转移性乳腺癌抽吸物中GATA3、GCDFP15、Mammaglobin和SOX10免疫细胞化学的比较

J. Li, J. Ng, C. Lee, Cheuk-Yin Tang, J. Tsang, G. Tse
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引用次数: 2

摘要

转移性癌症经常通过细针穿刺(FNA)细胞学检测到,而转移性乳腺癌的确认通常需要免疫细胞化学。为FNA标本提供组织是很重要的。在本研究中,对组织学证实的转移性乳腺癌的抽吸液细胞阻断制剂进行GATA3、总囊性疾病液蛋白-15 (GCDFP15)、mammaglobin (MMG)和SOX10检测。研究了单个标记物和这些标记物的组合的诊断性能,目的是构建一个组织高效的免疫板。方法:收集具有相应组织学和生物标志物(雌激素受体(ER)、孕激素受体(PR)、HER2和ki67)谱的转移性乳腺癌抽吸标本。在细胞块切片上进行ER、GATA3、GCDFP15、MMG和SOX10免疫染色,并对其表达进行评估和比较。结果:共对115例抽吸物进行免疫染色。表达量最高的是GATA3,其次是MMG、GCDFP15和SOX10。仅表达GATA3、MMG和SOX10的分别为23例、5例和5例。5例仅表达SOX10的患者均为ER阴性,SOX10表达与ER (p = 0.001)、MMG (p = 0.001)、GCDFP15 (p = 0.010)、GATA3 (p = 0.002)呈负相关,而GATA3表达与ER阳性呈正相关(p < 0.001)。MMG和GCDFP15与Ki67高表达相关(p < 0.05),与HER2表达无相关性。结论:在该队列中,GATA3是最敏感的单一标志物。MMG和SOX10的加入分别提高了ER阳性和ER阴性乳腺癌的检测灵敏度。这些发现支持使用GATA3/MMG/SOX10联合检测来确诊转移性乳腺癌。
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Comparison of GATA3, GCDFP15, Mammaglobin and SOX10 Immunocytochemistry in Aspirates of Metastatic Breast Cancer
Introduction: Metastatic cancers are frequently detected on fine-needle aspiration (FNA) cytology, and confirmation of metastatic breast cancer often requires immunocytochemistry. Tissue provisioning for FNA specimens is important. In this study, GATA3, gross cystic disease fluid protein-15 (GCDFP15), mammaglobin (MMG), and SOX10 were performed on cell block preparations from aspirates of histologically confirmed metastatic breast cancers. The diagnostic performance of single markers and combinations of these markers were investigated with the aim to construct a tissue-efficient immunopanel. Methodology: Aspirates of metastatic breast cancer with corresponding histology and biomarker (estrogen receptor (ER), progesterone receptor (PR), HER2 and ki67) profile were retrieved. ER, GATA3, GCDFP15, MMG and SOX10 immunostains were performed on cell block sections and their expressions were assessed and compared. Results: Immunostaining was performed on a total of 115 aspirates. GATA3 showed the highest expression, followed by MMG, GCDFP15 and SOX10. Twenty-three, five and five cases expressed GATA3, MMG and SOX10 only. The five cases expressing SOX10 only were ER negative, and SOX10 expression was negatively associated with ER (p = 0.001), MMG (p = 0.001), GCDFP15 (p = 0.010) and GATA3 (p = 0.002), whereas GATA3 expression showed positive correlation with ER positivity (p < 0.001). MMG and GCDFP15 showed association with high Ki67 (p < 0.05), and no correlations were found with HER2 expression. Conclusion: In this cohort, GATA3 was the most sensitive single marker. The addition of MMG and SOX10 increases the sensitivity for detection of ER positive and ER negative breast cancers, respectively. These findings support the use of a combination of GATA3/MMG/SOX10 for confirmation of metastatic breast cancer.
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