红树藤茎提取物对病原菌霍乱弧菌的抗氧化和抑菌活性

Angga Crystal Loasana Yami, I. Batubara, K. A. Audah
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引用次数: 2

摘要

背景:一些由自由基和致病菌引起的疾病的治疗通常采用抗氧化剂和抗生素。由于过度使用抗生素和其他环境因素,一些细菌现在对某些抗生素甚至多种抗生素产生耐药性。一些霍乱弧菌菌株对许多抗生素具有多重耐药性。目的:研究木藤茎提取物对霍乱弧菌的抗氧化和抑菌活性。方法:采用梯度浸渍法提取裸子茎。采用DPPH法测定其抗氧化活性,采用圆盘扩散法测定其抗菌活性。采用柱层析法对活性最佳的选择性提取物进行分馏。采用液相色谱-质谱联用法对提取的抗氧化、抗菌活性最佳的化合物进行鉴定。结果:裸子茎乙酸乙酯提取物抑菌活性最佳,MIC和MBC均为62.50 mg/L。乙酸乙酯提取物的抗氧化活性最高,IC50值为255.03 mg/L。馏分试验结果表明,馏分3的抑菌活性和抗氧化活性最好,MIC和MBC值分别为7.90 mg/L和348.91 mg/L。结论:裸子藤茎乙酸乙酯提取物具有良好的抗氧化、抗菌作用。从乙酸乙酯萃取物中提取的抗氧化和抗菌化合物是2-乙基-4-甲基- 1h -咪唑。
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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae
Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.
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