{"title":"以酵母菌为参考的气液色谱法定量测定游离氨基酸。","authors":"I Tunblad-Johansson","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.</p>","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 259","pages":"17-24"},"PeriodicalIF":0.0000,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts.\",\"authors\":\"I Tunblad-Johansson\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.</p>\",\"PeriodicalId\":75412,\"journal\":{\"name\":\"Acta pathologica et microbiologica Scandinavica. Supplement\",\"volume\":\" 259\",\"pages\":\"17-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1977-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta pathologica et microbiologica Scandinavica. Supplement\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica et microbiologica Scandinavica. Supplement","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts.
A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.
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