荧光标记吡唑衍生物在拟南芥幼苗中诱导三重反应的合成。

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2022-11-20 DOI:10.1584/jpestics.D22-006
Keimei Oh, Kai Jiang
{"title":"荧光标记吡唑衍生物在拟南芥幼苗中诱导三重反应的合成。","authors":"Keimei Oh,&nbsp;Kai Jiang","doi":"10.1584/jpestics.D22-006","DOIUrl":null,"url":null,"abstract":"<p><p>A fluorescent labeled pyrazole derivative with a dansyl moiety (<b>EH-DF</b>) was synthesized. Design of <b>EH-DF</b> was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (<b>EH-1</b>). At a concentration of 30 µM, <b>EH-DF</b> displayed biological activity on inducing a triple response in <i>Arabidopsis</i> seedlings. Compared with the non-chemical treated control, the hypocotyl length of <b>EH-DF</b>-treated <i>Arabidopsis</i> seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of <i>Arabidopsis</i> seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of <b>EH-DF</b> were 350 and 535 nm, respectively. Data obtained <i>via</i> fluorescent microscope analysis indicated that intensive fluorescent signals of <b>EH-DF</b> were observed in the shoot of <i>Arabidopsis</i> seedlings.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/cf/jps-47-4-D22-006.PMC9716048.pdf","citationCount":"0","resultStr":"{\"title\":\"Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in <i>Arabidopsis</i> seedlings.\",\"authors\":\"Keimei Oh,&nbsp;Kai Jiang\",\"doi\":\"10.1584/jpestics.D22-006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A fluorescent labeled pyrazole derivative with a dansyl moiety (<b>EH-DF</b>) was synthesized. Design of <b>EH-DF</b> was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (<b>EH-1</b>). At a concentration of 30 µM, <b>EH-DF</b> displayed biological activity on inducing a triple response in <i>Arabidopsis</i> seedlings. Compared with the non-chemical treated control, the hypocotyl length of <b>EH-DF</b>-treated <i>Arabidopsis</i> seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of <i>Arabidopsis</i> seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of <b>EH-DF</b> were 350 and 535 nm, respectively. Data obtained <i>via</i> fluorescent microscope analysis indicated that intensive fluorescent signals of <b>EH-DF</b> were observed in the shoot of <i>Arabidopsis</i> seedlings.</p>\",\"PeriodicalId\":16712,\"journal\":{\"name\":\"Journal of Pesticide Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2022-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/cf/jps-47-4-D22-006.PMC9716048.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pesticide Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1584/jpestics.D22-006\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.D22-006","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

合成了一种荧光标记的丹酰吡唑衍生物。EH-DF的设计是用丹酰部分代替母体化合物EH-1的萘部分。在浓度为30µM时,EH-DF在拟南芥幼苗中表现出诱导三重反应的生物活性。与未经化学处理的对照相比,eh - df处理的拟南芥幼苗下胚轴长度从约9.2±0.7 mm缩短至2.4±0.2 mm。根长从1.7±0.1 mm减少到1.0±0.1 mm,拟南芥幼苗钩曲率从60±16度增加到245±35度。EH-DF的最大激发波长为350 nm,最大发射波长为535 nm。荧光显微镜分析数据表明,EH-DF在拟南芥幼苗的茎部中观察到强烈的荧光信号。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in Arabidopsis seedlings.

A fluorescent labeled pyrazole derivative with a dansyl moiety (EH-DF) was synthesized. Design of EH-DF was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (EH-1). At a concentration of 30 µM, EH-DF displayed biological activity on inducing a triple response in Arabidopsis seedlings. Compared with the non-chemical treated control, the hypocotyl length of EH-DF-treated Arabidopsis seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of Arabidopsis seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of EH-DF were 350 and 535 nm, respectively. Data obtained via fluorescent microscope analysis indicated that intensive fluorescent signals of EH-DF were observed in the shoot of Arabidopsis seedlings.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
期刊最新文献
Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates. Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1