敲除多药外排泵MexXY-OprM导致铜绿假单胞菌对抗菌肽的敏感性增加

IF 1.9 4区医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2023-07-09 DOI:10.1111/1348-0421.13089

Anke Neidig, Nikola Strempel, Nadine Bianca Waeber, Waleska Stephanie da Cruz Nizer, Joerg Overhage

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摘要

耐药-结瘤-细胞分裂家族的多药外排系统在铜绿假单胞菌对多种抗生素的耐药中起着至关重要的作用。在这里，我们研究了临床相关的外排泵MexAB−OprM、MexCD−OprJ和MexXY−OprM在对不同阳离子抗菌肽(amp)的耐药性中的作用。我们的结果表明，外排泵MexXY-OprM的敲除使对某些amp的易感性增加了2至8倍。我们的数据表明，MexXY-OprM在铜绿假单胞菌对某些amp的耐药性中发挥了作用，这应该在未来开发新的高活性抗菌肽以对抗多药耐药感染时加以考虑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Knock-out of multidrug efflux pump MexXY-OprM results in increased susceptibility to antimicrobial peptides in Pseudomonas aeruginosa

Multidrug efflux systems of the resistance-nodulation-cell division family play a crucial role in resistance of Pseudomonas aeruginosa to a large variety of antibiotics. Here, we investigated the role of clinically relevant efflux pumps MexAB⁻OprM, MexCD⁻OprJ, and MexXY⁻OprM in resistance against different cationic antimicrobial peptides (AMPs). Our results indicate that a knock-out in efflux pump MexXY-OprM increased susceptibility to some AMPs by two- to eightfold. Our data suggest a contribution of MexXY-OprM in resistance to certain AMPs in P. aeruginosa, which should be considered in the future development of new and highly active antimicrobial peptides to fight multidrug resistant infections.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.