Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi
{"title":"姜黄素通过rek /基质金属蛋白酶轴的表观遗传重编程对A-549肺癌细胞的抗迁移作用","authors":"Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi","doi":"10.1515/hmbci-2021-0100","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, <i>RECK</i>/<i>MMPs</i> axis as a probable regulator of cancer cell migration was assessed.</p><p><strong>Methods: </strong>In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of <i>RECK</i> gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including <i>CASP3</i>, <i>CASP8</i>, <i>CASP9</i>, <i>BAX</i>, <i>BCL2</i>, <i>MMP9</i>, <i>MMP2</i>, and <i>RECK</i>) were investigated by quantitative Real-Time PCR.</p><p><strong>Results: </strong>The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the <i>RECK</i> gene promoter after curcumin treatment.</p><p><strong>Conclusions: </strong>Curcumin inhibited lung cancer cells through various molecular pathways. <i>RECK</i>/<i>MMPs</i> axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.</p>","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of <i>RECK/</i> matrix metalloproteinase axis.\",\"authors\":\"Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi\",\"doi\":\"10.1515/hmbci-2021-0100\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, <i>RECK</i>/<i>MMPs</i> axis as a probable regulator of cancer cell migration was assessed.</p><p><strong>Methods: </strong>In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of <i>RECK</i> gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including <i>CASP3</i>, <i>CASP8</i>, <i>CASP9</i>, <i>BAX</i>, <i>BCL2</i>, <i>MMP9</i>, <i>MMP2</i>, and <i>RECK</i>) were investigated by quantitative Real-Time PCR.</p><p><strong>Results: </strong>The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the <i>RECK</i> gene promoter after curcumin treatment.</p><p><strong>Conclusions: </strong>Curcumin inhibited lung cancer cells through various molecular pathways. <i>RECK</i>/<i>MMPs</i> axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.</p>\",\"PeriodicalId\":13224,\"journal\":{\"name\":\"Hormone Molecular Biology and Clinical Investigation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hormone Molecular Biology and Clinical Investigation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/hmbci-2021-0100\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hormone Molecular Biology and Clinical Investigation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/hmbci-2021-0100","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis.
Objectives: The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, RECK/MMPs axis as a probable regulator of cancer cell migration was assessed.
Methods: In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of RECK gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including CASP3, CASP8, CASP9, BAX, BCL2, MMP9, MMP2, and RECK) were investigated by quantitative Real-Time PCR.
Results: The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the RECK gene promoter after curcumin treatment.
Conclusions: Curcumin inhibited lung cancer cells through various molecular pathways. RECK/MMPs axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.
期刊介绍：
Hormone Molecular Biology and Clinical Investigation (HMBCI) is dedicated to the provision of basic data on molecular aspects of hormones in physiology and pathophysiology. The journal covers the treatment of major diseases, such as endocrine cancers (breast, prostate, endometrium, ovary), renal and lymphoid carcinoma, hypertension, cardiovascular systems, osteoporosis, hormone deficiency in menopause and andropause, obesity, diabetes, brain and related diseases, metabolic syndrome, sexual dysfunction, fetal and pregnancy diseases, as well as the treatment of dysfunctions and deficiencies. HMBCI covers new data on the different steps and factors involved in the mechanism of hormone action. It will equally examine the relation of hormones with the immune system and its environment, as well as new developments in hormone measurements. HMBCI is a blind peer reviewed journal and publishes in English: Original articles, Reviews, Mini Reviews, Short Communications, Case Reports, Letters to the Editor and Opinion papers. Ahead-of-print publishing ensures faster processing of fully proof-read, DOI-citable articles.
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