Şükran Keskin Gözmen , Erkin Serdaroğlu , Nida Dinçel , Pınar Erturgut , Cemaliye Başaran , Fatma Devrim , Betül Pehlivan Zorlu , Özlem Dur , Orhan Deniz Kara , Ebru Bekiroğlu Yilmaz
{"title":"全自动全血计数器、尿液分析仪和尿试纸试验结果与肿瘤细胞计数室计数在儿童透析相关性腹膜炎诊断中的相关性","authors":"Şükran Keskin Gözmen , Erkin Serdaroğlu , Nida Dinçel , Pınar Erturgut , Cemaliye Başaran , Fatma Devrim , Betül Pehlivan Zorlu , Özlem Dur , Orhan Deniz Kara , Ebru Bekiroğlu Yilmaz","doi":"10.1016/j.nephro.2022.10.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>Peritoneal dialysis<span><span> is the treatment<span> of choice for end-stage renal disease. Peritoneal dialysis related peritonitis is of great importance for patient and technical survival. The aim of our study was to evaluate the accuracy and the correlation between the three methods (complete blood count, </span></span>urinalysis device, urine dipstick test) and with the reference manual method (Thoma Cell Counter Chamber).</span></p></div><div><h3>Materials and methods</h3><p>We retrospectively analyzed 167 peritoneal fluid samples taken from 25 patients receiving peritoneal dialysis treatment. Leukocyte counts were evaluated with Thoma Cell Counter Chamber, complete blood count, urinalysis device and urine dipstick test.</p></div><div><h3>Results</h3><p>There was a significant positive correlation between Thoma Cell Counter Chamber and complete blood count results (Spearman's rho<!--> <!-->=<!--> <!-->0.70), between Thoma Cell Counter Chamber and urinalysis device (Spearman's rho<!--> <!-->=<!--> <!-->0.73), and between Thoma Cell Counter Chamber and urine dipstick test (Spearman's rho<!--> <!-->=<!--> <!-->0.71). Area under curve for complete blood count, urinalysis device and urine dipstick test were 0.93, 0.94 and 0.89 respectively, indicating good accuracy. Sensitivity and specificity were 89.7% and 86.7% in the complete blood count analysis (associated criterion: 130 cells/mm<sup>3</sup>). Sensitivity and specificity were 89.7% and 86.7% in the urinalysis device (associated criterion: 10 cells/HPF). Sensitivity and specificity were 79.6% and 91.4% when in the urine dipstick test analysis (associated criterion: +<!--> <!-->1 positivity). The Bland-Altman plot showed good agreement.</p></div><div><h3>Conclusion</h3><p>Automatic complete blood count and urinalysis devices have good correlation and agreement with manual method in the diagnosis of peritonitis in the pediatric age group. Urine dipstick test in the home setting can be useful for screening patients with suspected peritonitis.</p></div>","PeriodicalId":51140,"journal":{"name":"Nephrologie & Therapeutique","volume":"18 7","pages":"Pages 611-615"},"PeriodicalIF":0.7000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Automated complete blood counter, urine analyzer and urine dipstick test results are correlated with thoma cell counting chamber counts in the diagnosis of dialysis related peritonitis in children\",\"authors\":\"Şükran Keskin Gözmen , Erkin Serdaroğlu , Nida Dinçel , Pınar Erturgut , Cemaliye Başaran , Fatma Devrim , Betül Pehlivan Zorlu , Özlem Dur , Orhan Deniz Kara , Ebru Bekiroğlu Yilmaz\",\"doi\":\"10.1016/j.nephro.2022.10.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>Peritoneal dialysis<span><span> is the treatment<span> of choice for end-stage renal disease. Peritoneal dialysis related peritonitis is of great importance for patient and technical survival. The aim of our study was to evaluate the accuracy and the correlation between the three methods (complete blood count, </span></span>urinalysis device, urine dipstick test) and with the reference manual method (Thoma Cell Counter Chamber).</span></p></div><div><h3>Materials and methods</h3><p>We retrospectively analyzed 167 peritoneal fluid samples taken from 25 patients receiving peritoneal dialysis treatment. Leukocyte counts were evaluated with Thoma Cell Counter Chamber, complete blood count, urinalysis device and urine dipstick test.</p></div><div><h3>Results</h3><p>There was a significant positive correlation between Thoma Cell Counter Chamber and complete blood count results (Spearman's rho<!--> <!-->=<!--> <!-->0.70), between Thoma Cell Counter Chamber and urinalysis device (Spearman's rho<!--> <!-->=<!--> <!-->0.73), and between Thoma Cell Counter Chamber and urine dipstick test (Spearman's rho<!--> <!-->=<!--> <!-->0.71). Area under curve for complete blood count, urinalysis device and urine dipstick test were 0.93, 0.94 and 0.89 respectively, indicating good accuracy. Sensitivity and specificity were 89.7% and 86.7% in the complete blood count analysis (associated criterion: 130 cells/mm<sup>3</sup>). Sensitivity and specificity were 89.7% and 86.7% in the urinalysis device (associated criterion: 10 cells/HPF). Sensitivity and specificity were 79.6% and 91.4% when in the urine dipstick test analysis (associated criterion: +<!--> <!-->1 positivity). The Bland-Altman plot showed good agreement.</p></div><div><h3>Conclusion</h3><p>Automatic complete blood count and urinalysis devices have good correlation and agreement with manual method in the diagnosis of peritonitis in the pediatric age group. Urine dipstick test in the home setting can be useful for screening patients with suspected peritonitis.</p></div>\",\"PeriodicalId\":51140,\"journal\":{\"name\":\"Nephrologie & Therapeutique\",\"volume\":\"18 7\",\"pages\":\"Pages 611-615\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nephrologie & Therapeutique\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1769725522006137\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nephrologie & Therapeutique","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1769725522006137","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
Automated complete blood counter, urine analyzer and urine dipstick test results are correlated with thoma cell counting chamber counts in the diagnosis of dialysis related peritonitis in children
Introduction
Peritoneal dialysis is the treatment of choice for end-stage renal disease. Peritoneal dialysis related peritonitis is of great importance for patient and technical survival. The aim of our study was to evaluate the accuracy and the correlation between the three methods (complete blood count, urinalysis device, urine dipstick test) and with the reference manual method (Thoma Cell Counter Chamber).
Materials and methods
We retrospectively analyzed 167 peritoneal fluid samples taken from 25 patients receiving peritoneal dialysis treatment. Leukocyte counts were evaluated with Thoma Cell Counter Chamber, complete blood count, urinalysis device and urine dipstick test.
Results
There was a significant positive correlation between Thoma Cell Counter Chamber and complete blood count results (Spearman's rho = 0.70), between Thoma Cell Counter Chamber and urinalysis device (Spearman's rho = 0.73), and between Thoma Cell Counter Chamber and urine dipstick test (Spearman's rho = 0.71). Area under curve for complete blood count, urinalysis device and urine dipstick test were 0.93, 0.94 and 0.89 respectively, indicating good accuracy. Sensitivity and specificity were 89.7% and 86.7% in the complete blood count analysis (associated criterion: 130 cells/mm3). Sensitivity and specificity were 89.7% and 86.7% in the urinalysis device (associated criterion: 10 cells/HPF). Sensitivity and specificity were 79.6% and 91.4% when in the urine dipstick test analysis (associated criterion: + 1 positivity). The Bland-Altman plot showed good agreement.
Conclusion
Automatic complete blood count and urinalysis devices have good correlation and agreement with manual method in the diagnosis of peritonitis in the pediatric age group. Urine dipstick test in the home setting can be useful for screening patients with suspected peritonitis.
期刊介绍：
Organe d''expression de la Société de Néphrologie, de la Société Francophone de Dialyse et de la Société de Néphrologie Pédiatrique, Néphrologie et Thérapeutique a pour vocation de publier des textes en français dans le domaine de la Néphrologie, qu''il s''agisse d''actualisation des connaissances, de recommandations de bonne pratique clinique, de publications originales, ou d''informations sur la vie des trois sociétés fondatrices. La variété des thèmes abordés reflète la richesse de la Néphrologie, qu''il s''agisse d''aspects fondamentaux issus de la physiologie, de l''immunologie, de l''anatomo-pathologie, ou de la génétique, ou de sujets de néphrologie clinique, notamment ceux en rapport avec les thérapeutiques néphrologiques, transplantation, hémodialyse et dialyse péritonéale.
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