{"title":"离心电场刺激后细胞在微条纹脊上的活性","authors":"S. Hashimoto, Hiroki Yonezawa","doi":"10.1115/imece2021-66412","DOIUrl":null,"url":null,"abstract":"\n Does response of a cell depend on the history of the direction of mechanical stimulation? A mechanical force field generated by centrifugal force is applied to the scaffold plane in the present study. To control the cell direction (0, 45, 90 degrees) with respect to the tangential force, striped micro-ridges in 3 directions were machined on the scaffold surface by photolithography technique. The behavior (movement, and deformation) of each mouse myoblast after cultivation in the tangential force field for 5 hours was tracked for 10 hours by time-lapse images. Experimental results show that the velocity of each cell migration tends to increase regardless of two-dimensional projected area on the scaffold by hysteresis of exposure to tangential force field perpendicular to the longitudinal direction of the cell. The experimental results will lead to the elucidation of the effect of the direction of force stimulation on cells.","PeriodicalId":314012,"journal":{"name":"Volume 5: Biomedical and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Activity of Cell on Micro Stripe Ridges After Force Field Stimulation With Centrifuge\",\"authors\":\"S. Hashimoto, Hiroki Yonezawa\",\"doi\":\"10.1115/imece2021-66412\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Does response of a cell depend on the history of the direction of mechanical stimulation? A mechanical force field generated by centrifugal force is applied to the scaffold plane in the present study. To control the cell direction (0, 45, 90 degrees) with respect to the tangential force, striped micro-ridges in 3 directions were machined on the scaffold surface by photolithography technique. The behavior (movement, and deformation) of each mouse myoblast after cultivation in the tangential force field for 5 hours was tracked for 10 hours by time-lapse images. Experimental results show that the velocity of each cell migration tends to increase regardless of two-dimensional projected area on the scaffold by hysteresis of exposure to tangential force field perpendicular to the longitudinal direction of the cell. The experimental results will lead to the elucidation of the effect of the direction of force stimulation on cells.\",\"PeriodicalId\":314012,\"journal\":{\"name\":\"Volume 5: Biomedical and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Volume 5: Biomedical and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1115/imece2021-66412\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Volume 5: Biomedical and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1115/imece2021-66412","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Activity of Cell on Micro Stripe Ridges After Force Field Stimulation With Centrifuge
Does response of a cell depend on the history of the direction of mechanical stimulation? A mechanical force field generated by centrifugal force is applied to the scaffold plane in the present study. To control the cell direction (0, 45, 90 degrees) with respect to the tangential force, striped micro-ridges in 3 directions were machined on the scaffold surface by photolithography technique. The behavior (movement, and deformation) of each mouse myoblast after cultivation in the tangential force field for 5 hours was tracked for 10 hours by time-lapse images. Experimental results show that the velocity of each cell migration tends to increase regardless of two-dimensional projected area on the scaffold by hysteresis of exposure to tangential force field perpendicular to the longitudinal direction of the cell. The experimental results will lead to the elucidation of the effect of the direction of force stimulation on cells.