MiRNA-23a-5p是痛风性关节炎的生物标志物，通过诱导TLR2，通过MyD88/NF-κB通路促进痛风性关节炎大鼠的炎症反应。

IF 1.1 4区医学 Q4 Medicine Archives of rheumatology Pub Date : 2022-12-01 DOI:10.46497/ArchRheumatol.2022.9236

Fang Li, Jian-Hua Yao, Li Li, Qian Nie, Jing-Jing Cao, Xiao-Ran Ning

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引用次数: 0

摘要

目的:本研究旨在探讨微核糖核酸(miRNA)-23a-5p在痛风性关节炎中的作用，并探讨其可能的机制。材料与方法:大鼠膝关节腔内注射尿酸钠结晶0.2 mL (20 mg/mL)建立痛风性关节炎模型。采用脂多糖(LPS)诱导THP-1细胞建立体外模型。结果:痛风性关节炎大鼠血清miRNA-23a-5p表达水平升高。然而，miRNA-23a-5p的过表达在体外通过诱导toll样受体-2 (TLR2)促进炎症和诱导髓样差异蛋白-88 (MyD88)/核因子-κB (NF-κB)通路。抑制TLR2可减弱miRNA-23a-5p在痛风性关节炎体外模型炎症中的促炎作用。结论:miRNA-23a-5p是痛风性关节炎的生物标志物，通过MyD88/NF-κB通路靶向TLR2，促进痛风性关节炎大鼠炎症的发生。

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MiRNA-23a-5p is the biomarkers for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by induction TLR2.

Objectives: In this study, we aimed to examine the efficacy of micro ribonucleic acid (miRNA)-23a-5p in gouty arthritis and to investigate its possible mechanism.

Materials and methods: Gouty arthritis in rat was established by intraarticular injection of 0.2 mL monosodium urate crystal (20 mg/mL) inside knee joint cavity. THP-1 cell was induced using lipopolysaccharides (LPS) for in vitro model.

Results: Serum miRNA-23a-5p expression levels were increased in rats of gouty arthritis. However, overexpression of miRNA-23a-5p promoted inflammation and induced myeloid differential protein-88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway by induction toll-like receptor-2 (TLR2) in vitro. The inhibition of TLR2 attenuated the pro-inflammation effects of miRNA-23a-5p in inflammation in in vitro model of gouty arthritis.

Conclusion: Our findings demonstrate that miRNA-23a-5p is a biomarker for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by targeting TLR2.

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来源期刊

Archives of rheumatology Medicine-Rheumatology

CiteScore

2.00

自引率

9.10%

发文量

期刊介绍： The Archives of Rheumatology is an official journal of the Turkish League Against Rheumatism (TLAR) and is published quarterly in March, June, September, and December. It publishes original work on all aspects of rheumatology and disorders of the musculoskeletal system. The priority of the Archives of Rheumatology is to publish high-quality original research articles, especially in inflammatory rheumatic disorders. In addition to research articles, brief reports, reviews, editorials, letters to the editor can also be published. It is an independent peer-reviewed international journal printed in English. Manuscripts are refereed by a "double-blind peer-reviewed" process for both referees and authors. Editorial Board of the Archives of Rheumatology works under the principles of The World Association of Medical Editors (WAME), the International Council of Medical Journal Editors (ICMJE), and Committee on Publication Ethics (COPE).