could be used as a potential chemosensitizer for PTX in esophageal carcinoma treatment.

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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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