不同营养培养基对芸苔互花菌径向生长的影响Sacc。侵染不同的油菜寄主作物

Vaibhav Singh, R. Khan
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摘要

油菜互花孢菌(Berk.)Sacc。是芸苔科许多芸苔属作物最常见和最具破坏性的病原菌。据世界各大洲的报告,它在作物产品的质量和数量方面造成了相当大的损失。本试验旨在研究马铃薯葡萄糖琼脂(PDA)、V-8果汁琼脂(V8JA)、理查德琼脂(RA)、查佩克Dox琼脂(CDA)和玉米粉琼脂(CMA)等不同营养培养基对芥菜、花椰菜、白菜和萝卜等不同油菜科作物上采集的芸苔菌分离株生长的影响。这些分离株根据其宿主分别被命名为Acae M、Acae Cf、Acae Ca和Acae R。培养7天后观察所有分离株的径向生长。结果表明,不同菌株径向生长差异显著。各菌株在V-8果汁琼脂(V8JA)上生长最快,其次是马铃薯葡萄糖琼脂(PDA),玉米粉琼脂(CMA)上生长最小。
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Effect of different nutrient media on radial growth of Alternaria brassicae (Berk.) Sacc. infecting different Brassica host crops
Alternaria brassicae (Berk.) Sacc. is the most common and destructive pathogen of a number of Brassica crops belong to family Brassicaceae. It has been reported from all the continent of the world and causes considerable losses in terms of quality and quantity of crop produce. The present investigation was undertaken in vitro, to know the effect of different nutrient media viz., Potato Dextrose Agar (PDA), V-8 Juice Agar (V8JA), Richard’s Agar (RA), Czapeck’s Dox Agar (CDA) and Corn Meal Agar (CMA) on the growth of A. brassicae isolates collected from the different Brassica host crops i.e. Mustard, Cauliflower, Cabbage and Radish. These isolates were designated on the basis of their hosts as Acae M, Acae Cf, Acae Ca and Acae R, respectively. Radial growth of all isolates was observed after seven days of incubation. The result showed a marked variation in radial growth of A. brassicae isolates. However, maximum growths of all isolates were recorded in V-8 Juice Agar (V8JA) followed by Potato Dextrose Agar (PDA) while the minimum growth of pathogen was observed in Corn Meal Agar (CMA).
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