Biological Activity of Natural Formulation with Propolis, Lavender and Thyme Oil on Candida Species

Objective: Candida strains have been frequently associated with nosocomial infections in recent years. On the other hand, public prefer natural resources rather than synthetic materials. Therefore, it is important to research combine effects of natural products. This study aimed to investigate the antifungal and antibiofilm effectiveness of essential oils and propolis containing samples on Candida species and the toxicity of samples. Material-Method: Three different samples were prepared with propolis, lavender oil and thyme oil. Gas Chromatography-mass Spectrometry (GC/MS) GC/MS was used for the determination of organic compounds in samples. The antifungal effects of a natural product mix were tested against C. albicans, C. krusei and C. parapsilosis by disk diffusion method. MIC and MFC tests of the mixture were performed against a standard such as Nystatin. Antibiofilm tests were performed with the microplate system, taking into account the detected concentrations. Finally, in the cytotoxicity test investigated in the L929 cell line with the WST-1 kit. Results: C. albicans was determined as the most sensitive species (MIC50: 90 ppm, MIC90: 750 ppm MFC: 3120 ppm). In the antibiofilm tests, it was determined that the sample was more effective on C. albicans and it inhibited biofilm formation. Also it was determined the IC50 value was 5052 ppm. Conclusion: The natural product mixture, which has scientifically proven antifungal effect, has a feature that has high added value and contributes to the economy. But of course, it has to investigate by further studies. The findings of this study may lead to new therapeutic approaches with standardized natural products in the future.