秋水仙碱诱导蝴蝶兰小孢子形成过程中的2n花粉。

IF 2 4区 农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2022-09-01 DOI:10.1270/jsbbs.21100
Ting Wu, Xin Zhao, Shuhua Yang, Jiahui Yang, Jun Zhu, Yaping Kou, Xiaonan Yu, Hong Ge, Ruidong Jia
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引用次数: 1

摘要

2n花粉诱导是培育多倍体植物的重要技术。在此,我们观察了6个蝴蝶兰品种的花粉母细胞(PMCs)的减数分裂,并试图诱导2n个花粉。减数分裂阶段与花芽长度有关。在减数分裂过程中,花宽约20 ~ 40 mm和50 ~ 60 mm的蝴蝶兰品种的芽长分别约为3 ~ 8 mm和5 ~ 13 mm。减数分裂持续时间为4.2 ~ 14d,首次对蝴蝶兰PMCs减数分裂进行了研究。2n花粉自然产生频率为0.68% ~ 1.78%。减数分裂阶段和秋水仙碱浓度显著影响2n花粉的诱导。在瘦素-合子素阶段,0.05%秋水仙碱处理3 d,获得2n花粉的频率为10.04%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Induction of 2n pollen with colchicine during microsporogenesis in Phalaenopsis.

The induction of 2n pollen is an important technique for breeding polyploid plants. Here, we observed meiosis in the pollen mother cells (PMCs) of six Phalaenopsis cultivars and attempted to induce 2n pollen. The meiotic stage was related to flower bud length. During meiosis, Phalaenopsis cultivars with flower widths of approximately 20-40 mm and 50-60 mm had bud lengths of approximately 3-8 mm and 5-13 mm, respectively. The duration of meiosis ranged from 4.2 to 14 d. This was the first study to characterize meiosis of the PMCs of Phalaenopsis. The natural generation frequency of 2n pollen varied from 0.68% to 1.78%. Meiotic stage and colchicine concentration significantly affected the induction of 2n pollen. The most effective treatment for obtaining 2n pollen was 0.05% colchicine in the leptotene to zygotene stage for 3 d, which achieved a 2n pollen frequency of 10.04%.

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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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