{"title":"印度孔雀(Pavo critatus)楔状前胸的形态学、分子生物学和病理学研究。","authors":"Asok Kumar Mariappan, Megha Sharma, Karikalan Mathesh, Vivek Srinivas Mouttou, Hiraram, Abhijeet Pawde, Dhama Kuldeep, G Saikumar","doi":"10.1080/03079457.2023.2251917","DOIUrl":null,"url":null,"abstract":"<p><p>This report provides the first record of <i>Prosthogonimus cuneatus</i> infection in Indian peafowl. Chickens, turkeys, geese, ducks, and other pet birds are recognized as direct hosts of <i>Prosthogonimus</i> species; however, <i>P. cuneatus</i> has not been reported to infect peafowl globally. Here, we identify the trematode present in the bursa of the peafowl by both morphological and molecular methods, in addition to the changes in the bursal tissue induced by the parasite, using histopathology. After a necropsy examination, the trematodes were found in the bursa of Fabricius in three peafowl. Morphological and molecular approaches based on taxonomic characteristics and the sequencing of the trematode-specific internal transcribed spacer (ITS) gene, respectively, were employed for trematode identification. The consensus sequences were compared to <i>P. cuneatus</i> reference sequences from GenBank. In order to assess the pathology caused by the parasite, a histological study of the bursa was also performed. Trematodes were confirmed as <i>P. cuneatus</i> based on morphology and DNA sequencing. Further, histopathological evaluation revealed mild lymphoid depletion of the bursal follicles in both the cortex and medulla with associated thinning of the bursal follicular lining epithelium. Indian peafowl can act as a natural host of <i>P. cuneatus</i>. This study provides a detailed pathological and molecular analysis of <i>P. cuneatus</i> affecting Indian peafowl.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Morphological, molecular, and pathological studies on <i>Prosthogonimus cuneatus</i> in Indian peacocks (<i>Pavo cristatus</i>).\",\"authors\":\"Asok Kumar Mariappan, Megha Sharma, Karikalan Mathesh, Vivek Srinivas Mouttou, Hiraram, Abhijeet Pawde, Dhama Kuldeep, G Saikumar\",\"doi\":\"10.1080/03079457.2023.2251917\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This report provides the first record of <i>Prosthogonimus cuneatus</i> infection in Indian peafowl. Chickens, turkeys, geese, ducks, and other pet birds are recognized as direct hosts of <i>Prosthogonimus</i> species; however, <i>P. cuneatus</i> has not been reported to infect peafowl globally. Here, we identify the trematode present in the bursa of the peafowl by both morphological and molecular methods, in addition to the changes in the bursal tissue induced by the parasite, using histopathology. After a necropsy examination, the trematodes were found in the bursa of Fabricius in three peafowl. Morphological and molecular approaches based on taxonomic characteristics and the sequencing of the trematode-specific internal transcribed spacer (ITS) gene, respectively, were employed for trematode identification. The consensus sequences were compared to <i>P. cuneatus</i> reference sequences from GenBank. In order to assess the pathology caused by the parasite, a histological study of the bursa was also performed. Trematodes were confirmed as <i>P. cuneatus</i> based on morphology and DNA sequencing. Further, histopathological evaluation revealed mild lymphoid depletion of the bursal follicles in both the cortex and medulla with associated thinning of the bursal follicular lining epithelium. Indian peafowl can act as a natural host of <i>P. cuneatus</i>. This study provides a detailed pathological and molecular analysis of <i>P. cuneatus</i> affecting Indian peafowl.</p>\",\"PeriodicalId\":8788,\"journal\":{\"name\":\"Avian Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Avian Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/03079457.2023.2251917\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/9/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avian Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/03079457.2023.2251917","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/9/5 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Morphological, molecular, and pathological studies on Prosthogonimus cuneatus in Indian peacocks (Pavo cristatus).
This report provides the first record of Prosthogonimus cuneatus infection in Indian peafowl. Chickens, turkeys, geese, ducks, and other pet birds are recognized as direct hosts of Prosthogonimus species; however, P. cuneatus has not been reported to infect peafowl globally. Here, we identify the trematode present in the bursa of the peafowl by both morphological and molecular methods, in addition to the changes in the bursal tissue induced by the parasite, using histopathology. After a necropsy examination, the trematodes were found in the bursa of Fabricius in three peafowl. Morphological and molecular approaches based on taxonomic characteristics and the sequencing of the trematode-specific internal transcribed spacer (ITS) gene, respectively, were employed for trematode identification. The consensus sequences were compared to P. cuneatus reference sequences from GenBank. In order to assess the pathology caused by the parasite, a histological study of the bursa was also performed. Trematodes were confirmed as P. cuneatus based on morphology and DNA sequencing. Further, histopathological evaluation revealed mild lymphoid depletion of the bursal follicles in both the cortex and medulla with associated thinning of the bursal follicular lining epithelium. Indian peafowl can act as a natural host of P. cuneatus. This study provides a detailed pathological and molecular analysis of P. cuneatus affecting Indian peafowl.
期刊介绍:
Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.