{"title":"荧光标记吡唑衍生物在拟南芥幼苗中诱导三重反应的合成。","authors":"Keimei Oh, Kai Jiang","doi":"10.1584/jpestics.D22-006","DOIUrl":null,"url":null,"abstract":"<p><p>A fluorescent labeled pyrazole derivative with a dansyl moiety (<b>EH-DF</b>) was synthesized. Design of <b>EH-DF</b> was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (<b>EH-1</b>). At a concentration of 30 µM, <b>EH-DF</b> displayed biological activity on inducing a triple response in <i>Arabidopsis</i> seedlings. Compared with the non-chemical treated control, the hypocotyl length of <b>EH-DF</b>-treated <i>Arabidopsis</i> seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of <i>Arabidopsis</i> seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of <b>EH-DF</b> were 350 and 535 nm, respectively. Data obtained <i>via</i> fluorescent microscope analysis indicated that intensive fluorescent signals of <b>EH-DF</b> were observed in the shoot of <i>Arabidopsis</i> seedlings.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/cf/jps-47-4-D22-006.PMC9716048.pdf","citationCount":"0","resultStr":"{\"title\":\"Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in <i>Arabidopsis</i> seedlings.\",\"authors\":\"Keimei Oh, Kai Jiang\",\"doi\":\"10.1584/jpestics.D22-006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A fluorescent labeled pyrazole derivative with a dansyl moiety (<b>EH-DF</b>) was synthesized. Design of <b>EH-DF</b> was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (<b>EH-1</b>). At a concentration of 30 µM, <b>EH-DF</b> displayed biological activity on inducing a triple response in <i>Arabidopsis</i> seedlings. Compared with the non-chemical treated control, the hypocotyl length of <b>EH-DF</b>-treated <i>Arabidopsis</i> seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of <i>Arabidopsis</i> seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of <b>EH-DF</b> were 350 and 535 nm, respectively. Data obtained <i>via</i> fluorescent microscope analysis indicated that intensive fluorescent signals of <b>EH-DF</b> were observed in the shoot of <i>Arabidopsis</i> seedlings.</p>\",\"PeriodicalId\":16712,\"journal\":{\"name\":\"Journal of Pesticide Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2022-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/cf/jps-47-4-D22-006.PMC9716048.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pesticide Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1584/jpestics.D22-006\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.D22-006","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in Arabidopsis seedlings.
A fluorescent labeled pyrazole derivative with a dansyl moiety (EH-DF) was synthesized. Design of EH-DF was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (EH-1). At a concentration of 30 µM, EH-DF displayed biological activity on inducing a triple response in Arabidopsis seedlings. Compared with the non-chemical treated control, the hypocotyl length of EH-DF-treated Arabidopsis seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of Arabidopsis seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of EH-DF were 350 and 535 nm, respectively. Data obtained via fluorescent microscope analysis indicated that intensive fluorescent signals of EH-DF were observed in the shoot of Arabidopsis seedlings.
期刊介绍:
The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.