不同保存液和底物对肾小管代谢活力的影响。

W Fischer, F Manz, K Schärer
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引用次数: 0

摘要

葡萄糖生成、丙酮酸摄取和乳酸生成作为低温保存的离体大鼠肾小管的代谢活力测试。结果取决于所使用的保存溶液的类型。物种特异性血清是维持细胞正常代谢水平的唯一解决方案。使用Collins溶液,所有的活力参数都显示最低的结果。在Krebs-Henseledt溶液中添加某些底物可提高代谢活力。
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Effect of different preservation solutions and substrates on metabolic viability of kidney tubules.

Glucose production, pyruvate uptake and lactate production were taken as metabolic viability tests for isolated rat kidney tubules preserved in hypothermia. The results depend on the type of preservation solution used. Species specific serum is the only solution sustaining cellular metabolism at a normal level. Using Collins solution all viability parameters showed the lowest results. Addition of certain substrates to the Krebs-Henseledt solution improves metabolic viability.

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