犬蔷薇果实水醇提取物对环磷酰胺诱导的小鼠睾丸毒性的保护作用。

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2023-01-01 DOI:10.22038/AJP.2022.20893
Rahmatollah Parandin, Mahnaz Ghowsi, Ahmad Dadbod
{"title":"犬蔷薇果实水醇提取物对环磷酰胺诱导的小鼠睾丸毒性的保护作用。","authors":"Rahmatollah Parandin,&nbsp;Mahnaz Ghowsi,&nbsp;Ahmad Dadbod","doi":"10.22038/AJP.2022.20893","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of <i>Rosa canina</i> L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.</p><p><strong>Materials and methods: </strong>Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29<sup>th</sup> day, sperm parameters, stress oxidative biomarkers, and mRNA expression of <i>nuclear factor erythroid-derived-2 (Nrf2)</i> in testis tissue, as well as blood testosterone were evaluated.</p><p><strong>Results: </strong>The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and <i>Nrf2</i> mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and <i>Nrf2</i> expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and <i>Nrf2</i> expression, and reduced MDA in comparison with the CP group.</p><p><strong>Conclusion: </strong>According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840777/pdf/","citationCount":"1","resultStr":"{\"title\":\"Protective effects of hydroalcoholic extract of <i>Rosa canina</i> L. fruit on cyclophosphamide-induced testicular toxicity in mice.\",\"authors\":\"Rahmatollah Parandin,&nbsp;Mahnaz Ghowsi,&nbsp;Ahmad Dadbod\",\"doi\":\"10.22038/AJP.2022.20893\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of <i>Rosa canina</i> L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.</p><p><strong>Materials and methods: </strong>Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29<sup>th</sup> day, sperm parameters, stress oxidative biomarkers, and mRNA expression of <i>nuclear factor erythroid-derived-2 (Nrf2)</i> in testis tissue, as well as blood testosterone were evaluated.</p><p><strong>Results: </strong>The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and <i>Nrf2</i> mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and <i>Nrf2</i> expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and <i>Nrf2</i> expression, and reduced MDA in comparison with the CP group.</p><p><strong>Conclusion: </strong>According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.</p>\",\"PeriodicalId\":8677,\"journal\":{\"name\":\"Avicenna Journal of Phytomedicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840777/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Avicenna Journal of Phytomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/AJP.2022.20893\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna Journal of Phytomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/AJP.2022.20893","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 1

摘要

目的:报道了环磷酰胺(CP)在受体患者中引起的睾丸毒性。本研究旨在评估犬蔷薇果实水醇提取物(HARF)对CP诱导的BALB/c小鼠睾丸毒性的保护作用。材料和方法:35只小鼠分为五组:第一组(对照组)、第二组(CP,在第1、8、15和22天接受CP 100mg/kg)、第三组(CP+HARF 250mg/kg)、第四组(CP+ARF 500mg/kg)和第五组(CP+HARF 750mg/kg)。在接受CP的第III、IV和V组中,从第1天开始,通过腹膜内注射同时给予HARF连续28天。第29天,评估精子参数、应激氧化生物标志物、睾丸组织中核因子红细胞衍生2(Nrf2)的mRNA表达以及血液睾酮。结果:CP暴露降低了精子参数、超氧化物歧化酶(SOD)活性、睾酮和Nrf2mRNA表达水平,并增加了丙二醛(MDA)。500 mg/kg剂量的HARF改善了精子数量和活力,增加了SOD和过氧化氢酶活性、谷胱甘肽过氧化物酶(GPx)活性、睾酮水平和Nrf2表达,并降低了MDA。此外,与CP组相比,750mg/kg剂量的HARF改善了精子参数,增加了SOD、过氧化氢酶和GPx活性、总睾酮水平和Nrf2表达,并降低了MDA。结论:500和750mg/kg剂量的HARF抑制了CP对小鼠生殖系统的破坏性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Protective effects of hydroalcoholic extract of Rosa canina L. fruit on cyclophosphamide-induced testicular toxicity in mice.

Objective: Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of Rosa canina L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.

Materials and methods: Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29th day, sperm parameters, stress oxidative biomarkers, and mRNA expression of nuclear factor erythroid-derived-2 (Nrf2) in testis tissue, as well as blood testosterone were evaluated.

Results: The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and Nrf2 mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and Nrf2 expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and Nrf2 expression, and reduced MDA in comparison with the CP group.

Conclusion: According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
期刊最新文献
Aloe vera supplementation improves cardiovascular risk factors in hemodialysis patients: A randomized, double-blind, placebo-controlled trial. An in vitro antiviral evaluation of punicalagin toward influenza A virus. Apigenin attenuates serum concentrations of TNF-a, interleukin 1b and interleukin 6 in lipopolysaccharide-stimulated rats. Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy. Effect of pretreatment with Devil's Claw on locomotor activity, infarct volume, and neuronal density in focal cerebral ischemia in rats.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1