Daiki Hashimoto, Kota Fujimoto, Sang Woon Kim, Yong Seung Lee, Masanori Nakata, Kentaro Suzuki, Yoshitaka Wada, Shinichi Asamura, Gen Yamada
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引用次数: 0
摘要
背景：含有窦道的海绵体（CC）在勃起过程中发挥着重要作用。勃起系统的病理变化分析是通过最新的实验系统进行研究的。方法：对小鼠 CC 的切片图像进行三维重建。通过基因表达研究发现，老龄小鼠CC的异位软骨生成显示了Sox9的显著表达。介绍了利用间充质衍生原代细胞和组织外植体的各种实验策略：主要发现：提出了 Sox9 在软骨形成中的可能作用及其受多种信号的调控。通过对外生殖器（ExG）衍生细胞和外植体培养物的各种分析，显示了生殖器间充质的独特性。这些策略还被应用于分析勃起收缩/松弛对多种信号和衰老过程的反应：勃起功能障碍（ED）是现代老年社会的重要课题之一。有必要开展更全面的研究，结合多种细胞培养策略揭示勃起系统的本质。
Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids.
Background: The corpus cavernosum (CC) containing sinusoids plays fundamental roles for erection. Analysis of pathological changes in the erectile system is studied by recent experimental systems. Various in vitro models utilizing genital mesenchymal-derived cells and explant culture systems are summarized.
Methods: 3D reconstruction of section images of murine CC was created. Ectopic chondrogenesis in aged mouse CC was shown by a gene expression study revealing the prominent expression of Sox9. Various experimental strategies utilizing mesenchyme-derived primary cells and tissue explants are introduced.
Main findings: Possible roles of Sox9 in chondrogenesis and its regulation by several signals are suggested. The unique character of genital mesenchyme is shown by various analyses of external genitalia (ExG) derived cells and explant cultures. Such strategies are also applied to the analysis of erectile contraction/relaxation responses to many signals and aging process.
Conclusion: Erectile dysfunction (ED) is one of the essential topics for the modern aged society. More comprehensive studies are necessary to reveal the nature of the erectile system by combining multiple cell culture strategies.
期刊介绍：
Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.
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