基于连续小波变换和多元校准模型的简单紫外分光光度法,用于同时分析生育保健品中的三种水溶性维生素。

IF 1.7 4区 农林科学 Q3 CHEMISTRY, ANALYTICAL Journal of AOAC International Pub Date : 2024-01-04 DOI:10.1093/jaoacint/qsad093
Mahla Lohrdy, Mahmoud Reza Sohrabi, Mehran Davallo
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引用次数: 0

摘要

背景:由于药物成分中存在重叠光谱,传统的分光光度法很难同时进行测定。化学计量学和紫外可见分光光度法的发展有助于解决这一问题:本研究采用连续小波变换(CWT)和偏最小二乘法(PLS)技术,在不使用耗时的提取过程和有机溶剂的情况下,引入并验证了一种快速、简便、精确、低成本且环保的分光光度法,用于同时分析男性和女性生育保健品中的维生素 B6、维生素 B12 和维生素 C:方法:在 CWT 方法中,应用零交叉技术来获得绘制各成分校准曲线的最佳点。通过分析几种不同浓度的混合物,对这两种方法的有效性进行了评估。此外,还对商用胶囊的效率进行了调查:对维生素 B6、B12 和 C 分别采用了小波系列,包括 230 纳米波长的 Symlet (sym2)、378 纳米波长的 Biorthogonal (bior1.3) 和 261 纳米波长的 Daubechies (db2)。结果发现,维生素 B6、维生素 B12 和维生素 C 的线性范围分别为 8-20、8-20 和 10-25 μg/mL,测定系数 (R2) 分别为 0.9982、0.9978 和 0.9701。检测限(LOD)较低(结论):通过方差分析(ANOVA)测试,将采用建议的技术从商品胶囊中获得的结果与高效液相色谱法(HPLC)获得的结果进行了比较。结果表明,二者没有明显差异,一致性良好。根据上述所有情况,所提出的方法可以在质量控制实验室中取代费时费力的高效液相色谱法:提出了绿色分光光度法耦合化学计量学方法。亮点:提出了绿色分光光度法耦合化学计量学方法,利用这些方法同时测定了生育补充剂中的三种水溶性维生素。所提方法具有快速、简便、低成本和准确等优点。使用高效液相色谱技术作为参考方法,与化学计量学方法进行比较。
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A Simple UV Spectrophotometric Method Based on Continuous Wavelet Transform and Multivariate Calibration Model for the Concurrent Analysis of Three Water-Soluble Vitamins in Fertility Supplements.

Background: Owing to the presence of overlapping spectra in pharmaceutical components, classical spectrophotometry is hard for concurrent determination. The advance of chemometrics along with UV-Vis spectrophotometry has contributed to solving this problem.

Objective: In this study, a fast, easy, precise, accurate, low-cost, and eco-friendly spectrophotometric technique was introduced and validated for the simultaneous analysis of vitamin B6, vitamin B12, and vitamin C in fertility supplements for men and women using continuous wavelet transform (CWT) and partial least squares (PLS) techniques without using time-consuming extraction process and organic solvents.

Method: In the CWT method, the zero-crossing technique was applied to obtain the optimum points for plotting calibration curves for each component. The validation of both methods was evaluated by analyzing several mixtures with different concentrations. The efficiency of the proposed methods was also surveyed on commercial capsules.

Results: Wavelet families, including Symlet (sym2) at 230, Biorthogonal (bior1.3) at 378 nm, and Daubechies (db2) at 261, were considered for vitamins B6, B12, and C, respectively. The linear range was found to be 8-20, 8-20, and 10-25 μg/mL with the coefficient of determination (R2) equal to 0.9982, 0.9978, and 0.9701 for B6, B12, and C, respectively. Low limit of detection (LOD) (<0.09 μg/mL) and limit of quantification (LOQ) <0.9 μg/mL were achieved. The mean recovery values in synthetic mixtures were from 98.38 to 98.89% and from 99.83 to 99.99%, where root-mean-square error (RMSE) of not more than 0.4 and 0.05 using the CWT and PLS methods, respectively.

Conclusions: The obtained results from the commercial capsules, applying the suggested techniques, were compared to those yielded by the high-performance liquid chromatography (HPLC) method using the analysis of variance (ANOVA) test. According to the results, there are no significant differences, and they were in good agreement. According to all the mentioned cases, the proposed approaches can replace the time-consuming and costly HPLC method in quality control laboratories.

Highlights: Green spectrophotometry coupling chemometrics methods were proposed. Simultaneous determination of three water-soluble vitamins in fertility supplements was done using these approaches. Rapidity, simplicity, low cost, and accuracy are the benefits of the proposed methods. A HPLC technique was used as a reference method to compare with the chemometrics methods.

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来源期刊
Journal of AOAC International
Journal of AOAC International 医学-分析化学
CiteScore
3.10
自引率
12.50%
发文量
144
审稿时长
2.7 months
期刊介绍: The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.
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