低密度脂蛋白受体rs5925 (1959C>T)基因多态性在埃及狼疮性肾炎患者血脂异常发病中的作用

IF 1.1 4区医学 Q4 Medicine Archives of rheumatology Pub Date : 2022-12-01 DOI:10.46497/ArchRheumatol.2022.9247

Yumn A Alsabbagh, Saher A Ahmed, Heba E Salama, Mai A Abd-Elmawla, Hala L Elgendy

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引用次数: 1

摘要

目的:研究狼疮性肾炎患者低密度脂蛋白受体(LDL-R) rs5925基因变异的患病率，并探讨其与血脂和肾功能的关系。患者与方法:2020年9月~ 2021年6月，共100例狼疮性肾炎患者(男8例，女92例;平均年龄:31.1±1.1岁;年龄从20岁到67岁不等)，总共100名年龄和性别匹配的健康志愿者(10名男性，90名女性;平均年龄:35.8±2.8岁;范围，21至65岁)。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测rs5925基因多态性。测定血脂和肾功能。结果:狼疮性肾炎患者rs5925 (LDLR)中C等位基因(60%)明显高于对照组(45%)。而T等位基因在狼疮性肾炎患者中明显低于对照组(40%)(p=0.003)。TT和CT基因型狼疮性肾炎患者的血浆总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平明显低于CC基因型狼疮性肾炎患者。此外，TT基因型患者的血浆动脉粥样硬化指数(AIP)和LDL-C/高密度脂蛋白胆固醇(HDL-C)比值明显低于CC基因型患者。肾活检III级、IV级和V级患者与LDLR C等位基因之间存在强烈而明确的相关性(p=0.01, p=0.003, p=0.004)。结论:C等位基因是狼疮性肾炎患者显著流行的LDLR C1959T变异。此外，LDL-R基因变异可能是狼疮性肾炎患者血脂紊乱的非免疫机制之一。严重的血脂异常可能是狼疮性肾炎患者肾功能恶化的部分原因。

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Role of low-density lipoprotein receptor rs5925 (1959C>T) gene polymorphism in pathogenesis of dyslipidemia among Egyptian lupus nephritis patients.

Objectives: This study aims to investigate the prevalence of low-density lipoprotein receptor (LDL-R) rs5925 genetic variants and to evaluate their relationship with plasma lipid and kidney functions in lupus nephritis patients.

Patients and methods: Between September 2020 and June 2021, a total of 100 lupus nephritis patients (8 males, 92 females; mean age: 31.1±1.1 years; range, 20 to 67 years) and a total of 100 age- and sex-matched healthy volunteers (10 males, 90 females; mean age: 35.8±2.8 years; range, 21 to 65 years) were included. The gene polymorphism rs5925 (LDLR) was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Lipid profile and kidney functions were measured.

Results: Regarding rs5925 (LDLR), C allele was significantly higher among lupus nephritis patients (60%) compared to the control group (45%). While T allele was significantly lower in lupus nephritis patients (40%), compared to the control group (p=0.003). The plasma level of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were significantly lower in lupus nephritis patients with TT and CT genotypes, compared to those with CC genotype. Moreover, atherogenic index of plasma (AIP) and LDL-C/high-density lipoprotein cholesterol (HDL-C) ratio were significantly lower in patients with TT genotype, compared to the patients with CC genotype. There was a strong and clear association between patients with renal biopsies grades III & IV & V and LDLR C allele (p=0.01, p=0.003, and p=0.004, respectively).

Conclusion: C allele is the significantly prevailed LDLR C1959T variant among lupus nephritis patients. Moreover, LDL-R genetic variant may be one of the non-immunological mechanisms implicated in the disturbed lipid profile among lupus nephritis patients. Profound dyslipidemia may partly underscore the deterioration of kidney function among lupus nephritis patients.

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来源期刊

Archives of rheumatology Medicine-Rheumatology

CiteScore

2.00

自引率

9.10%

发文量

期刊介绍： The Archives of Rheumatology is an official journal of the Turkish League Against Rheumatism (TLAR) and is published quarterly in March, June, September, and December. It publishes original work on all aspects of rheumatology and disorders of the musculoskeletal system. The priority of the Archives of Rheumatology is to publish high-quality original research articles, especially in inflammatory rheumatic disorders. In addition to research articles, brief reports, reviews, editorials, letters to the editor can also be published. It is an independent peer-reviewed international journal printed in English. Manuscripts are refereed by a "double-blind peer-reviewed" process for both referees and authors. Editorial Board of the Archives of Rheumatology works under the principles of The World Association of Medical Editors (WAME), the International Council of Medical Journal Editors (ICMJE), and Committee on Publication Ethics (COPE).