Fatih Ceran, Ozgur Pilanci, Asuman Ozel, Gul Ilbay, Rukiye Karabacak, Mehmet Kanter, Konuralp Ilbay, Samet Vasfi Kuvat
{"title":"脱细胞真皮基质修复大鼠坐骨神经缺损的实验研究。","authors":"Fatih Ceran, Ozgur Pilanci, Asuman Ozel, Gul Ilbay, Rukiye Karabacak, Mehmet Kanter, Konuralp Ilbay, Samet Vasfi Kuvat","doi":"10.1080/2000656X.2022.2152824","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In patients with nerve tissue defects, the use of autologous nerve grafts is the standard method of treatment. Alternatives to autologous, nerve grafts have attracted the attention of reconstructive surgeons. In this study, the results of nerve repairs using acellular dermal matrix (ADM) in an experimental rat sciatic nerve defect model are presented.</p><p><strong>Methods: </strong>Thirty-six Sprague-Dawley rats were randomized into 5 groups: Group 1: control group, Group 2: negative control group (<i>n</i> = 6), Group 3: autologous nerve graft group (<i>n</i> = 10), Group 4: donor site entubulated with ADM group (<i>n</i> = 10); and Group 5: nerve graft entubulated with ADM group (<i>n</i> = 10). The animals in each group were evaluated for electrophysiologic functions, gastrocnemius muscle weight and histomorphology on the 3rd and 6th month.</p><p><strong>Results: </strong>The compound muscle action potential was observed to be distinctly lower in Groups 3, 4 and 5 in comparison to the control group. In Group 4, the gastrocnemius ratio (GCR) values on the 6th month were statistically significantly lower than the GCR values in Group 3 and Group 5, The histological scores and myelinated axonal counts in Group 5 were statistically significantly higher than the values in Group 3 and Group 4.</p><p><strong>Conclusion: </strong>The results of this study showed that wrapping ADM around nerve grafts resulted in better outcomes with respect to nerve healing.</p>","PeriodicalId":16847,"journal":{"name":"Journal of Plastic Surgery and Hand Surgery","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Use of acellular dermal matrix in peripheral nerve reconstruction: an experimental study on rat sciatic nerve defect.\",\"authors\":\"Fatih Ceran, Ozgur Pilanci, Asuman Ozel, Gul Ilbay, Rukiye Karabacak, Mehmet Kanter, Konuralp Ilbay, Samet Vasfi Kuvat\",\"doi\":\"10.1080/2000656X.2022.2152824\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>In patients with nerve tissue defects, the use of autologous nerve grafts is the standard method of treatment. Alternatives to autologous, nerve grafts have attracted the attention of reconstructive surgeons. In this study, the results of nerve repairs using acellular dermal matrix (ADM) in an experimental rat sciatic nerve defect model are presented.</p><p><strong>Methods: </strong>Thirty-six Sprague-Dawley rats were randomized into 5 groups: Group 1: control group, Group 2: negative control group (<i>n</i> = 6), Group 3: autologous nerve graft group (<i>n</i> = 10), Group 4: donor site entubulated with ADM group (<i>n</i> = 10); and Group 5: nerve graft entubulated with ADM group (<i>n</i> = 10). The animals in each group were evaluated for electrophysiologic functions, gastrocnemius muscle weight and histomorphology on the 3rd and 6th month.</p><p><strong>Results: </strong>The compound muscle action potential was observed to be distinctly lower in Groups 3, 4 and 5 in comparison to the control group. In Group 4, the gastrocnemius ratio (GCR) values on the 6th month were statistically significantly lower than the GCR values in Group 3 and Group 5, The histological scores and myelinated axonal counts in Group 5 were statistically significantly higher than the values in Group 3 and Group 4.</p><p><strong>Conclusion: </strong>The results of this study showed that wrapping ADM around nerve grafts resulted in better outcomes with respect to nerve healing.</p>\",\"PeriodicalId\":16847,\"journal\":{\"name\":\"Journal of Plastic Surgery and Hand Surgery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plastic Surgery and Hand Surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/2000656X.2022.2152824\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ORTHOPEDICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plastic Surgery and Hand Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/2000656X.2022.2152824","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
Use of acellular dermal matrix in peripheral nerve reconstruction: an experimental study on rat sciatic nerve defect.
Background: In patients with nerve tissue defects, the use of autologous nerve grafts is the standard method of treatment. Alternatives to autologous, nerve grafts have attracted the attention of reconstructive surgeons. In this study, the results of nerve repairs using acellular dermal matrix (ADM) in an experimental rat sciatic nerve defect model are presented.
Methods: Thirty-six Sprague-Dawley rats were randomized into 5 groups: Group 1: control group, Group 2: negative control group (n = 6), Group 3: autologous nerve graft group (n = 10), Group 4: donor site entubulated with ADM group (n = 10); and Group 5: nerve graft entubulated with ADM group (n = 10). The animals in each group were evaluated for electrophysiologic functions, gastrocnemius muscle weight and histomorphology on the 3rd and 6th month.
Results: The compound muscle action potential was observed to be distinctly lower in Groups 3, 4 and 5 in comparison to the control group. In Group 4, the gastrocnemius ratio (GCR) values on the 6th month were statistically significantly lower than the GCR values in Group 3 and Group 5, The histological scores and myelinated axonal counts in Group 5 were statistically significantly higher than the values in Group 3 and Group 4.
Conclusion: The results of this study showed that wrapping ADM around nerve grafts resulted in better outcomes with respect to nerve healing.
期刊介绍:
The purpose of the Journal of Plastic Surgery and Hand Surgery is to serve as an international forum for plastic surgery, hand surgery and related research. Interest is focused on original articles on basic research and clinical evaluation.
The scope of the journal comprises:
• Articles concerning operative methods and follow-up studies
• Research articles on subjects related to plastic and hand surgery
• Articles on cranio-maxillofacial surgery, including cleft lip and palate surgery.
Extended issues are published occasionally, dealing with special topics such as microvascular surgery, craniofacial surgery, or burns. Supplements, usually doctoral theses, may also be published.
The journal is published for the Acta Chirurgica Scandinavica society and sponsored by the Key Foundation, Sweden.
The journal was previously published as Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery.